Mitogenic, chemotactic, and synthetic responses of rat periodontal ligament fibroblastic cells to polypeptide growth factors in vitro

The mitogenic, chemotactic, and synthetic responses of rat periodontal ligament (PDL) fibroblastic cells to epidermal growth factor (EGF), transforming growth factor-beta (TGF-beta), recombinant human platelet-derived growth factor (rhPDGF)-AB, rhPDGF-BB, natural (n) PDGF-AB, and insulin-like growth...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Journal of periodontology (1970) Jg. 63; H. 6; S. 515
Hauptverfasser:	Matsuda, N, Lin, W L, Kumar, N M, Cho, M I, Genco, R J
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	United States 01.06.1992
Schlagworte:	Animals Cells, Cultured - cytology Cells, Cultured - drug effects Cells, Cultured - metabolism Chemotaxis - drug effects Collagen - biosynthesis Collagen - drug effects Female Fibroblasts - cytology Fibroblasts - drug effects Fibroblasts - metabolism Growth Substances - pharmacology Mitosis - drug effects Peptides - pharmacology Periodontal Ligament - cytology Periodontal Ligament - drug effects Periodontal Ligament - metabolism Rats Rats, Inbred Strains
ISSN:	0022-3492
Online-Zugang:	Weitere Angaben
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Abstract	The mitogenic, chemotactic, and synthetic responses of rat periodontal ligament (PDL) fibroblastic cells to epidermal growth factor (EGF), transforming growth factor-beta (TGF-beta), recombinant human platelet-derived growth factor (rhPDGF)-AB, rhPDGF-BB, natural (n) PDGF-AB, and insulin-like growth factor-I (IGF-I) were examined in vitro using PDL cells obtained from the coagulum of healing tooth sockets. PDGFs and IGF-I have potent and comparable mitogenic effects on PDL fibroblastic cells. The maximum mitogenic effect of PDGFs was observed at the concentration of 10 ng/ml, whereas that of IGF-I was seen at concentrations higher than 100 ng/ml. In contrast, EGF induced moderate, and TGF-beta inhibitory mitogenic responses. The combination of rhPDGF-AB with either EGF or TGF-beta demonstrated comparable mitogenic potency, equivalent to the level of PDGF alone regardless of the mitogenic effect of other growth factors. The combination of rhPDGF-AB and IGF-I, however, showed a synergistic effect revealing the highest mitogenic effect among all individual growth factors as well as any combinations of the growth factors tested. Similarly, PDL fibroblastic cells demonstrated strong chemotactic responses to both IGF-I and PDGFs. The maximum effect was observed by IGF-I at concentrations higher than 10 ng/ml, followed by rhPDGF-BB at 0.1 ng/ml, rhPDGF-AB and nPDGF at concentrations ranging from 0.1 to 1 ng/ml. TGF-beta revealed no, and EGF slightly increased, chemotactic effects. IGF-I slightly enhanced the synthesis of total protein, whereas other factors had no significant effect. However, both rhPDGF-AB and TGF-beta stimulated collagen synthesis. On the other hand, IGF-I showed no effect on collagen synthesis, while EGF suppressed collagen synthesis. These findings suggest that rhPDGF-BB and IGF-I stimulate proliferation and chemotaxis of PDL fibroblastic cells. In addition, the combination of these growth factors further increases the mitogenic effect. rhPDGF-AB also stimulates collagen synthesis by PDL fibroblastic cells. Thus, rhPDGF-BB and IGF-I may have important roles in promotion of PDL healing, and consequently, may be useful for clinical application in periodontal regenerative procedures.
