UPLC-DAD-QToF/MS analysis of green tea phenolic metabolites in their free, esterified, glycosylated, and cell wall-bound forms by ultra-sonication, agitation, and conventional extraction techniques

In this study, phenolic metabolites in their free, soluble esterified, and soluble glycosylated forms in green tea infusions and cell wall-bound forms in solid residues obtained by ultrasound treatment, agitation treatment, and normal infusion preparation method were investigated. In total, thirty-t...

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Published in:Food science & technology Vol. 127; p. 109440
Main Authors: Das, Protiva Rani, Islam, Md. Tabibul, Lee, Seon-Hye, Lee, Min-Ki, Kim, Jung-Bong, Eun, Jong-Bang
Format: Journal Article
Language:English
Published: Elsevier Ltd 01.06.2020
Subjects:
agitation
Cell wall-bound phenolics
coumaric acids
epigallocatechin gallate
esterification
flavanols
Free phenolics
gallic acid
glycosylation
green tea
Green tea infusions
kaempferol
metabolites
Soluble esterified phenolics
Soluble glycosylated phenolics
ultrasonic treatment
ultrasonics
Soluble esterified phenolics
Soluble glycosylated phenolics
Green tea infusions
Free phenolics
Cell wall-bound phenolics
ISSN:0023-6438, 1096-1127
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Summary:In this study, phenolic metabolites in their free, soluble esterified, and soluble glycosylated forms in green tea infusions and cell wall-bound forms in solid residues obtained by ultrasound treatment, agitation treatment, and normal infusion preparation method were investigated. In total, thirty-three phenolic compounds were characterized including twenty-two flavonoids and eleven phenolic acids using UPLC-DAD-QToF/MS analysis. The flavonoids were sub-characterized as flavanols and flavonols, and phenolic acids were sub-characterized as hydroxybenzoic acid, hydroxybenzoylquinic acid, hydroxycinnamic acid, and hydroxycinnamoylquinic acid. As flavanols, EGCG was dominant in their free form and was significantly higher (p < 0.05) in ultrasound (4094 mg/100 g) and agitation (3906 mg/100 g) treatment than normal infusion (1657 mg/100 g). Kaempferol was predominant flavonols in their free form and was significantly higher (p < 0.05) in ultrasound (54.78 mg/100 g) and agitation (53.87 mg/100 g) treatment than normal infusion (30.67 mg/100 g). In contrary, cell wall-bound phenolics were dominated by gallic acid and were significantly higher (p < 0.05) in normal solid residues (2582 mg/100 g) than agitation (527 mg/100 g) and ultrasound (157 mg/100 g) treatment. This study provides a useful database of different forms of phenolic metabolite profiling of aqueous green tea infusions and solid residues. •Characterize green tea phenolic metabolites using UPLC-DAD-QToF/MS analysis.•Ultrasonic and agitation treatment is best to extract each form of phenolics.•Extraction techniques could increase soluble phenolic yields in green tea infusion.
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ISSN:0023-6438
1096-1127
DOI:10.1016/j.lwt.2020.109440