It's Not Just About Speed: Single-Cell Tracking Reveals Changes in MSC Motility Associated with Replicative Senescence

Mesenchymal stem cells, cultured in 2D, are motile fibroblast-like cells with a well-developed actin cytoskeleton. In this study, we analyzed changes in cell motility during long-term cultivation accompanied by replicative senescence (RS) for DF2 and MSCWJ-1 cell lines derived from various sources a...

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Published in:Stem cell reviews and reports Vol. 21; no. 5; pp. 1186 - 1198
Main Authors: Lukacheva, Anastasiya V., Bogachev, Mikhail I., Musorina, Anastasiya S., Kriger, Darya V., Poljanskaya, Galina G., Bobkov, Danila E.
Format: Journal Article
Language:English
Published: New York Springer US 01.06.2025
Springer Nature B.V
Subjects:
Actin
Biomedical and Life Sciences
Biomedical Engineering and Bioengineering
Cell Biology
Cell culture
Cell growth
Cell Line
Cell lines
Cell Movement - drug effects
Cell Tracking - methods
Cellular biology
Cellular Senescence - drug effects
Cytoskeleton
Enzymes
Humans
Life Sciences
Lysophosphatidic acid
Lysophospholipids - pharmacology
Mesenchymal stem cells
Mesenchymal Stem Cells - cytology
Mesenchymal Stem Cells - drug effects
Mesenchymal Stem Cells - metabolism
Morphology
Motility
Regenerative Medicine/Tissue Engineering
Senescence
Signal transduction
Single-Cell Analysis - methods
Statistical analysis
Stem Cells
Umbilical cord
United States > US
Germany
Russia
Cell motility
Multivariate statistical analysis
NPBNs
LCFD
Replicative senescence
LPA
Actin cytoskeleton
Mesenchymal stem cells
ISSN:2629-3269, 2629-3277, 2629-3277
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Abstract Mesenchymal stem cells, cultured in 2D, are motile fibroblast-like cells with a well-developed actin cytoskeleton. In this study, we analyzed changes in cell motility during long-term cultivation accompanied by replicative senescence (RS) for DF2 and MSCWJ-1 cell lines derived from various sources and donors of different age under both normal and inflammatory conditions, the latter obtained by treatment with lysophosphatidic acid (LPA). Our results indicate that RS is associated with non-stationary alterations in the average migration speed: while the median speed derived from single-cell tracking is unaffected by the senescence stage, the average speed in young cells is enhanced due to the contribution of a subpopulation of fast-moving cells. The sensitivity of cell motility metrics to the impact of LPA varied depending on their origin, with the most pronounced effects observed during the initial passages. Using multivariate statistical analysis, we have shown explicitly that the common motility metrics (average and maximum speed, distance, sinuosity of trajectories, etc.) are associated with the passage, thus clearly reflecting senescence effect. Altogether, our results indicate that cell motility exhibits complex alterations with RS, with multiple metrics besides the average speed being affected and associated with their RS stage. Graphical Abstract
AbstractList Mesenchymal stem cells, cultured in 2D, are motile fibroblast-like cells with a well-developed actin cytoskeleton. In this study, we analyzed changes in cell motility during long-term cultivation accompanied by replicative senescence (RS) for DF2 and MSCWJ-1 cell lines derived from various sources and donors of different age under both normal and inflammatory conditions, the latter obtained by treatment with lysophosphatidic acid (LPA). Our results indicate that RS is associated with non-stationary alterations in the average migration speed: while the median speed derived from single-cell tracking is unaffected by the senescence stage, the average speed in young cells is enhanced due to the contribution of a subpopulation of fast-moving cells. The sensitivity of cell motility metrics to the impact of LPA varied depending on their origin, with the most pronounced effects observed during the initial passages. Using multivariate statistical analysis, we have shown explicitly that the common motility metrics (average and maximum speed, distance, sinuosity of trajectories, etc.) are associated with the passage, thus clearly reflecting senescence effect. Altogether, our results indicate that cell motility exhibits complex alterations with RS, with multiple metrics besides the average speed being affected and associated with their RS stage.Mesenchymal stem cells, cultured in 2D, are motile fibroblast-like cells with a well-developed actin cytoskeleton. In this study, we analyzed changes in cell motility during long-term cultivation accompanied by replicative senescence (RS) for DF2 and MSCWJ-1 cell lines derived from various sources and donors of different age under both normal and inflammatory conditions, the latter obtained by treatment with lysophosphatidic acid (LPA). Our results indicate that RS is associated with non-stationary alterations in the average migration speed: while the median speed derived from single-cell tracking is unaffected by the senescence stage, the average speed in young cells is enhanced due to the contribution of a subpopulation of fast-moving cells. The sensitivity of cell motility metrics to the impact of LPA varied depending on their origin, with the most pronounced effects observed during the initial passages. Using multivariate statistical analysis, we have shown explicitly that the common motility metrics (average and maximum speed, distance, sinuosity of trajectories, etc.) are associated with the passage, thus clearly reflecting senescence effect. Altogether, our results indicate that cell motility exhibits complex alterations with RS, with multiple metrics besides the average speed being affected and associated with their RS stage.
