Development and validation of a chiral LC-MS/MS method for the separation and quantification of four synthetic cathinones in human whole blood and its application in stability analysis

Synthetic cathinones, a subclass of new psychoactive substances, have gained high popularity on the recreational drugs market over the past years. These drugs typically have a chiral center, so they may exist as two stereoisomers. Therefore the pharmacological, pharmacokinetic or metabolic propertie...

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Published in:	Talanta (Oxford) Vol. 253; p. 123986
Main Authors:	Aldubayyan, Abdulaziz A., Castrignanò, Erika, Elliott, Simon, Abbate, Vincenzo
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier B.V 01.02.2023
Subjects:	Chiral LC-MS/MS Stability Synthetic cathinones Whole blood Whole blood Chiral LC-MS/MS Synthetic cathinones Stability
ISSN:	0039-9140, 1873-3573, 1873-3573
Online Access:	Get full text
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Abstract	Synthetic cathinones, a subclass of new psychoactive substances, have gained high popularity on the recreational drugs market over the past years. These drugs typically have a chiral center, so they may exist as two stereoisomers. Therefore the pharmacological, pharmacokinetic or metabolic properties of their enantiomers are expected to differ. However, these drugs are often synthesized and sold as a racemic mixture, and as a consequence, differentiation of their (R)- and (S)- enantiomers is relevant in clinical and forensic toxicology. Information about single enantiomers of synthetic cathinones is relatively scarce due to challenges of their chiral analysis. Hence, a sensitive and reliable liquid chromatography-tandem mass spectrometry method was developed and validated for the chiral separation and quantification of four synthetic cathinones in human whole blood samples. The method was fully validated in terms of linearity, limit of detection, limit of quantification, bias, precision, carryover, interferences, matrix effects, recovery and processed sample stability and successfully applied to evaluate the stability as well as enantioselective degradation of synthetic cathinones enantiomers under various storage conditions. For most of the analytes, significant enantioselective degradation was observed when stored at room temperature or refrigerated, with the E2-enantiomers observed to more rapidly degrade under both conditions. This is the first report concerning the stability and enantioselective degradation of synthetic cathinone enantiomers in whole blood. Moreover, the inversion study demonstrated enantiomeric inversion of R-(−)- and S-(+)-methylenedioxypyrovalerone (MDPV) in human whole blood and methanolic solution. [Display omitted] •A chiral LC-MS/MS method for selected synthetic cathinones was developed and validated.•The method was successfully employed to study stability of synthetic cathinone enantiomers in whole blood.•First report on enantiomeric inversion of MDPV.•This method would be useful for future pharmacokinetic studies.
AbstractList	Synthetic cathinones, a subclass of new psychoactive substances, have gained high popularity on the recreational drugs market over the past years. These drugs typically have a chiral center, so they may exist as two stereoisomers. Therefore the pharmacological, pharmacokinetic or metabolic properties of their enantiomers are expected to differ. However, these drugs are often synthesized and sold as a racemic mixture, and as a consequence, differentiation of their (R)- and (S)- enantiomers is relevant in clinical and forensic toxicology. Information about single enantiomers of synthetic cathinones is relatively scarce due to challenges of their chiral analysis. Hence, a sensitive and reliable liquid chromatography-tandem mass spectrometry method was developed and validated for the chiral separation and quantification of four synthetic cathinones in human whole blood samples. The method was fully validated in terms of linearity, limit of detection, limit of quantification, bias, precision, carryover, interferences, matrix effects, recovery and processed sample stability and successfully applied to evaluate the stability as well as enantioselective degradation of synthetic cathinones enantiomers under various storage conditions. For most of the analytes, significant enantioselective degradation was observed when stored at room temperature or refrigerated, with the E2-enantiomers observed to more rapidly degrade under both conditions. This is the first report concerning the stability and enantioselective degradation of synthetic cathinone enantiomers in whole blood. Moreover, the inversion study demonstrated enantiomeric inversion of R-(−)- and S-(+)-methylenedioxypyrovalerone (MDPV) in human whole blood and methanolic solution. [Display omitted] •A chiral LC-MS/MS method for selected synthetic cathinones was developed and validated.•The method was successfully employed to study stability of synthetic cathinone enantiomers in whole blood.•First report on enantiomeric inversion of MDPV.