The relationship between matrix metalloproteinases (MMP-3, -8, -9) in serum and peripheral lymphocytes (CD8+, CD56+) in Down syndrome children with gingivitis

Background and Objective Altered immune response may be a major contributor to periodontal disease in Down syndrome. This study investigated the relationship between peripheral lymphocytes and matrix metalloproteinases (MMPs) in serum in Down syndrome children with gingivitis. Material and Methods C...

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Vydáno v:Journal of periodontal research Ročník 49; číslo 6; s. 742 - 750
Hlavní autoři: Tsilingaridis, G., Yucel-Lindberg, T., Concha Quezada, H., Modéer, T.
Médium: Journal Article
Jazyk:angličtina
Vydáno: United States Blackwell Publishing Ltd 01.12.2014
Témata:
Adolescent
CD56 Antigen - analysis
CD8 Antigens - analysis
CD8-Positive T-Lymphocytes - immunology
Child
crevicular fluid
Cross-Sectional Studies
Down syndrome
Down Syndrome - blood
Down Syndrome - enzymology
Down Syndrome - immunology
Female
Gingival Crevicular Fluid - enzymology
Gingival Crevicular Fluid - immunology
Gingivitis - blood
Gingivitis - enzymology
Gingivitis - immunology
Humans
Killer Cells, Natural - immunology
Lipopolysaccharides - pharmacology
Male
Matrix Metalloproteinase 3 - blood
Matrix Metalloproteinase 8 - blood
Matrix Metalloproteinase 9 - blood
matrix metalloproteinases
Pilot Projects
Porphyromonas gingivalis
serum
T cells
Tissue Inhibitor of Metalloproteinase-1 - blood
Tissue Inhibitor of Metalloproteinase-2 - blood
Tissue Inhibitor of Metalloproteinase-3 - blood
Young Adult
crevicular fluid
Down syndrome
T cells
serum
matrix metalloproteinases
ISSN:0022-3484, 1600-0765, 1600-0765
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Shrnutí:Background and Objective Altered immune response may be a major contributor to periodontal disease in Down syndrome. This study investigated the relationship between peripheral lymphocytes and matrix metalloproteinases (MMPs) in serum in Down syndrome children with gingivitis. Material and Methods Children with Down syndrome (n = 10) and healthy controls (n = 10) were clinically and radiographically examined during dental treatment under general anaesthesia. Peripheral blood and gingival crevicular fluid were collected from each subject and concentrations were determined: serum MMP‐2, ‐3, ‐8 and ‐9; serum tissue inhibitors of metalloproteinases (TIMP) ‐1, ‐2 and ‐3; and gingival crevicular fluid. Leukocytes were isolated from peripheral blood and the relative amounts (%) of the various cell phenotypes were analysed using flow cytometry. In addition, peripheral blood cells were treated with Porphyromonas gingivalis lipopolysaccharide and levels of MMPs and TIMPs measured. Results Concentrations of MMP‐3, MMP‐8 and TIMP‐1 in serum were significantly higher (p < 0.05) in the Down syndrome group compared to the controls. When peripheral blood leukocytes were cultured in the presence or absence of P. gingivalis lipopolysaccharide, MMP‐8 levels were significantly (p < 0.05) higher in the Down syndrome group compared to controls. Children with Down syndrome exhibited significant positive correlations between CD8+ T cells and MMP‐8 (r = 0.630; p = 0.050), between CD8+ T cells and MMP‐9 (r = 0.648; p = 0.043), and between CD56+ NK cells and MMP‐3 (r = 0.828; p = 0.003) compared to controls. Conclusions The positive relationship of serum MMP‐3, ‐8 and ‐9 with immune cells in children with Down syndrome may facilitate migration of CD8+ T cells and CD56+ NK cells into the periodontal tissue, which may contribute to the increased degradation of periodontal tissue in individuals with Down syndrome.
Bibliografie:istex:6C29E950428EE166A1E6D119ED453DA80EB7A611
ArticleID:JRE12157
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ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ObjectType-Article-1
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ISSN:0022-3484
1600-0765
1600-0765
DOI:10.1111/jre.12157