Proteome Analysis Using Gel-LC-MS/MS

This article describes processing of protein samples using 1D SDS gels prior to protease digestion for proteomics workflows that subsequently utilize reversed-phase nanocapillary ultra-high-pressure liquid chromatography (LC) coupled to tandem mass spectrometry (MS/MS). The resulting LC-MS/MS data a...

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Veröffentlicht in:	Current protocols in protein science Jg. 96; H. 1; S. e93
Hauptverfasser:	Goldman, Aaron R, Beer, Lynn A, Tang, Hsin-Yao, Hembach, Peter, Zayas-Bazan, Delaine, Speicher, David W
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	United States 01.06.2019
Schlagworte:	Chemical Fractionation Chromatography, High Pressure Liquid - methods Electrophoresis, Polyacrylamide Gel High-Throughput Screening Assays - instrumentation High-Throughput Screening Assays - methods Peptides - chemistry Proteome - analysis Tandem Mass Spectrometry proteome fractionation LC-MS/MS proteomics in-gel digestion gel-LC-MS/MS mass spectrometry SDS gels
ISSN:	1934-3663, 1934-3663
Online-Zugang:	Weitere Angaben
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