•This method would be useful for future pharmacokinetic studies. Synthetic cathinones, a subclass of new psychoactive substances, have gained high popularity on the recreational drugs market over the past years. These drugs typically have a chiral center, so they may exist as two stereoisomers. Therefore the pharmacological, pharmacokinetic or metabolic properties of their enantiomers are expected to differ. However, these drugs are often synthesized and sold as a racemic mixture, and as a consequence, differentiation of their (R)- and (S)- enantiomers is relevant in clinical and forensic toxicology. Information about single enantiomers of synthetic cathinones is relatively scarce due to challenges of their chiral analysis. Hence, a sensitive and reliable liquid chromatography-tandem mass spectrometry method was developed and validated for the chiral separation and quantification of four synthetic cathinones in human whole blood samples. The method was fully validated in terms of linearity, limit of detection, limit of quantification, bias, precision, carryover, interferences, matrix effects, recovery and processed sample stability and successfully applied to evaluate the stability as well as enantioselective degradation of synthetic cathinones enantiomers under various storage conditions. For most of the analytes, significant enantioselective degradation was observed when stored at room temperature or refrigerated, with the E2-enantiomers observed to more rapidly degrade under both conditions. This is the first report concerning the stability and enantioselective degradation of synthetic cathinone enantiomers in whole blood. Moreover, the inversion study demonstrated enantiomeric inversion of R-(-)- and S-(+)-methylenedioxypyrovalerone (MDPV) in human whole blood and methanolic solution.Synthetic cathinones, a subclass of new psychoactive substances, have gained high popularity on the recreational drugs market over the past years. These drugs typically have a chiral center, so they may exist as two stereoisomers. Therefore the pharmacological, pharmacokinetic or metabolic properties of their enantiomers are expected to differ. However, these drugs are often synthesized and sold as a racemic mixture, and as a consequence, differentiation of their (R)- and (S)- enantiomers is relevant in clinical and forensic toxicology. Information about single enantiomers of synthetic cathinones is relatively scarce due to challenges of their chiral analysis. Hence, a sensitive and reliable liquid chromatography-tandem mass spectrometry method was developed and validated for the chiral separation and quantification of four synthetic cathinones in human whole blood samples. The method was fully validated in terms of linearity, limit of detection, limit of quantification, bias, precision, carryover, interferences, matrix effects, recovery and processed sample stability and successfully applied to evaluate the stability as well as enantioselective degradation of synthetic cathinones enantiomers under various storage conditions. For most of the analytes, significant enantioselective degradation was observed when stored at room temperature or refrigerated, with the E2-enantiomers observed to more rapidly degrade under both conditions. This is the first report concerning the stability and enantioselective degradation of synthetic cathinone enantiomers in whole blood. Moreover, the inversion study demonstrated enantiomeric inversion of R-(-)- and S-(+)-methylenedioxypyrovalerone (MDPV) in human whole blood and methanolic solution. Synthetic cathinones, a subclass of new psychoactive substances, have gained high popularity on the recreational drugs market over the past years. These drugs typically have a chiral center, so they may exist as two stereoisomers. Therefore the pharmacological, pharmacokinetic or metabolic properties of their enantiomers are expected to differ. However, these drugs are often synthesized and sold as a racemic mixture, and as a consequence, differentiation of their (R)- and (S)- enantiomers is relevant in clinical and forensic toxicology. Information about single enantiomers of synthetic cathinones is relatively scarce due to challenges of their chiral analysis. Hence, a sensitive and reliable liquid chromatography-tandem mass spectrometry method was developed and validated for the chiral separation and quantification of four synthetic cathinones in human whole blood samples. The method was fully validated in terms of linearity, limit of detection, limit of quantification, bias, precision, carryover, interferences, matrix effects, recovery and processed sample stability and successfully applied to evaluate the stability as well as enantioselective degradation of synthetic cathinones enantiomers under various storage conditions. For most of the analytes, significant enantioselective degradation was observed when stored at room temperature or refrigerated, with the E2-enantiomers observed to more rapidly degrade under both conditions. This is the first report concerning the stability and enantioselective degradation of synthetic cathinone enantiomers in whole blood. Moreover, the inversion study demonstrated enantiomeric inversion of R-(-)- and S-(+)-methylenedioxypyrovalerone (MDPV) in human whole blood and methanolic solution.
