A NEW FACILE METHOD FOR SPIN-LABELING OF OLIGONUCLEOTIDES

A new facile method for spin-labeling suitable for DNA and RNA oligonucleotides is presented. The nitroxide 3-ethenyl-2,2,5,5-tetramethyl-pyrrolin-1-yloxy was directly introduced during automated solid-phase synthesis by a Pd(0) cross coupling reaction. The main advantages of this procedure are the...

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Veröffentlicht in:Nucleosides, nucleotides & nucleic acids Jg. 20; H. 4-7; S. 1271 - 1274
Hauptverfasser: Strube, Thorsten, Schiemann, Olav, MacMillan, Fraser, Prisner, Thomas, Engels, Joachim W.
Format: Journal Article
Sprache:Englisch
Veröffentlicht: United States Taylor & Francis Group 01.04.2001
Schlagworte:
DNA - chemical synthesis
DNA - chemistry
Nucleic Acid Conformation
Oligonucleotides - chemical synthesis
Oligonucleotides - chemistry
RNA - chemical synthesis
RNA - chemistry
Spin Labels - chemical synthesis
ISSN:1525-7770, 1532-2335
Online-Zugang:Volltext
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Zusammenfassung:A new facile method for spin-labeling suitable for DNA and RNA oligonucleotides is presented. The nitroxide 3-ethenyl-2,2,5,5-tetramethyl-pyrrolin-1-yloxy was directly introduced during automated solid-phase synthesis by a Pd(0) cross coupling reaction. The main advantages of this procedure are the small amount of spin-label needed for the derivatisation of the oligonucleotide and the high coupling efficiency on the solid phase.
Bibliographie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:1525-7770
1532-2335
DOI:10.1081/NCN-100002534