A self-assembled multi-enzyme nanomachine and whole-cell biocatalyst for the sustainable valorization of marine biomass into fermentable sugars and consolidated bioprocessing

Marine biomass is a potential resource that contains functional carbohydrates, such as agar, carrageenan, and cellulose, and can be modified for various applications. A novel self-assembled multi-enzyme nanomachine (SAMN) and its whole-cell biocatalyst were developed to target the degradation of mar...

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Veröffentlicht in:International journal of biological macromolecules Jg. 322; H. Pt 3; S. 146853
Hauptverfasser: Lee, Myeong-Eun, Bhardwaj, Nisha, Cho, Byeong-Hyeon, Hyeon, Jeong Eun, Jeong, Wu-Young, Han, Sung Ok
Format: Journal Article
Sprache:Englisch
Veröffentlicht: Netherlands Elsevier B.V 01.09.2025
Schlagworte:
Agar - chemistry
Aquatic Organisms
Biocatalysis
Biomass
Biomass-to-biofuel conversion
Carbohydrate Metabolism
Carrageenan - chemistry
Cellulose - chemistry
Fermentation
Hydrolysis
Marine carbohydrate-active enzymes
Marine polysaccharide
Modular enzyme assembly
Rhodophyta - chemistry
Saccharomyces cerevisiae - metabolism
Sugars - metabolism
Surface-engineered microbial catalyst
Marine polysaccharide
Modular enzyme assembly
Biomass-to-biofuel conversion
Marine carbohydrate-active enzymes
Surface-engineered microbial catalyst
ISSN:0141-8130, 1879-0003, 1879-0003
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Zusammenfassung:Marine biomass is a potential resource that contains functional carbohydrates, such as agar, carrageenan, and cellulose, and can be modified for various applications. A novel self-assembled multi-enzyme nanomachine (SAMN) and its whole-cell biocatalyst were developed to target the degradation of marine biomass components and enable consolidated bioprocessing. Compared to each representative single enzyme, the marine biomass-degrading SAMN exhibited an average 1.45-fold higher hydrolytic activity for six of the seven substrates. The modification of SAMN with MglB further enhanced the hydrolytic activities toward agar, agarose, lambda-carrageenan, and agar-carrageenans-cellulose mixed substrate. Moreover, SAMN displayed a maximum thermostability increase of 14.10%. The SAMN and the SAMN with MglB produced a maximum of 2.25 ± 0.04 mM and 2.33 ± 0.03 mM galactose, a functional sugar from real red algae, respectively. The Saccharomyces cerevisiae-based whole-cell biocatalyst displaying SAMN with MglB produced 2.00 ± 0.14 g/L ethanol from real red algae as the sole carbon source, achieving the highest production among all samples. This marine biomass-degrading SAMN and its whole-cell biocatalyst have potential applications in the saccharification and consolidated bioprocessing of marine biomass, leading to the production of functional sugars and value-added bio-based products in industrial settings. [Display omitted] •Self-assembled multi-enzyme nanomachine (SAMN) was newly developed.•SAMN showed enhanced hydrolysis activity toward marine biomass-related substrates.•SAMN was thermostable in the condition of high temperature.•The modified SAMN produced maximum 2.33 ± 0.03 mM galactose from real red algae.•The SAMN-displaying whole-cell biocatalyst produced 2.00 ± 0.14 g/L ethanol from real red algae.
Bibliographie:ObjectType-Article-1
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content type line 23
ISSN:0141-8130
1879-0003
1879-0003
DOI:10.1016/j.ijbiomac.2025.146853