Separation of Mupirocin by Normal Phase Liquid Chromatography (NPLC)

Mupirocin is an antibiotic from Pseudomonas fluorescens used topically against Staphylococci infections. There are many patented strategies which are proposed for separation of the antibiotic from the fermentation broth comprising of liquid-liquid extraction and hydrophobic chromatography. The paper...

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Vydáno v:Separation science and technology Ročník 49; číslo 18; s. 2907 - 2912
Hlavní autoři: Patel, Jalpa, Desai, Gaurav, Upadhya, Archana
Médium: Journal Article
Jazyk:angličtina
Vydáno: Taylor & Francis 12.12.2014
Témata:
Adsorbents
antibiotic
Antibiotics
Chromatography
Elution
High performance liquid chromatography
Liquid chromatography
mupirocin
Pseudomonas fluorescens
Separation
ISSN:0149-6395, 1520-5754
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Abstract Mupirocin is an antibiotic from Pseudomonas fluorescens used topically against Staphylococci infections. There are many patented strategies which are proposed for separation of the antibiotic from the fermentation broth comprising of liquid-liquid extraction and hydrophobic chromatography. The paper demonstrates the use of native silica, as used in flash chromatography, as adsorbent for normal phase liquid chromatography of mupirocin. Mobile phase for elution was acidified n-hexane: ethyl acetate (10/90 v/v). The method achieved a yield of 66% relative to the mupirocin source and a concentration factor of 13. Anti-microbial assays, high performance liquid chromatography and Fourier transform infrared spectroscopy has been used to confirm the identity of the antibiotic after chromatographic separation. An alternative route to isolation of mupirocin using the polar sorbent has been suggested.
AbstractList Mupirocin is an antibiotic from Pseudomonas fluorescens used topically against Staphylococci infections. There are many patented strategies which are proposed for separation of the antibiotic from the fermentation broth comprising of liquid-liquid extraction and hydrophobic chromatography. The paper demonstrates the use of native silica, as used in flash chromatography, as adsorbent for normal phase liquid chromatography of mupirocin. Mobile phase for elution was acidified n-hexane: ethyl acetate (10/90 v/v). The method achieved a yield of 66% relative to the mupirocin source and a concentration factor of 13. Anti-microbial assays, high performance liquid chromatography and Fourier transform infrared spectroscopy has been used to confirm the identity of the antibiotic after chromatographic separation. An alternative route to isolation of mupirocin using the polar sorbent has been suggested.
Author Desai, Gaurav
Upadhya, Archana
Patel, Jalpa
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Cites_doi 10.1038/nrmicro2278
10.1016/j.cep.2004.05.006
10.1042/bj1760305
10.1074/jbc.273.48.31691
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Snippet Mupirocin is an antibiotic from Pseudomonas fluorescens used topically against Staphylococci infections. There are many patented strategies which are proposed...
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SubjectTerms Adsorbents
antibiotic
Antibiotics
Chromatography
Elution
High performance liquid chromatography
Liquid chromatography
mupirocin
Pseudomonas fluorescens
Separation
Title Separation of Mupirocin by Normal Phase Liquid Chromatography (NPLC)
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