ArticleNumber	123986
Author	Aldubayyan, Abdulaziz A. Castrignanò, Erika Abbate, Vincenzo Elliott, Simon
Author_xml	– sequence: 1 givenname: Abdulaziz A. orcidid: 0000-0002-5066-6615 surname: Aldubayyan fullname: Aldubayyan, Abdulaziz A. organization: Department of Analytical, Environmental & Forensic Sciences, Faculty of Life Sciences & Medicine, King's College London, London, UK – sequence: 2 givenname: Erika surname: Castrignanò fullname: Castrignanò, Erika organization: Department of Analytical, Environmental & Forensic Sciences, Faculty of Life Sciences & Medicine, King's College London, London, UK – sequence: 3 givenname: Simon orcidid: 0000-0001-5219-1687 surname: Elliott fullname: Elliott, Simon organization: Department of Analytical, Environmental & Forensic Sciences, Faculty of Life Sciences & Medicine, King's College London, London, UK – sequence: 4 givenname: Vincenzo orcidid: 0000-0002-3300-0520 surname: Abbate fullname: Abbate, Vincenzo email: vincenzo.abbate@kcl.ac.uk organization: Department of Analytical, Environmental & Forensic Sciences, Faculty of Life Sciences & Medicine, King's College London, London, UK
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Cites_doi	10.1124/jpet.115.229500 10.1016/j.forsciint.2020.110528 10.3389/fchem.2020.597726 10.1002/dta.2525 10.1016/0091-3057(94)00348-3 10.3390/ph15050510 10.1002/dta.1576 10.1016/j.jpba.2015.12.007 10.1016/j.chroma.2016.02.015 10.1002/dta.1686 10.1016/j.chroma.2010.08.075 10.1016/j.chroma.2016.12.064 10.3390/molecules27072057 10.1093/jat/bkx071 10.1016/j.jpba.2017.01.057 10.1093/jat/bks138 10.1007/s00216-021-03182-1 10.1111/bph.12951 10.1016/j.trac.2021.116326 10.1093/jat/bkaa074 10.1016/j.chroma.2020.461359 10.1002/dta.2990 10.1097/FTD.0000000000000808 10.1016/j.forsciint.2015.07.021 10.1021/ac020361s 10.1021/acschemneuro.5b00006 10.1016/j.trac.2018.11.010 10.1016/j.forsciint.2018.12.001 10.1016/j.lfs.2013.09.026 10.2174/1389200043335360 10.1016/j.chroma.2012.07.045 10.1093/jat/bkx074
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Keywords	Whole blood Chiral LC-MS/MS Synthetic cathinones Stability
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References	Loganathan, Yi, Patel, Zhang, Kong (bib17) 2021; 413 Zuba, Adamowicz (bib29) 2018 Norstrom (bib23) 2000; 34 Lewin, Seltzman, Carroll, Mascarella, Reddy (bib38) 2014; 97 Gregg, Baumann, Partilla, Bonano, Vouga, Tallarida, Velvadapu, Smith, Peet, Reitz, Negus, Rawls (bib7) 2015; 172 Castrignanò, Mardal, Rydevik, Miserez, Ramsey, Shine, Panto, Meyer, Kasprzyk-Hordern (bib13) 2017; 7 Czerwinska, Parkin, Dargan, George, Kicman, Abbate (bib33) 2019; 11 Aldubayyan, Castrignanò, Elliott, Abbate (bib36) 2022 Soh, Elliott (bib28) 2014; 6 Silva, Fernandes, de Pinho, Remião (bib10) 2018; 42 Matuszewski, Constanzer, Chavez-Eng (bib22) 2003; 75 Czerwinska, Parkin, Cilibrizzi, George, Kicman, Dargan, Abbate (bib18) 2021; 14 Aldubayyan, Castrignanò, Elliott, Abbate (bib9) 2021; 13 Gannon, Williamson, Suzuki, Rice, Fantegrossi (bib41) 2016; 356 Kolanos, Partilla, Baumann, Hutsell, Banks, Negus, Glennon (bib40) 2015; 6 Zanda, Fattore (bib2) 2017 Albals, Vander Heyden, Schmid, Chankvetadze, Mangelings (bib5) 2016; 121 Schwaninger, Meyer, Maurer (bib8) 2012; 1269 Almeida, Silva, de Pinho, Remião, Fernandes (bib11) 2022; 27 Cirilli, Guglielmi, Formica, Casulli, Carradori (bib25) 2017; 139 (bib4) 2022 Barreiro, Tiritan, Cass (bib12) 2021; 142 Peng, Jayapalan, Chankvetadze, Farkas (bib24) 2010; 1217 Wsol, Skalova, Szotakova (bib39) 2005; 5 Petrie, Camacho Muñoz, Martín (bib27) 2019; 110 Brandt, King, Evans-Brown (bib3) 2014; 6 Ciallella, Rutter, Nisbet, Scott (bib37) 2020; 8 Aldubayyan, Castrignanò, Elliott, Abbate (bib20) 2022; 15 UNODC (bib1) 2020 Busardò, Kyriakou, Tittarelli, Mannocchi, Pantano, Santurro, Zaami, Baglìo (bib31) 2015; 256 Matarashvili, Ghughunishvili, Chankvetadze, Takaishvili, Khatiashvili, Tsintsadze, Farkas, Chankvetadze (bib26) 2017; 1483 Johnson, Botch-Jones (bib30) 2013; 37 Antunes, Sequeira, de Caires Pereira, Caldeira, Santos, Franco, Barroso, Gaspar (bib35) 2021; 45 American Academy of Forensic Science (bib21) 2019 Adamowicz, Malczyk (bib34) 2019; 295 Glennon, Young, Martin, Dal Cason (bib6) 1995; 50 Castrignanò, Lubben, Kasprzyk-Hordern (bib14) 2016; 1438 Glicksberg, Kerrigan (bib32) 2017; 41 La Maida, Di Trana, Giorgetti, Tagliabracci, Busardò, Huestis (bib19) 2021; 43 Lin, Kuo (bib15) 2020; 317 Fu, Borrull, Marcé, Fontanals (bib16) 2020; 1626 Almeida (10.1016/j.talanta.2022.123986_bib11) 2022; 27 Matarashvili (10.1016/j.talanta.2022.123986_bib26) 2017; 1483 Czerwinska (10.1016/j.talanta.2022.123986_bib33) 2019; 11 Ciallella (10.1016/j.talanta.2022.123986_bib37) 2020; 8 Matuszewski (10.1016/j.talanta.2022.123986_bib22) 2003; 75 Soh (10.1016/j.talanta.2022.123986_bib28) 2014; 6 American Academy of Forensic Science (10.1016/j.talanta.2022.123986_bib21) 2019 Castrignanò (10.1016/j.talanta.2022.123986_bib14) 2016; 1438 Peng (10.1016/j.talanta.2022.123986_bib24) 2010; 1217 Zanda (10.1016/j.talanta.2022.123986_bib2) 2017 Brandt (10.1016/j.talanta.2022.123986_bib3) 2014; 6 Aldubayyan (10.1016/j.talanta.2022.123986_bib9) 2021; 13 Cirilli (10.1016/j.talanta.2022.123986_bib25) 2017; 139 Busardò (10.1016/j.talanta.2022.123986_bib31) 2015; 256 Silva (10.1016/j.talanta.2022.123986_bib10) 2018; 42 Lin (10.1016/j.talanta.2022.123986_bib15) 2020; 317 Wsol (10.1016/j.talanta.2022.123986_bib39) 2005; 5 (10.1016/j.talanta.2022.123986_bib4) 2022 Gregg (10.1016/j.talanta.2022.123986_bib7) 2015; 172 Kolanos (10.1016/j.talanta.2022.123986_bib40) 2015; 6 Glennon (10.1016/j.talanta.2022.123986_bib6) 1995; 50 Albals (10.1016/j.talanta.2022.123986_bib5) 2016; 121 Zuba (10.1016/j.talanta.2022.123986_bib29) 2018 Aldubayyan (10.1016/j.talanta.2022.123986_bib36) 2022 Castrignanò (10.1016/j.talanta.2022.123986_bib13) 2017; 7 Lewin (10.1016/j.talanta.2022.123986_bib38) 2014; 97 Fu (10.1016/j.talanta.2022.123986_bib16) 2020; 1626 Johnson (10.1016/j.talanta.2022.123986_bib30) 2013; 37 Antunes (10.1016/j.talanta.2022.123986_bib35) 2021; 45 Schwaninger (10.1016/j.talanta.2022.123986_bib8) 2012; 1269 Glicksberg (10.1016/j.talanta.2022.123986_bib32) 2017; 41 Adamowicz (10.1016/j.talanta.2022.123986_bib34) 2019; 295 Norstrom (10.1016/j.talanta.2022.123986_bib23) 2000; 34 Aldubayyan (10.1016/j.talanta.2022.123986_bib20) 2022; 15 Loganathan (10.1016/j.talanta.2022.123986_bib17) 2021; 413 Barreiro (10.1016/j.talanta.2022.123986_bib12) 2021; 142 La Maida (10.1016/j.talanta.2022.123986_bib19) 2021; 43 Gannon (10.1016/j.talanta.2022.123986_bib41) 2016; 356 Czerwinska (10.1016/j.talanta.2022.123986_bib18) 2021; 14 Petrie (10.1016/j.talanta.2022.123986_bib27) 2019; 110 UNODC (10.1016/j.talanta.2022.123986_bib1) 2020
References_xml	– year: 2020 ident: bib1 – volume: 256 start-page: 28 year: 2015 end-page: 37 ident: bib31 article-title: Assessment of the stability of mephedrone in ante-mortem and post-mortem blood specimens publication-title: Forensic Sci. Int. – year: 2022 ident: bib36 article-title: Influence of Long - Term Storage Temperatures and Sodium Fluoride Preservation on the Stability of Synthetic Cathinones and Dihydro - Metabolites in Human Whole Blood – volume: 15 year: 2022 ident: bib20 article-title: A quantitative LC–MS/MS method for the detection of 16 synthetic cathinones and 10 metabolites and its application to suspicious clinical and forensic urine samples publication-title: Pharmaceuticals – volume: 172 start-page: 883 year: 2015 end-page: 894 ident: bib7 article-title: Stereochemistry of mephedrone neuropharmacology: enantiomer-specific behavioural and neurochemical effects in rats publication-title: Br. J. Pharmacol. – volume: 1438 start-page: 84 year: 2016 end-page: 99 ident: bib14 article-title: Enantiomeric profiling of chiral drug biomarkers in wastewater with the usage of chiral liquid chromatography coupled with tandem mass spectrometry publication-title: J. Chromatogr. A – volume: 43 start-page: 52 year: 2021 end-page: 68 ident: bib19 article-title: A review of synthetic cathinone-related fatalities from 2017 to 2020 publication-title: Ther. Drug Monit. – volume: 6 start-page: 587 year: 2014 end-page: 597 ident: bib3 article-title: The new drug phenomenon publication-title: Drug Test. Anal. – volume: 142 year: 2021 ident: bib12 article-title: Challenges and innovations in chiral drugs in an environmental and bioanalysis perspective publication-title: TrAC, Trends Anal. Chem. – volume: 121 start-page: 232 year: 2016 