Saliva-based point-of-care assay to measure the concentration of pyrazinamide using a mobile UV spectrophotometer

Pyrazinamide, one of the first-line antituberculosis drugs, displays variability in drug exposure that is associated with treatment response. A simple, low-cost assay may be helpful to optimize treatment. This study aimed to develop and validate a point-of-care assay to quantify the concentration of...

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Vydáno v:Journal of antimicrobial chemotherapy Ročník 80; číslo 1; s. 254
Hlavní autoři: Chen, Ricky Hao, Nguyen, Thi Anh, Kim, Hannah Yejin, Stocker, Sophie L, Alffenaar, Jan-Willem C
Médium: Journal Article
Jazyk:angličtina
Vydáno: England 03.01.2025
Témata:
Adult
Antitubercular Agents - analysis
Drug Monitoring - methods
Female
Humans
Male
Point-of-Care Systems
Pyrazinamide - analysis
Reproducibility of Results
Saliva - chemistry
Sensitivity and Specificity
Spectrophotometry, Ultraviolet - methods
ISSN:1460-2091, 1460-2091
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Shrnutí:Pyrazinamide, one of the first-line antituberculosis drugs, displays variability in drug exposure that is associated with treatment response. A simple, low-cost assay may be helpful to optimize treatment. This study aimed to develop and validate a point-of-care assay to quantify the concentration of pyrazinamide in saliva. All measurements were conducted using the nano-volume drop function on the mobile ultraviolet (UV) spectrophotometer (NP80, Implen, Germany). Assay development involved applying second derivative spectroscopy in combination with the Savitzky-Golay filter between wavelengths of 200-300 nm to increase spectral resolution. Assay validation included assessing selectivity, linearity, accuracy, precision, carry-over and matrix effects. Specificity was also analysed by evaluating the impact of co-administered medications on pyrazinamide results. Sample stability was measured at various temperatures up to 40°C. The calibration curve (7.5-200 mg/L) was linear (R2 = 0.9991). The overall accuracy (bias%) and precision (CV%) ranged from -0.66% to 5.15%, and 0.56% to 4.95%, respectively. Carry-over and matrix effects were both acceptable with a bias% of <±4% and CV% of <7.5%. Commonly co-administered medications displayed negligible interferences. Levofloxacin displayed analytical interference (bias% = -10.21%) at pyrazinamide concentrations < 25 mg/L, but this will have little clinical implications. Pyrazinamide was considered stable in saliva after 7 days in all storage conditions with a CV% of <6.5% and bias% of <±10.5% for both low- and high-quality control concentrations. A saliva-based assay for pyrazinamide has been successfully developed and validated using the mobile UV spectrophotometer.
Bibliografie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1460-2091
1460-2091
DOI:10.1093/jac/dkae404