Phytochemical Profiling of Ephedra alata subsp. alenda Seeds by High-Performance Liquid Chromatography-Electrospray Ionization-Quadrupole-Time-of-Flight-Mass Spectrometry (HPLC-ESI-QTOF-MS), Molecular Docking, and Antioxidant, Anti-diabetic, and Acetylcholinesterase Inhibition

The aim of the current work was to study for the first time the phytochemical profile of Ephedra alata subsp. alenda seeds extract (ESS). The extract was characterized using high-performance liquid chromatography coupled to electrospray ionization quadropole time-of-flight mass spectrometry (HPLC-ES...

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Vydané v:Analytical letters Ročník 55; číslo 15; s. 2450 - 2466
Hlavní autori: Mufti, Afoua, Tir, Meriam, Zarei, Armin, Contreras, María del Mar, Gómez-Cruz, Irene, Feriani, Anouar, Ghazouani, Lakhdar, Saadaoui, Ezzeddine, Allagui, Mohamed Salah, Harrath, Abdel Halim, Ramazani, Ali, Tlili, Nizar
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: Abingdon Taylor & Francis 13.10.2022
Taylor & Francis Ltd
Predmet:
Amylases
Antioxidant
Antioxidants
Biological activity
Chromatography
Diabetes
Electrospraying
enzymes
Ephedra alata subsp. alenda
Ephedrine
Fatty acids
Flavonols
Glucosidase
High-performance liquid chromatography
high-performance liquid chromatography-electrospray ionization-quadrupole-time-of-flight-mass spectrometry (HPLC-ESI-QTOF/MS)
Hydrogen bonds
Inhibitors
Ionization
Ions
Mass spectrometry
Molecular docking
Phytochemicals
Quadrupoles
Scientific imaging
Spectroscopy
ISSN:0003-2719, 1532-236X
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Shrnutí:The aim of the current work was to study for the first time the phytochemical profile of Ephedra alata subsp. alenda seeds extract (ESS). The extract was characterized using high-performance liquid chromatography coupled to electrospray ionization quadropole time-of-flight mass spectrometry (HPLC-ESI-QTOF/MS). The antioxidant and antihemolytic properties were evaluated, and the inhibitory properties against α-amylase, α-glucosidase, and acetylcholinesterase were assessed. Chromatographic analysis revealed that ESS was rich in bioactive components including ephedrine derivatives, sphingolipides, flavonol, hexoside, and fatty acids. Furthermore, ESS displayed important antioxidant activity and α-glucosidase, α-amylase, and acetylcholinesterase inhibitory capacities. Moreover, ESS showed a preventive effect against oxidative stress induced hemolysis. In silico analysis confirmed the beneficial effects of ESS-phytochemicals. In fact, 3 out of the 14 investigated compounds, including isorhamnetin rutinoside, isoschaftoside, and kaempferol-3-rhamnoside, exhibited the best binding scores (less than −7) with two anti-diabetic targets. Isorhamnetin rutinoside also showed the highest number of hydrogen bonds with acetylcholinesterase highlighting its anti-cholieaterase potency. The current study suggested that seeds from E. alata are interesting sources of bioactive compounds and recommended as enzyme inhibitory agents in food and pharmaceuticals.
Bibliografia:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 14
ISSN:0003-2719
1532-236X
DOI:10.1080/00032719.2022.2059082