AbstractList	The mitogenic, chemotactic, and synthetic responses of rat periodontal ligament (PDL) fibroblastic cells to epidermal growth factor (EGF), transforming growth factor-beta (TGF-beta), recombinant human platelet-derived growth factor (rhPDGF)-AB, rhPDGF-BB, natural (n) PDGF-AB, and insulin-like growth factor-I (IGF-I) were examined in vitro using PDL cells obtained from the coagulum of healing tooth sockets. PDGFs and IGF-I have potent and comparable mitogenic effects on PDL fibroblastic cells. The maximum mitogenic effect of PDGFs was observed at the concentration of 10 ng/ml, whereas that of IGF-I was seen at concentrations higher than 100 ng/ml. In contrast, EGF induced moderate, and TGF-beta inhibitory mitogenic responses. The combination of rhPDGF-AB with either EGF or TGF-beta demonstrated comparable mitogenic potency, equivalent to the level of PDGF alone regardless of the mitogenic effect of other growth factors. The combination of rhPDGF-AB and IGF-I, however, showed a synergistic effect revealing the highest mitogenic effect among all individual growth factors as well as any combinations of the growth factors tested. Similarly, PDL fibroblastic cells demonstrated strong chemotactic responses to both IGF-I and PDGFs. The maximum effect was observed by IGF-I at concentrations higher than 10 ng/ml, followed by rhPDGF-BB at 0.1 ng/ml, rhPDGF-AB and nPDGF at concentrations ranging from 0.1 to 1 ng/ml. TGF-beta revealed no, and EGF slightly increased, chemotactic effects. IGF-I slightly enhanced the synthesis of total protein, whereas other factors had no significant effect. However, both rhPDGF-AB and TGF-beta stimulated collagen synthesis. On the other hand, IGF-I showed no effect on collagen synthesis, while EGF suppressed collagen synthesis. These findings suggest that rhPDGF-BB and IGF-I stimulate proliferation and chemotaxis of PDL fibroblastic cells. In addition, the combination of these growth factors further increases the mitogenic effect. rhPDGF-AB also stimulates collagen synthesis by PDL fibroblastic cells. Thus, rhPDGF-BB and IGF-I may have important roles in promotion of PDL healing, and consequently, may be useful for clinical application in periodontal regenerative procedures.The mitogenic, chemotactic, and synthetic responses of rat periodontal ligament (PDL) fibroblastic cells to epidermal growth factor (EGF), transforming growth factor-beta (TGF-beta), recombinant human platelet-derived growth factor (rhPDGF)-AB, rhPDGF-BB, natural (n) PDGF-AB, and insulin-like growth factor-I (IGF-I) were examined in vitro using PDL cells obtained from the coagulum of healing tooth sockets. PDGFs and IGF-I have potent and comparable mitogenic effects on PDL fibroblastic cells. The maximum mitogenic effect of PDGFs was observed at the concentration of 10 ng/ml, whereas that of IGF-I was seen at concentrations higher than 100 ng/ml. In contrast, EGF induced moderate, and TGF-beta inhibitory mitogenic responses. The combination of rhPDGF-AB with either EGF or TGF-beta demonstrated comparable mitogenic potency, equivalent to the level of PDGF alone regardless of the mitogenic effect of other growth factors. The combination of rhPDGF-AB and IGF-I, however, showed a synergistic effect revealing the highest mitogenic effect among all individual growth factors as well as any combinations of the growth factors tested. Similarly, PDL fibroblastic cells demonstrated strong chemotactic responses to both IGF-I and PDGFs. The maximum effect was observed by IGF-I at concentrations higher than 10 ng/ml, followed by rhPDGF-BB at 0.1 ng/ml, rhPDGF-AB and nPDGF at concentrations ranging from 0.1 to 1 ng/ml. TGF-beta revealed no, and EGF slightly increased, chemotactic