Magnetic microsphere based droplet microarray for high-throughput screening of multiple chemical residues in milk

In this paper, aflatoxin M1, sulfamethoxazole, ciprofloxacin, and lincomycin were selected as model analytes, and we introduced a novel quadplex droplet microarray format for simultaneous screening of multiple chemical residues in milk samples. The droplet microarray relied on micro-volume shallow-w...

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Veröffentlicht in:Food chemistry Jg. 492; H. Pt 2; S. 145509
Hauptverfasser: Liu, Chen, Zhai, Peng, Luo, Pengjie, Zhang, Huiya, Zou, Boyu, Liao, Meiting, Jiang, Wenxiao
Format: Journal Article
Sprache:Englisch
Veröffentlicht: England Elsevier Ltd 15.11.2025
Schlagworte:
Aflatoxin M1 - analysis
Animals
Antibacterial agents
Cattle
Ciprofloxacin - analysis
Droplet microarray
Drug Residues - analysis
Food Contamination - analysis
Food safety
High-Throughput Screening Assays - instrumentation
High-Throughput Screening Assays - methods
Limit of Detection
Microarray Analysis - instrumentation
Microarray Analysis - methods
Microspheres
Milk - chemistry
Mycotoxins
Quadplexed immunoassay
Sulfamethoxazole - analysis
Food safety
Quadplexed immunoassay
Droplet microarray
Antibacterial agents
Mycotoxins
ISSN:0308-8146, 1873-7072, 1873-7072
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Zusammenfassung:In this paper, aflatoxin M1, sulfamethoxazole, ciprofloxacin, and lincomycin were selected as model analytes, and we introduced a novel quadplex droplet microarray format for simultaneous screening of multiple chemical residues in milk samples. The droplet microarray relied on micro-volume shallow-well microplate combined with microspheres-based suspension array. In the new established droplet microarrays, the consumption of samples and immunoreagents was reduced by 80 %. While, the antigen-antibody binding process can be completed in as little as 5 min. The limit of detection was evaluated to be 0.023 μg/kg for aflatoxin M1, 5.68 μg/kg for sulfamethoxazole, 0.85 μg/kg for ciprofloxacin, and 2.65 μg/kg for lincomycin. The recoveries of the target analytes from spiked milk were 79.7 %–102.6 %, with the coefficients of variation not larger than 10.6 %. The newly developed droplet microarray has been demonstrated with good validity and sensitivity by comparing it with the conventional immunoassays and instrumental methods. •A micro-volume shallow-well microplate was fabricated for new immunoassay development.•A quadplex droplet microarray was developed for screening of multiple chemical residues in milk.•The sample usage and the consumption of immuno-reagents could be reduced by 80 %.•The antigen-antibody binding process can be completed within 5 min.•The developed assay format could be expanded to other chemical analysis.
Bibliographie:ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 23
ISSN:0308-8146
1873-7072
1873-7072
DOI:10.1016/j.foodchem.2025.145509