Genotype III-Based Japanese Encephalitis Vaccines Exhibit Diminished Neutralizing Response to Reemerging Genotype V

Japanese encephalitis (JE) has been predominantly controlled through vaccination. However, the isolation of JE virus (JEV) genotype V (GV) in China in 2009, and the subsequent alarming increase in JE cases in the Republic of Korea since 2010, present a new challenge. Serum samples from individuals v...

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Published in:The Journal of infectious diseases Vol. 231; no. 5; p. 1281
Main Authors: Lee, Ah-Ra, Kim, Woo-Jin, Choi, Haeyoun, Kim, Sang-Hyun, Hong, Su-Yeon, Shim, Sang-Mu, Lee, Hee Il, Song, Jae Min, Kim, Seong-Jun, Ishikawa, Tomohiro, Kang, Ji-Man, Eom, Hyeon-Seok, Seo, Sang-Uk
Format: Journal Article
Language:English
Published: United States 15.05.2025
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ISSN:1537-6613
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Abstract Japanese encephalitis (JE) has been predominantly controlled through vaccination. However, the isolation of JE virus (JEV) genotype V (GV) in China in 2009, and the subsequent alarming increase in JE cases in the Republic of Korea since 2010, present a new challenge. Serum samples from individuals vaccinated with genotype III (GIII)-based JE vaccines were analyzed for neutralizing seroresponse to GV isolates. Serum from immunocompromised pediatric patients vaccinated with an inactivated JE vaccine showed higher 50% plaque reduction neutralization test geometric mean titer (GMT) against GIII Nakayama (11 358; 95% confidence interval [CI], 1790-29 658), but lower GMTs against GV isolates: GV Muar (499; 95% CI, 0-2437), GV 43279 (308; 95% CI, 159-582), and GV 43413 (231; 95% CI, 108-738). Similarly, 32 healthy volunteers receiving a live attenuated JE vaccine achieved 100% seroprotection against GIII Nakayama with GMT of 338 (95% CI, 304-651) at 1 month postvaccination. However, GMTs against GV isolates were 123 (95% CI, 102-446) for GV Muar, 81 (95% CI, 63-168) for GV 43279, and 107 (95% CI, 100-322) for GV 43413, not achieving 100% seroprotection against these isolates. At 6 months postvaccination, GMT against Nakayama increased to 696 (95% CI, 409-2353), while remaining similar for GV isolates. Our study underscores that current GIII-based vaccines do not provide comparable protection against GV JEVs, impacting individuals in both current and potential endemic regions, as well as travelers to these regions.
AbstractList Japanese encephalitis (JE) has been predominantly controlled through vaccination. However, the isolation of JE virus (JEV) genotype V (GV) in China in 2009, and the subsequent alarming increase in JE cases in the Republic of Korea since 2010, present a new challenge. Serum samples from individuals vaccinated with genotype III (GIII)-based JE vaccines were analyzed for neutralizing seroresponse to GV isolates. Serum from immunocompromised pediatric patients vaccinated with an inactivated JE vaccine showed higher 50% plaque reduction neutralization test geometric mean titer (GMT) against GIII Nakayama (11 358; 95% confidence interval [CI], 1790-29 658), but lower GMTs against GV isolates: GV Muar (499; 95% CI, 0-2437), GV 43279 (308; 95% CI, 159-582), and GV 43413 (231; 95% CI, 108-738). Similarly, 32 healthy volunteers receiving a live attenuated JE vaccine achieved 100% seroprotection against GIII Nakayama with GMT of 338 (95% CI, 304-651) at 1 month postvaccination. However, GMTs against GV isolates were 123 (95% CI, 102-446) for GV Muar, 81 (95% CI, 63-168) for GV 43279, and 107 (95% CI, 100-322) for GV 43413, not achieving 100% seroprotection against these isolates. At 6 months postvaccination, GMT against Nakayama increased to 696 (95% CI, 409-2353), while remaining similar for GV isolates. Our study underscores that current GIII-based vaccines do not provide comparable protection against GV JEVs, impacting individuals in both current and potential endemic regions, as well as travelers to these regions.
Author Lee, Ah-Ra
Kim, Seong-Jun
Kang, Ji-Man
Hong, Su-Yeon
Eom, Hyeon-Seok
Kim, Sang-Hyun
Shim, Sang-Mu
Lee, Hee Il
Ishikawa, Tomohiro
Kim, Woo-Jin
Seo, Sang-Uk
Song, Jae Min
Choi, Haeyoun
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  givenname: Ah-Ra
  surname: Lee
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  surname: Choi
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  givenname: Sang-Hyun
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  givenname: Hee Il
  surname: Lee
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  organization: Division of Vectors and Parasitic Diseases, Korea Disease Control and Prevention Agency, Cheongju, Republic of Korea
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  givenname: Jae Min
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  organization: School of Biopharmaceutical and Medical Sciences, Sungshin Women's University, Seoul, Republic of Korea
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  givenname: Seong-Jun
  orcidid: 0000-0001-8504-2872
  surname: Kim
  fullname: Kim, Seong-Jun
  organization: Center for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon, Republic of Korea
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  givenname: Tomohiro
  orcidid: 0009-0005-7444-2738
  surname: Ishikawa
  fullname: Ishikawa, Tomohiro
  organization: Department of Microbiology, Dokkyo Medical University School of Medicine, Tochigi, Japan
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  givenname: Ji-Man
  orcidid: 0000-0002-0678-4964
  surname: Kang
  fullname: Kang, Ji-Man
  organization: Department of Pediatrics, Severance Children's Hospital, Yonsei University College of Medicine, Seoul, Republic of Korea
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  givenname: Hyeon-Seok
  surname: Eom
  fullname: Eom, Hyeon-Seok
  organization: Department of Hematology-Oncology, Center for Hematologic Malignancy, National Cancer Center, Goyang, Republic of Korea
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  surname: Seo
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  organization: Department of Microbiology, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea
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Keywords Japanese encephalitis virus
neutralizing antibody
vaccine
cross-reactivity
genotype V
Language English
License The Author(s) 2024. Published by Oxford University Press on behalf of Infectious Diseases Society of America. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact journals.permissions@oup.com.
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Snippet Japanese encephalitis (JE) has been predominantly controlled through vaccination. However, the isolation of JE virus (JEV) genotype V (GV) in China in 2009,...
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StartPage 1281
SubjectTerms Adolescent
Adult
Antibodies, Neutralizing - blood
Antibodies, Viral - blood
Child
Child, Preschool
Encephalitis Virus, Japanese - classification
Encephalitis Virus, Japanese - genetics
Encephalitis Virus, Japanese - immunology
Encephalitis, Japanese - immunology
Encephalitis, Japanese - prevention & control
Encephalitis, Japanese - virology
Female
Genotype
Humans
Infant
Japanese Encephalitis Vaccines - administration & dosage
Japanese Encephalitis Vaccines - immunology
Male
Neutralization Tests
Republic of Korea - epidemiology
Vaccines, Attenuated - immunology
Vaccines, Inactivated - immunology
Young Adult
Title Genotype III-Based Japanese Encephalitis Vaccines Exhibit Diminished Neutralizing Response to Reemerging Genotype V
URI https://www.ncbi.nlm.nih.gov/pubmed/39574290
Volume 231
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