effects. IGF-I slightly enhanced the synthesis of total protein, whereas other factors had no significant effect. However, both rhPDGF-AB and TGF-beta stimulated collagen synthesis. On the other hand, IGF-I showed no effect on collagen synthesis, while EGF suppressed collagen synthesis. These findings suggest that rhPDGF-BB and IGF-I stimulate proliferation and chemotaxis of PDL fibroblastic cells. In addition, the combination of these growth factors further increases the mitogenic effect. rhPDGF-AB also stimulates collagen synthesis by PDL fibroblastic cells. Thus, rhPDGF-BB and IGF-I may have important roles in promotion of PDL healing, and consequently, may be useful for clinical application in periodontal regenerative procedures. The mitogenic, chemotactic, and synthetic responses of rat periodontal ligament (PDL) fibroblastic cells to epidermal growth factor (EGF), transforming growth factor-beta (TGF-beta), recombinant human platelet-derived growth factor (rhPDGF)-AB, rhPDGF-BB, natural (n) PDGF-AB, and insulin-like growth factor-I (IGF-I) were examined in vitro using PDL cells obtained from the coagulum of healing tooth sockets. PDGFs and IGF-I have potent and comparable mitogenic effects on PDL fibroblastic cells. The maximum mitogenic effect of PDGFs was observed at the concentration of 10 ng/ml, whereas that of IGF-I was seen at concentrations higher than 100 ng/ml. In contrast, EGF induced moderate, and TGF-beta inhibitory mitogenic responses. The combination of rhPDGF-AB with either EGF or TGF-beta demonstrated comparable mitogenic potency, equivalent to the level of PDGF alone regardless of the mitogenic effect of other growth factors. The combination of rhPDGF-AB and IGF-I, however, showed a synergistic effect revealing the highest mitogenic effect among all individual growth factors as well as any combinations of the growth factors tested. Similarly, PDL fibroblastic cells demonstrated strong chemotactic responses to both IGF-I and PDGFs. The maximum effect was observed by IGF-I at concentrations higher than 10 ng/ml, followed by rhPDGF-BB at 0.1 ng/ml, rhPDGF-AB and nPDGF at concentrations ranging from 0.1 to 1 ng/ml. TGF-beta revealed no, and EGF slightly increased, chemotactic effects. IGF-I slightly enhanced the synthesis of total protein, whereas other factors had no significant effect. However, both rhPDGF-AB and TGF-beta stimulated collagen synthesis. On the other hand, IGF-I showed no effect on collagen synthesis, while EGF suppressed collagen synthesis. These findings suggest that rhPDGF-BB and IGF-I stimulate proliferation and chemotaxis of PDL fibroblastic cells. In addition, the combination of these growth factors further increases the mitogenic effect. rhPDGF-AB also stimulates collagen synthesis by PDL fibroblastic cells. Thus, rhPDGF-BB and IGF-I may have important roles in promotion of PDL healing, and consequently, may be useful for clinical application in periodontal regenerative procedures.
Author	Kumar, N M Genco, R J Cho, M I Matsuda, N Lin, W L
Author_xml	– sequence: 1 givenname: N surname: Matsuda fullname: Matsuda, N organization: Department of Oral Biology, School of Dental Medicine, State University of New York, Buffalo – sequence: 2 givenname: W L surname: Lin fullname: Lin, W L – sequence: 3 givenname: N M surname: Kumar fullname: Kumar, N M – sequence: 4 givenname: M I surname: Cho fullname: Cho, M I – sequence: 5 givenname: R J surname: Genco fullname: Genco, R J
BackLink	https://www.ncbi.nlm.nih.gov/pubmed/1625151$$D View this record in MEDLINE/PubMed
