Syrian hamster embryo (SHE) assay (pH 6.7) coupled with infrared spectroscopy and chemometrics towards toxicological assessment
The Syrian hamster embryo (SHE) assay (pH 6.7) is an in vitro candidate to replace in vivo carcinogenicity tests. However, the conventional method of visual scoring of foci (non-transformed vs. transformed colonies) can be time-consuming and is open to subjectivity. Infrared (IR) spectroscopy has th...
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| Published in: | Analyst (London) Vol. 135; no. 12; p. 3266 |
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| Main Authors: | , , , , , , , , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
England
01.12.2010
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| ISSN: | 1364-5528, 1364-5528 |
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| Abstract | The Syrian hamster embryo (SHE) assay (pH 6.7) is an in vitro candidate to replace in vivo carcinogenicity tests. However, the conventional method of visual scoring of foci (non-transformed vs. transformed colonies) can be time-consuming and is open to subjectivity. Infrared (IR) spectroscopy has the potential to provide objective assessment of such SHE colonies with the added advantage of potentially providing mechanistic information. In this study, SHE cells were treated with one of eight different chemical regimens, allowed in culture to attach and form foci on IR-reflective glass slides; these were subsequently interrogated by attenuated total reflection (ATR) Fourier-transform IR (FTIR) spectroscopy. Derived mid-IR spectra (n = 13,406) were subjected to chemometric analysis focusing primarily on the extraction of biochemical information related to test agent treatment and/or morphological transformation. The use of ATR-FTIR spectroscopy with chemometrics to analyze the SHE assay is a novel approach to toxicological assessment. |
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| AbstractList | The Syrian hamster embryo (SHE) assay (pH 6.7) is an in vitro candidate to replace in vivo carcinogenicity tests. However, the conventional method of visual scoring of foci (non-transformed vs. transformed colonies) can be time-consuming and is open to subjectivity. Infrared (IR) spectroscopy has the potential to provide objective assessment of such SHE colonies with the added advantage of potentially providing mechanistic information. In this study, SHE cells were treated with one of eight different chemical regimens, allowed in culture to attach and form foci on IR-reflective glass slides; these were subsequently interrogated by attenuated total reflection (ATR) Fourier-transform IR (FTIR) spectroscopy. Derived mid-IR spectra (n = 13,406) were subjected to chemometric analysis focusing primarily on the extraction of biochemical information related to test agent treatment and/or morphological transformation. The use of ATR-FTIR spectroscopy with chemometrics to analyze the SHE assay is a novel approach to toxicological assessment. The Syrian hamster embryo (SHE) assay (pH 6.7) is an in vitro candidate to replace in vivo carcinogenicity tests. However, the conventional method of visual scoring of foci (non-transformed vs. transformed colonies) can be time-consuming and is open to subjectivity. Infrared (IR) spectroscopy has the potential to provide objective assessment of such SHE colonies with the added advantage of potentially providing mechanistic information. In this study, SHE cells were treated with one of eight different chemical regimens, allowed in culture to attach and form foci on IR-reflective glass slides; these were subsequently interrogated by attenuated total reflection (ATR) Fourier-transform IR (FTIR) spectroscopy. Derived mid-IR spectra (n = 13,406) were subjected to chemometric analysis focusing primarily on the extraction of biochemical information related to test agent treatment and/or morphological transformation. The use of ATR-FTIR spectroscopy with chemometrics to analyze the SHE assay is a novel approach to toxicological assessment.The Syrian hamster embryo (SHE) assay (pH 6.7) is an in vitro candidate to replace in vivo carcinogenicity tests. However, the conventional method of visual scoring of foci (non-transformed vs. transformed colonies) can be time-consuming and is open to subjectivity. Infrared (IR) spectroscopy has the potential to provide objective assessment of such SHE colonies with the added advantage of potentially providing mechanistic information. In this study, SHE cells were treated with one of eight different chemical regimens, allowed in culture to attach and form foci on IR-reflective glass slides; these were subsequently interrogated by attenuated total reflection (ATR) Fourier-transform IR (FTIR) spectroscopy. Derived mid-IR spectra (n = 13,406) were subjected to chemometric analysis focusing primarily on the extraction of biochemical information related to test agent treatment and/or morphological transformation. The use of ATR-FTIR spectroscopy with chemometrics to analyze the SHE assay is a novel approach to toxicological assessment. |
| Author | Pollock, Hubert M Llabjani, Valon Martin, Francis L Cheung, Karen T Pant, Kamala Najand, Ghazal M Patel, Imran I Trevisan, Júlio Carmichael, Paul L Angelov, Plamen P Bruce, Shannon W Scott, Andrew D |
| Author_xml | – sequence: 1 givenname: Júlio surname: Trevisan fullname: Trevisan, Júlio organization: School of Computing and Communication Systems, Lancaster University, Lancaster, LA1 4WA, UK – sequence: 2 givenname: Plamen P surname: Angelov fullname: Angelov, Plamen P – sequence: 3 givenname: Imran I surname: Patel fullname: Patel, Imran I – sequence: 4 givenname: Ghazal M surname: Najand fullname: Najand, Ghazal M – sequence: 5 givenname: Karen T surname: Cheung fullname: Cheung, Karen T – sequence: 6 givenname: Valon surname: Llabjani fullname: Llabjani, Valon – sequence: 7 givenname: Hubert M surname: Pollock fullname: Pollock, Hubert M – sequence: 8 givenname: Shannon W surname: Bruce fullname: Bruce, Shannon W – sequence: 9 givenname: Kamala surname: Pant fullname: Pant, Kamala – sequence: 10 givenname: Paul L surname: Carmichael fullname: Carmichael, Paul L – sequence: 11 givenname: Andrew D surname: Scott fullname: Scott, Andrew D – sequence: 12 givenname: Francis L surname: Martin fullname: Martin, Francis L |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/20938551$$D View this record in MEDLINE/PubMed |
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| Snippet | The Syrian hamster embryo (SHE) assay (pH 6.7) is an in vitro candidate to replace in vivo carcinogenicity tests. However, the conventional method of visual... |
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| SubjectTerms | Animals Biological Assay - instrumentation Biological Assay - methods Cell Transformation, Neoplastic - drug effects Cricetinae Discriminant Analysis Embryo, Mammalian - cytology Embryo, Mammalian - drug effects Mesocricetus - embryology Organic Chemicals - pharmacology Principal Component Analysis Spectroscopy, Fourier Transform Infrared - instrumentation Spectroscopy, Fourier Transform Infrared - methods |
| Title | Syrian hamster embryo (SHE) assay (pH 6.7) coupled with infrared spectroscopy and chemometrics towards toxicological assessment |
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