Isolation and characterization of a novel lectin with antifungal and antiproliferative activities from Sophora alopecuroides seeds

Sophora alopecuroides lectin (SAL), a novel lectin from the seeds of Sophora alopecuroides, was purified by ion-exchange chromatography on diethylaminoethyl (DEAE)- and carboxymethyl (CM)-Sepharose columns, followed by gel filtration on a Sephadex 75 10/300 GL column. SAL was found to be a monomer o...

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Published in:Acta biochimica et biophysica Sinica Vol. 44; no. 7; p. 606
Main Authors: Li, Tingting, Yin, Xiaoli, Liu, Dongliang, Ma, Xiaojin, Lv, Hui, Sun, Surong
Format: Journal Article
Language:English
Published: China 01.07.2012
Subjects:
Alternaria - drug effects
Alternaria - growth & development
Amino Acid Sequence
Animals
Cell Line, Tumor
Cell Proliferation - drug effects
Chromatography, High Pressure Liquid
Dose-Response Relationship, Drug
Electrophoresis, Polyacrylamide Gel
Fungi - drug effects
Fungi - growth & development
Glycine - analogs & derivatives
Glycine - chemistry
HeLa Cells
Hemagglutination Tests
Humans
Inhibitory Concentration 50
Mycelium - drug effects
Mycelium - growth & development
Penicillium - drug effects
Penicillium - growth & development
Plant Lectins - genetics
Plant Lectins - isolation & purification
Plant Lectins - pharmacology
Rabbits
Seeds - chemistry
Sophora - chemistry
ISSN:1745-7270, 1745-7270
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Summary:Sophora alopecuroides lectin (SAL), a novel lectin from the seeds of Sophora alopecuroides, was purified by ion-exchange chromatography on diethylaminoethyl (DEAE)- and carboxymethyl (CM)-Sepharose columns, followed by gel filtration on a Sephadex 75 10/300 GL column. SAL was found to be a monomer of 39916.3 Da, as determined by tricine-sodium dodecyl sulphate-polyacrylamide gel electrophoresis and high-performance liquid chromatography (HPLC). The N-terminal 10-amino acid sequence of SAL, KPWALSFSFG, resembles those of other legume lectins. SAL exhibits hemagglutinating activity against rabbit erythrocytes at 11.9 μg/ml. Its hemagglutinating activity is stable in the pH range 7-11 and in the temperature range 30-90°C, and is stimulated by Mn(2+). The hemagglutinating activity of SAL is most potently inhibited by 50-mM d-galactose. SAL suppresses mycelial growth in Penicillium digitatum and Alternaria alternata; the IC(50) of the antifungal activity toward P. digitatum and A. alternata were found to be 3.125 and 3.338 μM, respectively. SAL suppresses the proliferation of human cervical cancer cells (HeLa) at an IC(50) of 6.25 μM (P< 0.05). But it has no inhibiting effect on bacteria. This is the first report of a lectin from seeds of S. alopecuroides.
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ISSN:1745-7270
1745-7270
DOI:10.1093/abbs/gms037