Synthesis of PDA-Mediated Magnetic Bimetallic Nanozyme and Its Application in Immunochromatographic Assay

Immunochromatographic assay (ICA) is widely applied in various fields. However, severe matrix interference and weak signal output present major challenges in achieving accurate and ultrasensitive detection in ICA. Here, a polydopamine (PDA)-mediated magnetic bimetallic nanozyme (Fe O @PDA@Pd/Pt) wit...

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Published in:ACS applied materials & interfaces Vol. 13; no. 1; p. 1413
Main Authors: Lai, Xiaocui, Zhang, Ganggang, Zeng, Lifeng, Xiao, Xiaoyue, Peng, Juan, Guo, Ping, Zhang, Wei, Lai, Weihua
Format: Journal Article
Language:English
Published: United States 13.01.2021
Subjects:
Animals
Antibodies, Monoclonal - immunology
Benzidines - chemistry
Catalysis
Chorionic Gonadotropin - blood
Chorionic Gonadotropin - immunology
Chromogenic Compounds - chemistry
Escherichia coli O157 - immunology
Escherichia coli O157 - isolation & purification
Food Contamination - analysis
Immunoassay - methods
Indoles - chemistry
Limit of Detection
Magnetite Nanoparticles - chemistry
Milk - microbiology
Oxidation-Reduction
Palladium - chemistry
Platinum - chemistry
Polymers - chemistry
Fe3O4@PDA@Pd/Pt
peroxidase-like activity
human chorionic gonadotropin
immunochromatographic assay
Escherichia coli O157:H7
ISSN:1944-8252, 1944-8252
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Summary:Immunochromatographic assay (ICA) is widely applied in various fields. However, severe matrix interference and weak signal output present major challenges in achieving accurate and ultrasensitive detection in ICA. Here, a polydopamine (PDA)-mediated magnetic bimetallic nanozyme (Fe O @PDA@Pd/Pt) with peroxidase-like activity was synthesized and used as a probe in ICA. The magnetic property of Fe O @PDA@Pd/Pt enabled effective magnetic enrichment of targets, thereby reducing the matrix interference in the sample. PDA coating on the magnetic bimetallic nanozyme was employed as a mediator and a stabilizer. It improved the catalytic ability and stability of the magnetic bimetallic nanozyme by providing more coordination sites for Pd/Pt growth and functional groups (-NH and -OH). In addition, the Pd/Pt bimetallic synergistic effect could further enhance the catalytic ability of the nanozyme. A method was developed by integrating Fe O , PDA, and Pd/Pt into Fe O @PDA@Pd/Pt as a probe in ICA. With the proposed method, human chorionic gonadotropin and O157:H7 were successfully detected to be as low as 0.0094 mIU/mL in human blood serum and 9 × 10 CFU/mL in the milk sample, respectively. This method may be readily adapted for accurate and ultrasensitive detection of other biomolecules in various fields.
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ISSN:1944-8252
1944-8252
DOI:10.1021/acsami.0c17957