Separation of Mupirocin by Normal Phase Liquid Chromatography (NPLC)

Mupirocin is an antibiotic from Pseudomonas fluorescens used topically against Staphylococci infections. There are many patented strategies which are proposed for separation of the antibiotic from the fermentation broth comprising of liquid-liquid extraction and hydrophobic chromatography. The paper...

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Veröffentlicht in:Separation science and technology Jg. 49; H. 18; S. 2907 - 2912
Hauptverfasser: Patel, Jalpa, Desai, Gaurav, Upadhya, Archana
Format: Journal Article
Sprache:Englisch
Veröffentlicht: Taylor & Francis 12.12.2014
Schlagworte:
Adsorbents
antibiotic
Antibiotics
Chromatography
Elution
High performance liquid chromatography
Liquid chromatography
mupirocin
Pseudomonas fluorescens
Separation
ISSN:0149-6395, 1520-5754
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Abstract Mupirocin is an antibiotic from Pseudomonas fluorescens used topically against Staphylococci infections. There are many patented strategies which are proposed for separation of the antibiotic from the fermentation broth comprising of liquid-liquid extraction and hydrophobic chromatography. The paper demonstrates the use of native silica, as used in flash chromatography, as adsorbent for normal phase liquid chromatography of mupirocin. Mobile phase for elution was acidified n-hexane: ethyl acetate (10/90 v/v). The method achieved a yield of 66% relative to the mupirocin source and a concentration factor of 13. Anti-microbial assays, high performance liquid chromatography and Fourier transform infrared spectroscopy has been used to confirm the identity of the antibiotic after chromatographic separation. An alternative route to isolation of mupirocin using the polar sorbent has been suggested.
AbstractList Mupirocin is an antibiotic from Pseudomonas fluorescens used topically against Staphylococci infections. There are many patented strategies which are proposed for separation of the antibiotic from the fermentation broth comprising of liquid-liquid extraction and hydrophobic chromatography. The paper demonstrates the use of native silica, as used in flash chromatography, as adsorbent for normal phase liquid chromatography of mupirocin. Mobile phase for elution was acidified n-hexane: ethyl acetate (10/90 v/v). The method achieved a yield of 66% relative to the mupirocin source and a concentration factor of 13. Anti-microbial assays, high performance liquid chromatography and Fourier transform infrared spectroscopy has been used to confirm the identity of the antibiotic after chromatographic separation. An alternative route to isolation of mupirocin using the polar sorbent has been suggested.
Author Desai, Gaurav
Upadhya, Archana
Patel, Jalpa
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Cites_doi 10.1038/nrmicro2278
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10.1074/jbc.273.48.31691
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Snippet Mupirocin is an antibiotic from Pseudomonas fluorescens used topically against Staphylococci infections. There are many patented strategies which are proposed...
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SubjectTerms Adsorbents
antibiotic
Antibiotics
Chromatography
Elution
High performance liquid chromatography
Liquid chromatography
mupirocin
Pseudomonas fluorescens
Separation
Title Separation of Mupirocin by Normal Phase Liquid Chromatography (NPLC)
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