end-page: 243 ident: bib5 article-title: Chiral separations of cathinone and amphetamine-derivatives: comparative study between capillary electrochromatography, supercritical fluid chromatography and three liquid chromatographic modes publication-title: J. Pharm. Biomed. Anal. – volume: 75 start-page: 3019 year: 2003 end-page: 3030 ident: bib22 article-title: Strategies for the assessment of matrix effect in quantitative bioanalytical methods based on HPLC-MS/MS publication-title: Anal. Chem. – volume: 295 start-page: 36 year: 2019 end-page: 45 ident: bib34 article-title: Stability of synthetic cathinones in blood and urine publication-title: Forensic Sci. Int. – volume: 11 start-page: 586 year: 2019 end-page: 594 ident: bib33 article-title: Stability of mephedrone and five of its phase I metabolites in human whole blood publication-title: Drug Test. Anal. – volume: 8 start-page: 1 year: 2020 end-page: 11 ident: bib37 article-title: Extended stability evaluation of selected cathinones publication-title: Front. Chem. – year: 2017 ident: bib2 article-title: Novel Psychoactive Substances: A New Behavioral and Mental Health Threat – volume: 7 start-page: 1 year: 2017 end-page: 12 ident: bib13 article-title: A new approach towards biomarker selection in estimation of human exposure to chiral chemicals: a case study of mephedrone publication-title: Sci. Rep. – volume: 1626 year: 2020 ident: bib16 article-title: Enantiomeric determination of cathinones in environmental water samples by liquid chromatography-high resolution mass spectrometry publication-title: J. Chromatogr. A – volume: 110 start-page: 249 year: 2019 end-page: 258 ident: bib27 article-title: Stereoselective LC–MS/MS methodologies for environmental analysis of chiral pesticides publication-title: TrAC, Trends Anal. Chem. – volume: 1269 start-page: 122 year: 2012 end-page: 135 ident: bib8 article-title: Chiral drug analysis using mass spectrometric detection relevant to research and practice in clinical and forensic toxicology publication-title: J. Chromatogr. A – volume: 13 start-page: 44 year: 2021 end-page: 68 ident: bib9 article-title: Stability of synthetic cathinones in clinical and forensic toxicological analysis—where are we now? publication-title: Drug Test. Anal. – volume: 139 start-page: 1 year: 2017 end-page: 7 ident: bib25 article-title: The sodium salt of the enantiomers of ricobendazole: preparation, solubility and chiroptical properties publication-title: J. Pharm. Biomed. Anal. – volume: 34 start-page: 29 year: 2000 end-page: 31 ident: bib23 article-title: Enantiomer fractions are preferred to enantiomer ratios for describing chiral signatures in publication-title: Environmental Analysis – year: 2022 ident: bib4 publication-title: Trends and Developments – volume: 45 start-page: 233 year: 2021 end-page: 242 ident: bib35 article-title: Determination of selected cathinones in blood by solid-phase extraction and GC-MS publication-title: J. Anal. Toxicol. – volume: 41 start-page: 711 year: 2017 end-page: 719 ident: bib32 article-title: Stability of synthetic cathinones in blood publication-title: J. Anal. Toxicol. – volume: 14 start-page: 1 year: 2021 end-page: 10 ident: bib18 article-title: Pharmacokinetics of mephedrone enantiomers in whole blood after a controlled intranasal administration to healthy human volunteers publication-title: Pharmaceuticals – volume: 6 start-page: 696 year: 2014 end-page: 704 ident: bib28 article-title: An investigation of the stability of emerging new psychoactive substances publication-title: Drug Test. Anal. – volume: 5 start-page: 517 year: 2005 end-page: 533 ident: bib39 article-title: Chiral inversion of drugs: coincidence or principle? publication-title: Curr. Drug Metabol. – volume: 27 start-page: 2057 year: 2022 ident: bib11 article-title: Synthetic cathinones: recent developments, enantioselectivity studies and enantioseparation methods publication-title: Molecules – volume: 1217 start-page: 6942 year: 2010 end-page: 6955 ident: bib24 article-title: Reversed-phase chiral HPLC and LC/MS analysis with tris(chloromethylphenylcarbamate) derivatives of cellulose and amylose as chiral stationary phases publication-title: J. Chromatogr. A – volume: 413 start-page: 2147 