BookMark	eNotkL1OwzAQgD0UlVJ4AAYkT0wk2GcnbUZU8ScVscAc2cmldZXYxnZBfQDem1R0uvtO3_3oLsjEOouEXHOW84rB_c75MakgL0Ve5gUvJmTGGEAmZAXn5CLG3YhcCjYlU17CaPAZ-X0zyW3QmuaONlscXFJNOoKyLY0Hm7Y4Ig0YvbMRI3UdDSpRj8G41tmketqbjRrQJtoZHZzuVTy2NNj3kSZHvesPHn0yLdJNcD9pS7txiQuRGku_TQrukpx1qo94dYpz8vn0-LF6ydbvz6-rh3XWCCiKTKOWHEFiy9qyA91I4ACLTugF05XsSl51xVgVDJBBK5ZKSaVFWVUFLKSoYE5u_-f64L72GFM9mHg8VFl0-1gvBCuEXIpRvDmJez1gW_tgBhUO9elv8AfrK3K6
CitedBy_id	crossref_primary_10_1111_j_1708_8208_2010_00327_x crossref_primary_10_1016_j_bbrc_2006_03_106 crossref_primary_10_1046_j_1365_2591_1999_00207_x crossref_primary_10_1016_S0142_9612_99_00143_X crossref_primary_10_1038_s41467_017_01955_w crossref_primary_10_1016_S0011_8532_22_00594_8 crossref_primary_10_1111_j_1600_0765_2004_00750_x crossref_primary_10_1902_jop_2008_070327 crossref_primary_10_1177_03635465990270051601 crossref_primary_10_1007_BF00298592 crossref_primary_10_1016_S0003_9969_01_00036_X crossref_primary_10_2319_0003_3219_2008_078_0610_OSIOTE_2_0_CO_2 crossref_primary_10_1002_cm_20123 crossref_primary_10_1111_j_1600_051X_1996_tb00496_x crossref_primary_10_1111_j_1600_0765_1996_tb00497_x crossref_primary_10_1111_j_1601_5037_2009_00380_x crossref_primary_10_1111_j_1600_0765_1998_tb02205_x crossref_primary_10_1007_BF00298997 crossref_primary_10_1002_jcb_20605 crossref_primary_10_1006_excr_2001_5208 crossref_primary_10_1111_j_1600_0765_1999_tb02250_x crossref_primary_10_1016_j_joms_2007_06_635 crossref_primary_10_1111_j_1600_0765_2007_01062_x crossref_primary_10_1006_bbrc_1998_9151 crossref_primary_10_1007_BF03179936 crossref_primary_10_1016_j_cden_2009_09_001 crossref_primary_10_1089_ten_tea_2016_0163 crossref_primary_10_1902_jop_2012_120141 crossref_primary_10_1902_jop_2006_050147 crossref_primary_10_1016_j_addr_2007_03_016 crossref_primary_10_1177_00220345940730100301 crossref_primary_10_1111_etp_12020 crossref_primary_10_1111_j_1600_0765_2010_01313_x crossref_primary_10_1007_s00784_019_03088_4 crossref_primary_10_1016_S0168_3659_01_00498_9 crossref_primary_10_1111_j_1600_051X_2007_01060_x crossref_primary_10_1111_j_1600_051X_2005_00870_x crossref_primary_10_1111_j_1600_9657_2011_01070_x crossref_primary_10_1111_j_1600_0765_1996_tb00485_x crossref_primary_10_1111_j_1600_0765_2008_01125_x crossref_primary_10_1016_j_cden_2005_12_001 crossref_primary_10_1111_j_1600_0765_1996_tb00481_x crossref_primary_10_1111_jre_13244 crossref_primary_10_1631_jzus_B1300062 crossref_primary_10_1111_j_1600_051X_1994_tb00733_x crossref_primary_10_1111_j_1600_051x_2004_00521_x crossref_primary_10_1177_0885328216686727 crossref_primary_10_1177_00220345960750090801 crossref_primary_10_1111_j_1600_051X_2005_00663_x crossref_primary_10_1111_j_1600_0765_1997_tb01380_x crossref_primary_10_1902_jop_2014_140083 crossref_primary_10_1111_jre_12557 crossref_primary_10_1016_j_archoralbio_2006_04_008 crossref_primary_10_3892_etm_2015_2414 crossref_primary_10_1016_S0099_2399_97_80399_8 crossref_primary_10_1111_j_1600_051X_2010_01639_x crossref_primary_10_1034_j_1600_0765_2003_01607_x crossref_primary_10_1111_j_1600_051X_1998_tb02363_x crossref_primary_10_1007_BF02522484 crossref_primary_10_1177_1534734614534979 crossref_primary_10_1002_jcp_1041620308 crossref_primary_10_1002_cap_10211 crossref_primary_10_1177_00220345010800071301 crossref_primary_10_1902_jop_2007_060302 crossref_primary_10_1016_j_jconrel_2010_10_021 crossref_primary_10_1111_prd_12062 crossref_primary_10_1111_j_1600_0765_1999_tb02277_x crossref_primary_10_1177_00220345970760090901 crossref_primary_10_1067_mod_2000_104097 crossref_primary_10_1089_ten_2006_12_ft_93 crossref_primary_10_1007_s10787_998_0021_5 crossref_primary_10_1016_j_jmbbm_2018_12_012 crossref_primary_10_1111_j_1600_0757_1993_tb00233_x crossref_primary_10_35607_kjoa_34_1_201311_002 crossref_primary_10_1016_j_drudis_2010_05_003 crossref_primary_10_1016_j_jconrel_2011_07_004 crossref_primary_10_1002_jbm_b_30337 