Morphological characterization of the levator veli palatini muscle in children born with cleft palates

The aim of this study was to analyze, morphologically and biochemically, one of the soft palate muscles, the levator veli palatini (LVP), in children born with cleft palate. Biopsies were obtained from nine male and three female infants in connection with the early surgical repair of the hard and so...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:The Cleft palate-craniofacial journal Jg. 38; H. 5; S. 438
Hauptverfasser: Lindman, R, Paulin, G, Stål, P S
Format: Journal Article
Sprache:Englisch
Veröffentlicht: United States 01.09.2001
Schlagworte:
Adenosine Triphosphatases - analysis
Adult
Antibodies, Monoclonal
Biopsy
Capillaries - pathology
Cleft Palate - metabolism
Cleft Palate - pathology
Connective Tissue - pathology
Female
Histocytochemistry
Humans
Immunoblotting
Immunohistochemistry
Infant
Laminin - analysis
Male
Mitochondria, Muscle - enzymology
Muscle Fibers, Fast-Twitch - ultrastructure
Muscle Fibers, Slow-Twitch - ultrastructure
Muscle Spindles - ultrastructure
Myosin Heavy Chains - analysis
NAD - analysis
Palatal Muscles - chemistry
Palatal Muscles - pathology
Palate, Soft - chemistry
Palate, Soft - pathology
Statistics as Topic
Velopharyngeal Insufficiency - pathology
ISSN:1055-6656
Online-Zugang:Weitere Angaben
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:The aim of this study was to analyze, morphologically and biochemically, one of the soft palate muscles, the levator veli palatini (LVP), in children born with cleft palate. Biopsies were obtained from nine male and three female infants in connection with the early surgical repair of the hard and soft palate. Samples from five adult normal LVP muscles were used for comparison. The muscle morphology, fiber type and myosin heavy chain (MyHC) compositions, capillary supply, and content of muscle spindles were analyzed with different enzyme-histochemical, immunohistochemical, and biochemical techniques. Compared with the normal adult subjects, the LVP muscle from the infantile subjects with cleft had a smaller mean fiber diameter, a larger variability in fiber size and form, a higher proportion of type II fibers, a higher amount of fast MyHCs, and a lower density of capillaries. No muscle spindles were observed. Moreover, one-third of the biopsies from the infantile subjects with cleft LVP either lacked muscle tissue or contained only a small amount. The LVP muscle from children with cleft palate has a different morphology, compared with the normal adult muscle. The differences might be related to different stages in maturation of the muscles, changes in functional demands with growth and age, or a consequence of the cleft. The lack of contractile tissue in some of the cleft biopsies offers one possible explanation to a persistent postsurgical velopharyngeal insufficiency in some patients, despite a successful surgical repair.
ISSN:1055-6656
DOI:10.1597/1545-1569(2001)038<0438:MCOTLV>2.0.CO;2