year: 2021 end-page: 2161 ident: bib17 article-title: A sensitive HPLC-MS/MS method for the detection, resolution and quantitation of cathinone enantiomers in horse blood plasma and urine publication-title: Anal. Bioanal. Chem. – volume: 37 start-page: 51 year: 2013 end-page: 55 ident: bib30 article-title: The stability of four designer drugs: MDPV, mephedrone, BZP and TFMPP in three biological matrices under various storage conditions publication-title: J. Anal. Toxicol. – volume: 6 start-page: 771 year: 2015 end-page: 777 ident: bib40 article-title: Stereoselective actions of methylenedioxypyrovalerone (MDPV) to inhibit dopamine and norepinephrine transporters and facilitate intracranial self-stimulation in rats publication-title: ACS Chem. Neurosci. – volume: 317 year: 2020 ident: bib15 article-title: Determination of the R- and S-enantiomers of methylone and ethylone in seized drugs by enantioselective liquid chromatography tandem mass spectrometry analysis publication-title: Forensic Sci. Int. – volume: 97 start-page: 9 year: 2014 end-page: 19 ident: bib38 article-title: Emergence and properties of spice and bath salts: a medicinal chemistry perspective publication-title: Life Sci. – volume: 50 start-page: 601 year: 1995 end-page: 606 ident: bib6 article-title: Methcathinone (“cat”): an enantiomeric potency comparison publication-title: Pharmacol. Biochem. Behav. – volume: 42 start-page: 17 year: 2018 end-page: 24 ident: bib10 article-title: Chiral resolution and enantioselectivity of synthetic cathinones: a brief review publication-title: J. Anal. Toxicol. – volume: 1483 start-page: 86 year: 2017 end-page: 92 ident: bib26 article-title: Separation of enantiomers of chiral weak acids with polysaccharide-based chiral columns and aqueous-organic mobile phases in high-performance liquid chromatography: typical reversed-phase behavior? publication-title: J. Chromatogr. A – volume: 356 start-page: 615 year: 2016 end-page: 623 ident: bib41 article-title: Stereoselective effects of abused “bath salt” constituent 3,4-methylenedioxypyrovalerone in mice: drug discrimination, locomotor activity, and thermoregulation publication-title: J. Pharmacol. Exp. Therapeut. – start-page: 1 year: 2019 end-page: 40 ident: bib21 article-title: Standard Practices for Method Validation in Forensic Toxicology – year: 2018 ident: bib29 article-title: Analytical Methods Used for Identification and Determination of Synthetic Cathinones and Their Metabolites – year: 2022 ident: 10.1016/j.talanta.2022.123986_bib36 – volume: 356 start-page: 615 year: 2016 ident: 10.1016/j.talanta.2022.123986_bib41 article-title: Stereoselective effects of abused “bath salt” constituent 3,4-methylenedioxypyrovalerone in mice: drug discrimination, locomotor activity, and thermoregulation publication-title: J. Pharmacol. Exp. Therapeut. doi: 10.1124/jpet.115.229500 – volume: 317 year: 2020 ident: 10.1016/j.talanta.2022.123986_bib15 article-title: Determination of the R- and S-enantiomers of methylone and ethylone in seized drugs by enantioselective liquid chromatography tandem mass spectrometry analysis publication-title: Forensic Sci. Int. doi: 10.1016/j.forsciint.2020.110528 – volume: 8 start-page: 1 year: 2020 ident: 10.1016/j.talanta.2022.123986_bib37 article-title: Extended stability evaluation of selected cathinones publication-title: Front. Chem. doi: 10.3389/fchem.2020.597726 – volume: 11 start-page: 586 year: 2019 ident: 10.1016/j.talanta.2022.123986_bib33 article-title: Stability of mephedrone and five of its phase I metabolites in human whole blood publication-title: Drug Test. Anal. doi: 10.1002/dta.2525 – volume: 50 start-page: 601 year: 1995 ident: 10.1016/j.talanta.2022.123986_bib6 article-title: Methcathinone (“cat”): an enantiomeric potency comparison publication-title: Pharmacol. Biochem. Behav. doi: 10.1016/0091-3057(94)00348-3 – volume: 7 start-page: 1 year: 2017 ident: 10.1016/j.talanta.2022.123986_bib13 article-title: A new approach towards biomarker selection in estimation of human exposure to chiral chemicals: a case study of mephedrone publication-title: Sci. Rep. – volume: 15 year: 2022 ident: 10.1016/j.talanta.2022.123986_bib20 article-title: A quantitative LC–MS/MS method for the detection of 16 