crossref_primary_10_3390_biom12030435 crossref_primary_10_1016_j_archoralbio_2017_09_019 crossref_primary_10_1053_j_oto_2009_10_006 crossref_primary_10_1080_08977190601060990 crossref_primary_10_1089_ten_tea_2018_0335 crossref_primary_10_1111_j_1600_0501_2011_02345_x crossref_primary_10_4028_www_scientific_net_KEM_441_181 crossref_primary_10_3109_03008200009005642 crossref_primary_10_1002_jcb_29706 crossref_primary_10_1111_j_1600_0757_2006_00162_x crossref_primary_10_1016_j_bbrc_2005_08_182 crossref_primary_10_4137_BTRI_S8120 crossref_primary_10_1007_s10103_007_0477_3 crossref_primary_10_1111_j_1834_7819_1997_tb06081_x crossref_primary_10_1111_j_1601_0825_1995_tb00191_x crossref_primary_10_1177_10454411970080030201 crossref_primary_10_1016_S0718_5391_12_70110_6 crossref_primary_10_1111_j_1472_8206_2009_00786_x crossref_primary_10_1111_jcpe_12030 crossref_primary_10_1111_j_1600_051X_1995_tb00826_x crossref_primary_10_3390_jpm11100944 crossref_primary_10_1016_j_cyto_2007_06_009 crossref_primary_10_1177_00220345960750030601 crossref_primary_10_1111_j_1600_0722_1997_tb00210_x crossref_primary_10_1016_0003_9969_93_90120_B crossref_primary_10_1111_jre_12935 crossref_primary_10_1111_prd_12522 crossref_primary_10_1016_S0168_3659_00_00232_7 crossref_primary_10_1902_jop_2007_060442 crossref_primary_10_1067_mse_2001_109559 crossref_primary_10_1002__SICI_1097_0185_199606_245_2_342__AID_AR16_3_0_CO_2_P crossref_primary_10_1080_15321810903084673 crossref_primary_10_1007_s10266_011_0046_x crossref_primary_10_1111_j_1600_0765_1999_tb02278_x crossref_primary_10_1517_17425247_3_5_647 crossref_primary_10_1039_c5ib00114e crossref_primary_10_1177_159101999800400102 crossref_primary_10_1034_j_1600_0765_2003_00640_x crossref_primary_10_1111_jre_12921 crossref_primary_10_1002__SICI_1097_4652_199803_174_3_353__AID_JCP9_3_0_CO_2_N crossref_primary_10_52711_0974_360X_2024_00106 crossref_primary_10_1089_pho_2011_3079 crossref_primary_10_1089_107632703768247421 crossref_primary_10_1902_jop_2007_070119 crossref_primary_10_1177_00220345990780100701 crossref_primary_10_1902_jop_2009_080502 crossref_primary_10_1128_IAI_00830_12 crossref_primary_10_1097_ID_0b013e3181dc24a9 crossref_primary_10_1016_S0003_9969_00_00099_6 crossref_primary_10_1177_00220345990780060901 crossref_primary_10_1016_0003_9969_94_90150_3 crossref_primary_10_3390_ijms23126552 crossref_primary_10_3390_ijms24098239 crossref_primary_10_1089_pho_2010_2846 crossref_primary_10_1016_j_ymthe_2004_01_016 crossref_primary_10_1111_j_1600_0765_1999_tb02245_x crossref_primary_10_1002_jor_1100170315 crossref_primary_10_1111_j_1600_051X_1998_tb02500_x crossref_primary_10_1007_s10439_014_1125_2 crossref_primary_10_1097_00004770_200203000_00012 crossref_primary_10_1111_j_1600_0765_1998_tb02336_x crossref_primary_10_1902_jop_2011_110144 crossref_primary_10_1016_j_jconrel_2006_05_014 crossref_primary_10_3109_03008200109016843 crossref_primary_10_1111_j_1600_051X_1998_tb02463_x crossref_primary_10_1016_j_cellbi_2003_12_007 crossref_primary_10_1016_S0003_9969_96_00024_6 crossref_primary_10_1111_j_1600_051X_2007_01090_x crossref_primary_10_1016_S1532_3382_12_70025_8 crossref_primary_10_1111_j_1600_0757_2011_00432_x crossref_primary_10_1016_j_archoralbio_2005_10_005 crossref_primary_10_1111_j_1600_0765_1997_tb00577_x crossref_primary_10_1111_j_1601_0825_2004_00992_x crossref_primary_10_1111_j_1600_0765_2009_01268_x crossref_primary_10_1111_j_1524_475X_2008_00376_x crossref_primary_10_1111_j_1600_0765_1999_tb02275_x crossref_primary_10_1177_00220345960750041401 crossref_primary_10_1034_j_1600_9657_2001_170106_x crossref_primary_10_1111_j_1600_0765_1998_tb02325_x crossref_primary_10_1016_S0168_3659_97_00169_7 crossref_primary_10_1111_j_1600_0765_2008_01089_x