Mesenchymal stem cells, cultured in 2D, are motile fibroblast-like cells with a well-developed actin cytoskeleton. In this study, we analyzed changes in cell motility during long-term cultivation accompanied by replicative senescence (RS) for DF2 and MSCWJ-1 cell lines derived from various sources and donors of different age under both normal and inflammatory conditions, the latter obtained by treatment with lysophosphatidic acid (LPA). Our results indicate that RS is associated with non-stationary alterations in the average migration speed: while the median speed derived from single-cell tracking is unaffected by the senescence stage, the average speed in young cells is enhanced due to the contribution of a subpopulation of fast-moving cells. The sensitivity of cell motility metrics to the impact of LPA varied depending on their origin, with the most pronounced effects observed during the initial passages. Using multivariate statistical analysis, we have shown explicitly that the common motility metrics (average and maximum speed, distance, sinuosity of trajectories, etc.) are associated with the passage, thus clearly reflecting senescence effect. Altogether, our results indicate that cell motility exhibits complex alterations with RS, with multiple metrics besides the average speed being affected and associated with their RS stage.
Mesenchymal stem cells, cultured in 2D, are motile fibroblast-like cells with a well-developed actin cytoskeleton. In this study, we analyzed changes in cell motility during long-term cultivation accompanied by replicative senescence (RS) for DF2 and MSCWJ-1 cell lines derived from various sources and donors of different age under both normal and inflammatory conditions, the latter obtained by treatment with lysophosphatidic acid (LPA). Our results indicate that RS is associated with non-stationary alterations in the average migration speed: while the median speed derived from single-cell tracking is unaffected by the senescence stage, the average speed in young cells is enhanced due to the contribution of a subpopulation of fast-moving cells. The sensitivity of cell motility metrics to the impact of LPA varied depending on their origin, with the most pronounced effects observed during the initial passages. Using multivariate statistical analysis, we have shown explicitly that the common motility metrics (average and maximum speed, distance, sinuosity of trajectories, etc.) are associated with the passage, thus clearly reflecting senescence effect. Altogether, our results indicate that cell motility exhibits complex alterations with RS, with multiple metrics besides the average speed being affected and associated with their RS stage. Graphical Abstract
Author Bobkov, Danila E.
Musorina, Anastasiya S.
Poljanskaya, Galina G.
Lukacheva, Anastasiya V.
Bogachev, Mikhail I.
Kriger, Darya V.
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Issue 5
Keywords Cell motility
Multivariate statistical analysis
NPBNs
LCFD
Replicative senescence
LPA
Actin cytoskeleton
Mesenchymal stem cells
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Snippet Mesenchymal stem cells, cultured in 2D, are motile fibroblast-like cells with a well-developed actin cytoskeleton. In this study, we analyzed changes in cell...
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SubjectTerms Actin
Biomedical and Life Sciences
Biomedical Engineering and Bioengineering
Cell Biology
Cell culture
Cell growth
Cell Line
Cell lines
Cell Movement - drug effects
Cell Tracking - methods
Cellular biology
Cellular Senescence - drug effects
Cytoskeleton
Enzymes
Humans
Life Sciences
Lysophosphatidic acid
Lysophospholipids - pharmacology
Mesenchymal stem cells
Mesenchymal Stem Cells - cytology
Mesenchymal Stem Cells - drug effects
Mesenchymal Stem Cells - metabolism
Morphology
Motility
Regenerative Medicine/Tissue Engineering
Senescence
Signal transduction
Single-Cell Analysis - methods
Statistical analysis
Stem Cells
Umbilical cord
Title It's Not Just About Speed: Single-Cell Tracking Reveals Changes in MSC Motility Associated with Replicative Senescence
URI https://link.springer.com/article/10.1007/s12015-025-10868-x
https://www.ncbi.nlm.nih.gov/pubmed/40156639
https://www.proquest.com/docview/3235513272
https://www.proquest.com/docview/3184018651
Volume 21
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