synthetic cathinones and 10 metabolites and its application to suspicious clinical and forensic urine samples publication-title: Pharmaceuticals doi: 10.3390/ph15050510 – volume: 14 start-page: 1 year: 2021 ident: 10.1016/j.talanta.2022.123986_bib18 article-title: Pharmacokinetics of mephedrone enantiomers in whole blood after a controlled intranasal administration to healthy human volunteers publication-title: Pharmaceuticals – volume: 6 start-page: 696 year: 2014 ident: 10.1016/j.talanta.2022.123986_bib28 article-title: An investigation of the stability of emerging new psychoactive substances publication-title: Drug Test. Anal. doi: 10.1002/dta.1576 – volume: 121 start-page: 232 year: 2016 ident: 10.1016/j.talanta.2022.123986_bib5 article-title: Chiral separations of cathinone and amphetamine-derivatives: comparative study between capillary electrochromatography, supercritical fluid chromatography and three liquid chromatographic modes publication-title: J. Pharm. Biomed. Anal. doi: 10.1016/j.jpba.2015.12.007 – volume: 1438 start-page: 84 year: 2016 ident: 10.1016/j.talanta.2022.123986_bib14 article-title: Enantiomeric profiling of chiral drug biomarkers in wastewater with the usage of chiral liquid chromatography coupled with tandem mass spectrometry publication-title: J. Chromatogr. A doi: 10.1016/j.chroma.2016.02.015 – volume: 6 start-page: 587 year: 2014 ident: 10.1016/j.talanta.2022.123986_bib3 article-title: The new drug phenomenon publication-title: Drug Test. Anal. doi: 10.1002/dta.1686 – volume: 1217 start-page: 6942 year: 2010 ident: 10.1016/j.talanta.2022.123986_bib24 article-title: Reversed-phase chiral HPLC and LC/MS analysis with tris(chloromethylphenylcarbamate) derivatives of cellulose and amylose as chiral stationary phases publication-title: J. Chromatogr. A doi: 10.1016/j.chroma.2010.08.075 – volume: 1483 start-page: 86 year: 2017 ident: 10.1016/j.talanta.2022.123986_bib26 article-title: Separation of enantiomers of chiral weak acids with polysaccharide-based chiral columns and aqueous-organic mobile phases in high-performance liquid chromatography: typical reversed-phase behavior? publication-title: J. Chromatogr. A doi: 10.1016/j.chroma.2016.12.064 – volume: 27 start-page: 2057 year: 2022 ident: 10.1016/j.talanta.2022.123986_bib11 article-title: Synthetic cathinones: recent developments, enantioselectivity studies and enantioseparation methods publication-title: Molecules doi: 10.3390/molecules27072057 – year: 2018 ident: 10.1016/j.talanta.2022.123986_bib29 – volume: 41 start-page: 711 year: 2017 ident: 10.1016/j.talanta.2022.123986_bib32 article-title: Stability of synthetic cathinones in blood publication-title: J. Anal. Toxicol. doi: 10.1093/jat/bkx071 – start-page: 1 year: 2019 ident: 10.1016/j.talanta.2022.123986_bib21 – volume: 139 start-page: 1 year: 2017 ident: 10.1016/j.talanta.2022.123986_bib25 article-title: The sodium salt of the enantiomers of ricobendazole: preparation, solubility and chiroptical properties publication-title: J. Pharm. Biomed. Anal. doi: 10.1016/j.jpba.2017.01.057 – volume: 37 start-page: 51 year: 2013 ident: 10.1016/j.talanta.2022.123986_bib30 article-title: The stability of four designer drugs: MDPV, mephedrone, BZP and TFMPP in three biological matrices under various storage conditions publication-title: J. Anal. Toxicol. doi: 10.1093/jat/bks138 – volume: 413 start-page: 2147 year: 2021 ident: 10.1016/j.talanta.2022.123986_bib17 article-title: A sensitive HPLC-MS/MS method for the detection, resolution and quantitation of cathinone enantiomers in horse blood plasma and urine publication-title: Anal. Bioanal. Chem. doi: 10.1007/s00216-021-03182-1 – year: 2017 ident: 10.1016/j.talanta.2022.123986_bib2 – volume: 172 start-page: 883 year: 2015 ident: 10.1016/j.talanta.2022.123986_bib7 article-title: Stereochemistry of mephedrone neuropharmacology: enantiomer-specific behavioural and neurochemical effects in rats publication-title: Br. J. Pharmacol. doi: 10.1111/bph.12951 – volume: 142 year: 2021 ident: 10.1016/j.talanta.2022.123986_bib12 article-title: Challenges and innovations in chiral drugs in an environmental and bioanalysis perspective publication-title: TrAC, Trends Anal. Chem. doi: 10.1016/j.trac.2021.116326 – volume: 