crossref_primary_10_1002_ar_1092400407 crossref_primary_10_1038_sj_bdj_2011_523 crossref_primary_10_1016_S8756_3282_96_00127_5 crossref_primary_10_1089_ten_2006_12_1441 crossref_primary_10_1177_00220345980770100601 crossref_primary_10_1089_ten_teb_2008_0062 crossref_primary_10_1902_jop_2008_070436 crossref_primary_10_1053_otsm_2002_31604 crossref_primary_10_1002_jor_1100130206 crossref_primary_10_1002__SICI_1097_0185_199606_245_2_361__AID_AR17_3_0_CO_2_N crossref_primary_10_1002_JPER_19_0352 crossref_primary_10_1111_j_1600_0722_2007_00469_x crossref_primary_10_1016_S0003_9969_99_00020_5 crossref_primary_10_1016_j_joen_2005_11_007 crossref_primary_10_1111_j_1600_051X_1997_tb00761_x crossref_primary_10_1016_S0014_5793_98_00695_4 crossref_primary_10_1177_10454411980090030101 crossref_primary_10_1016_j_reactfunctpolym_2014_04_003 crossref_primary_10_1111_j_1600_0765_1997_tb01400_x crossref_primary_10_1034_j_1600_0722_2002_21340_x crossref_primary_10_1152_japplphysiol_01150_2014 crossref_primary_10_1097_BTF_0b013e3182463ca1 crossref_primary_10_1111_j_1600_0757_1997_tb00094_x crossref_primary_10_1034_j_1601_0825_2003_02906_x
ContentType	Journal Article
DBID	CGR CUY CVF ECM EIF NPM 7X8
DOI	10.1902/jop.1992.63.6.515
DatabaseName	Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed MEDLINE - Academic
DatabaseTitle	MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) MEDLINE - Academic
DatabaseTitleList	MEDLINE - Academic MEDLINE
Database_xml	– sequence: 1 dbid: NPM name: PubMed url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: 7X8 name: MEDLINE - Academic url: https://search.proquest.com/medline sourceTypes: Aggregation Database
DeliveryMethod	no_fulltext_linktorsrc
Discipline	Dentistry
ExternalDocumentID	1625151
Genre	Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S Journal Article
GrantInformation_xml	– fundername: NIDCR NIH HHS grantid: DE 08240
GroupedDBID	--- -Y1 .55 0R~ 1OB 1OC 33P 34H 53G 5GY 5RE AAEUS AAHQN AAIPD AAMMB AAMNL AANLZ AAQQT AASGY AAWTL AAXRX AAYCA AAZKR ABCUV ABDQB ABJNI ABLJU ABOCM ABQWH ABXGK ACAHQ ACCZN ACGFO ACGFS ACGOF ACMXC ACPOU ACXBN ACXQS ADBBV ADBTR ADEOM ADKYN ADMGS ADOZA ADXAS ADZMN AEFGJ AEIGN AENEX AEUYR AEYWJ AFFNX AFFPM AFGKR AFWVQ AGHNM AGXDD AGYGG AHBTC AIACR AIDQK AIDYY AITYG AIURR ALMA_UNASSIGNED_HOLDINGS ALUQN ALVPJ AMYDB BFHJK C45 CGR CS3 CUY CVF CWXXS DCZOG DRFUL DRMAN DRSTM DU5 EBS ECM EIF EJD F5P FUBAC GK1 HGLYW KBYEO L7B LATKE LEEKS LUTES LYRES MEWTI MSFUL MSMAN MSSTM MXFUL MXMAN MXSTM NPM O9- OVD P2P P2W PER RJQFR ROL SAMSI SJN SUPJJ TEORI UCV UMD USG W99 WH7 WOHZO WXSBR X7M XZL YOJ ZE2 ZGI ZVN ZZTAW 7X8 AIQQE
ID	FETCH-LOGICAL-c3255-beb41e24ed0d6f2bc421227f3b70b94f619f5bc4302e02d38aa4ab36995274392
IEDL.DBID	7X8
ISSN	0022-3492
IngestDate	Sat Sep 27 22:05:18 EDT 2025 Tue Aug 05 11:39:48 EDT 2025
IsPeerReviewed	true
IsScholarly	true
Issue	6
Language	English
LinkModel	DirectLink
MergedId	FETCHMERGED-LOGICAL-c3255-beb41e24ed0d6f2bc421227f3b70b94f619f5bc4302e02d38aa4ab36995274392
Notes	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
PMID	1625151
PQID	73053483
PQPubID	23479
ParticipantIDs	proquest_miscellaneous_73053483 pubmed_primary_1625151
PublicationCentury	1900
PublicationDate	June 1992
PublicationDateYYYYMMDD	1992-06-01
PublicationDate_xml	– month: 06 year: 1992 text: June 1992
PublicationDecade	1990
PublicationPlace	United States
PublicationPlace_xml	– name: United States
PublicationTitle	Journal of periodontology (1970)
PublicationTitleAlternate	J Periodontol
PublicationYear	1992
SSID	ssj0021430
Score	1.8323259
Snippet	The mitogenic, chemotactic, and synthetic responses of rat periodontal ligament (PDL) fibroblastic cells to epidermal growth factor (EGF), transforming growth...
