Toxicological evaluation of protein powder derived from Cupriavidus necator

Microorganisms have the potential to produce nutrient‐rich products that can be consumed as food or feed. The protein‐rich powder derived from heat treatment of the whole‐cell biomass of polyhydroxybutyrate‐deficient Cupriavidus necator, a metabolically versatile organism that uses elements found in...

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Vydáno v:Journal of applied toxicology Ročník 43; číslo 6; s. 887 - 912
Hlavní autoři: Modica, Vickie, Glávits, Róbert, Murbach, Timothy S., Endres, John R., Hirka, Gábor, Vértesi, Adél, Béres, Erzsébet, Pasics Szakonyiné, Ilona
Médium: Journal Article
Jazyk:angličtina
Vydáno: England Wiley Subscription Services, Inc 01.06.2023
Témata:
Animals
Biocompatibility
Chromosome aberrations
Cupriavidus necator
Female
Food
Genotoxicity
Heat treatment
Heat treatments
Humans
In vitro methods and tests
In vivo methods and tests
Male
Mammals
Microorganisms
Mutagenicity
Mutagenicity Tests
No-Observed-Adverse-Effect Level
NOAEL
Polyhydroxybutyrate
Polyhydroxybutyric acid
Powder
Powders - toxicity
protein concentrate
Proteins
Rats
Rats, Wistar
Side effects
single‐cell protein
Toxicity
toxicity, genotoxicity, safety
Cupriavidus necator
single-cell protein
toxicity, genotoxicity, safety
NOAEL
protein concentrate
ISSN:0260-437X, 1099-1263, 1099-1263
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Shrnutí:Microorganisms have the potential to produce nutrient‐rich products that can be consumed as food or feed. The protein‐rich powder derived from heat treatment of the whole‐cell biomass of polyhydroxybutyrate‐deficient Cupriavidus necator, a metabolically versatile organism that uses elements found in the air, is an example of such a product. To assess the safety of the protein powder for use as a nutritional ingredient in human food, in accordance with internationally accepted standards, its genotoxic potential and repeated‐dose oral toxicity were investigated. A bacterial reverse mutation test, an in vitro mammalian chromosomal aberration test, and an in vivo mammalian micronucleus test were performed. No evidence of mutagenicity or genotoxicity was found. Additionally, a 90‐day repeated‐dose oral toxicity study in rats was completed, in which a total of 100 male and female Wistar rats were exposed by gavage to daily doses of 1000, 2000, or 3000 mg/kg bw/day of the test material. Following 90 days of continuous exposure, no mortality or treatment‐related adverse effects were observed and no target organs were identified. Therefore, a no observed adverse effect level was determined at 3000 mg/kg bw/day, the highest dose tested. The whole‐cell biomass of polyhydroxybutyrate‐deficient Cupriavidus necator is protein rich. The derived protein concentrate has the potential for use as an environmentally sustainable nutrient‐dense food ingredient. Therefore, we assessed the genotoxic potential and oral toxicity of the heat‐treated and powdered product, in preclinical safety studies. No evidence of genotoxicity or mutagenicity was found. In a 90‐day repeated‐dose oral toxicity study, a no observed adverse effect level in rats of 3000 mg/kg bw/day, the highest feasible dose, was determined.
Bibliografie:Funding information
Adél Vértesi, Erzsébet Béres, and Ilona Pasics Szakonyiné are co‐senior authors.
The authors disclose that financial support for the research described herein was provided by Kiverdi, Inc., Pleasanton, CA.
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ISSN:0260-437X
1099-1263
1099-1263
DOI:10.1002/jat.4432