45 start-page: 233 year: 2021 ident: 10.1016/j.talanta.2022.123986_bib35 article-title: Determination of selected cathinones in blood by solid-phase extraction and GC-MS publication-title: J. Anal. Toxicol. doi: 10.1093/jat/bkaa074 – volume: 1626 year: 2020 ident: 10.1016/j.talanta.2022.123986_bib16 article-title: Enantiomeric determination of cathinones in environmental water samples by liquid chromatography-high resolution mass spectrometry publication-title: J. Chromatogr. A doi: 10.1016/j.chroma.2020.461359 – volume: 13 start-page: 44 year: 2021 ident: 10.1016/j.talanta.2022.123986_bib9 article-title: Stability of synthetic cathinones in clinical and forensic toxicological analysis—where are we now? publication-title: Drug Test. Anal. doi: 10.1002/dta.2990 – volume: 43 start-page: 52 year: 2021 ident: 10.1016/j.talanta.2022.123986_bib19 article-title: A review of synthetic cathinone-related fatalities from 2017 to 2020 publication-title: Ther. Drug Monit. doi: 10.1097/FTD.0000000000000808 – volume: 256 start-page: 28 year: 2015 ident: 10.1016/j.talanta.2022.123986_bib31 article-title: Assessment of the stability of mephedrone in ante-mortem and post-mortem blood specimens publication-title: Forensic Sci. Int. doi: 10.1016/j.forsciint.2015.07.021 – volume: 75 start-page: 3019 year: 2003 ident: 10.1016/j.talanta.2022.123986_bib22 article-title: Strategies for the assessment of matrix effect in quantitative bioanalytical methods based on HPLC-MS/MS publication-title: Anal. Chem. doi: 10.1021/ac020361s – volume: 6 start-page: 771 year: 2015 ident: 10.1016/j.talanta.2022.123986_bib40 article-title: Stereoselective actions of methylenedioxypyrovalerone (MDPV) to inhibit dopamine and norepinephrine transporters and facilitate intracranial self-stimulation in rats publication-title: ACS Chem. Neurosci. doi: 10.1021/acschemneuro.5b00006 – volume: 110 start-page: 249 year: 2019 ident: 10.1016/j.talanta.2022.123986_bib27 article-title: Stereoselective LC–MS/MS methodologies for environmental analysis of chiral pesticides publication-title: TrAC, Trends Anal. Chem. doi: 10.1016/j.trac.2018.11.010 – year: 2022 ident: 10.1016/j.talanta.2022.123986_bib4 publication-title: Trends and Developments – volume: 295 start-page: 36 year: 2019 ident: 10.1016/j.talanta.2022.123986_bib34 article-title: Stability of synthetic cathinones in blood and urine publication-title: Forensic Sci. Int. doi: 10.1016/j.forsciint.2018.12.001 – volume: 97 start-page: 9 year: 2014 ident: 10.1016/j.talanta.2022.123986_bib38 article-title: Emergence and properties of spice and bath salts: a medicinal chemistry perspective publication-title: Life Sci. doi: 10.1016/j.lfs.2013.09.026 – volume: 5 start-page: 517 year: 2005 ident: 10.1016/j.talanta.2022.123986_bib39 article-title: Chiral inversion of drugs: coincidence or principle? publication-title: Curr. Drug Metabol. doi: 10.2174/1389200043335360 – volume: 1269 start-page: 122 year: 2012 ident: 10.1016/j.talanta.2022.123986_bib8 article-title: Chiral drug analysis using mass spectrometric detection relevant to research and practice in clinical and forensic toxicology publication-title: J. Chromatogr. A doi: 10.1016/j.chroma.2012.07.045 – volume: 42 start-page: 17 year: 2018 ident: 10.1016/j.talanta.2022.123986_bib10 article-title: Chiral resolution and enantioselectivity of synthetic cathinones: a brief review publication-title: J. Anal. Toxicol. doi: 10.1093/jat/bkx074 – year: 2020 ident: 10.1016/j.talanta.2022.123986_bib1 – volume: 34 start-page: 29 year: 2000 ident: 10.1016/j.talanta.2022.123986_bib23 article-title: Enantiomer fractions are preferred to enantiomer ratios for describing chiral signatures in publication-title: Environmental Analysis
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Snippet	Synthetic cathinones, a subclass of new psychoactive substances, have gained high popularity on the recreational drugs market over the past years. These drugs...
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Title	Development and validation of a chiral LC-MS/MS method for the separation and quantification of four synthetic cathinones in human whole blood and its application in stability analysis
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