SourceID	proquest pubmed
SourceType	Aggregation Database Index Database
StartPage	515
SubjectTerms	Animals Cells, Cultured - cytology Cells, Cultured - drug effects Cells, Cultured - metabolism Chemotaxis - drug effects Collagen - biosynthesis Collagen - drug effects Female Fibroblasts - cytology Fibroblasts - drug effects Fibroblasts - metabolism Growth Substances - pharmacology Mitosis - drug effects Peptides - pharmacology Periodontal Ligament - cytology Periodontal Ligament - drug effects Periodontal Ligament - metabolism Rats Rats, Inbred Strains
Title	Mitogenic, chemotactic, and synthetic responses of rat periodontal ligament fibroblastic cells to polypeptide growth factors in vitro
URI	https://www.ncbi.nlm.nih.gov/pubmed/1625151 https://www.proquest.com/docview/73053483
Volume	63
hasFullText
inHoldings	1
isFullTextHit
isPrint
link	http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1LS8QwEA7rA_TiW3w7B4927ebRpiCIqIsXFw8Ke1uSNtEVadbdVfAH-L-dabvexIOXQgqBkPkymS_zYuwkFVTFLE0ir1MXSR2rSGeJjzq5p3Jc1hljqmYTaa-n-_3svsXOZ7kwFFY504mVoi5CTm_kZ4hEJaQWF6O3iHpGkW-1aaAxxxYEGjJ0LNP-jw-BoyUQ_9QKlxlvfJpZzM9ewojS9Hg7Ee2krTrqd_uyume6q_9b4RpbaexLuKwBsc5artxgS9cUE0Rt3TbZ1x2eYUTNMD8FFBhKqkqTOgVTFjD5LNEgxCGM69hZN4HgAVECVBEZKSwlT8Lr8MnQqyJ4JNvBogFOU8gHMIFpgFF4RXaLuqhw8IQ0f_oMTVsfGJbwMZyOwxZ77N48XN1GTS-GKBfIOiLrrOw4Ll0RF4nnNidPMk-9sGlsM-mRh3mFf0XMXcwLoY2RxookyxQnzsO32XwZSrfDINPea8VRc3gpOV6H2iIoEqmUtgKxs8uOZ_s7QKzT4k3pwvtkMNvhXbZdi2gwqktyDDpI49B22ftz6j5brgNu6R3lgC14POTukC3mHyiG8VGFIPz27u--AVZt0hM
linkProvider	ProQuest
openUrl	ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Mitogenic%2C+chemotactic%2C+and+synthetic+responses+of+rat+periodontal+ligament+fibroblastic+cells+to+polypeptide+growth+factors+in+vitro&rft.jtitle=Journal+of+periodontology+%281970%29&rft.au=Matsuda%2C+N&rft.au=Lin%2C+W+L&rft.au=Kumar%2C+N+M&rft.au=Cho%2C+M+I&rft.date=1992-06-01&rft.issn=0022-3492&rft.volume=63&rft.issue=6&rft.spage=515&rft_id=info:doi/10.1902%2Fjop.1992.63.6.515&rft.externalDBID=NO_FULL_TEXT
thumbnail_l	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0022-3492&client=summon
thumbnail_m	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0022-3492&client=summon
thumbnail_s	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0022-3492&client=summon