Diazotroph Community Characterization via a High-Throughput nifH Amplicon Sequencing and Analysis Pipeline

The dinitrogenase reductase gene ( ) is the most widely established molecular marker for the study of nitrogen-fixing prokaryotes in nature. A large number of PCR primer sets have been developed for amplification, and the effective deployment of these approaches should be guided by a rapid, easy-to-...

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Bibliographic Details
Published in:	Applied and environmental microbiology Vol. 84; no. 4
Main Authors:	Gaby, John Christian, Rishishwar, Lavanya, Valderrama-Aguirre, Lina C, Green, Stefan J, Valderrama-Aguirre, Augusto, Jordan, I King, Kostka, Joel E
Format:	Journal Article
Language:	English
Published:	United States 15.02.2018
Subjects:	Bacteria - classification Bacteria - genetics Bacteria - metabolism Bacterial Physiological Phenomena Computational Biology DNA, Bacterial - genetics Ecosystem High-Throughput Nucleotide Sequencing - methods Metagenomics Microbiota - genetics Microbiota - physiology Nitrogen - metabolism Nitrogen Fixation Oxidoreductases - genetics Phylogeny Polymerase Chain Reaction Rhizosphere Sequence Analysis, DNA Soil Microbiology sequence analysis taxonomy nitrogen fixation next-generation sequencing metagenomics bioinformatics
ISSN:	1098-5336, 1098-5336
Online Access:	Get more information
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