Dopamine disruption and autism phenotypes in slc6a3−/− zebrafish: Behavioural and molecular insights

Dopamine plays a crucial role in regulating movement, motivation, attention, and emotions. Disruptions in dopamine metabolism have been linked to various psychiatric disorders, including autism spectrum disorder (ASD). In this study, we generated an slc6a3 knockout zebrafish model using the CRISPR-C...

Celý popis

Uloženo v:

Podrobná bibliografie
Vydáno v:	Progress in neuro-psychopharmacology & biological psychiatry Ročník 143; s. 111528
Hlavní autoři:	Li, Wen, Zhang, Xiaocong, Niu, Xiaoyu, Qin, Nan, Kang, Lulu, Wang, Kai, Wang, Mingyong
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	England Elsevier Inc 20.12.2025
Témata:	Autism spectrum disorder Behavioural analysis Dopamine dysfunction slc6a3 Zebrafish model AB mpf slc6a3 Behavioural analysis ARRIVE KO Zebrafish model HPLC-ECD IHC Autism spectrum disorder SD RNA-seq TH ANOVA Dopamine dysfunction IACUC qPCR SEM dpf DA ELISA ISH
ISSN:	0278-5846, 1878-4216, 1878-4216
On-line přístup:	Získat plný text
Tagy:	Přidat tag Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!

Abstract	Dopamine plays a crucial role in regulating movement, motivation, attention, and emotions. Disruptions in dopamine metabolism have been linked to various psychiatric disorders, including autism spectrum disorder (ASD). In this study, we generated an slc6a3 knockout zebrafish model using the CRISPR-Cas9 system to investigate the relationship between dopamine dysfunction and autism. Our results revealed that slc6a3 knockout significantly reduced dopamine levels, leading to impaired dopamine synthesis, transport, and metabolism. Behavioural analysis demonstrated that slc6a3−/− zebrafish exhibited decreased motor activity, increased anxiety-like behaviour, and autism-related symptoms, such as impaired social ability and “digging” behaviour. Pharmacological intervention with risperidone and clozapine improved motor function, social interaction, and anxiety levels, with risperidone showing superior effects. Transcriptomic analysis identified significant changes in several nervous system-related genes in slc6a3−/− zebrafish, suggesting that these gene alterations may contribute to the observed behavioural abnormalities. Our study highlights the crucial role of dopamine dysfunction in autism and establishes slc6a3−/− zebrafish as a valuable model for studying autism and screening potential therapeutic drugs. •Generation of a CRISPR/Cas9-based zebrafish slc6a3 knockout model mimicking dopamine transporter deficiency.•Comprehensive behavioural phenotyping across developmental stages revealed locomotor, anxiety-like, and social interaction deficits.•Pharmacological rescue with risperidone and clozapine demonstrated predictive validity and differential efficacy in the zebrafish model.•Molecular and transcriptomic analyses identified dopaminergic pathway alterations and potential compensatory mechanisms.•Zebrafish slc6a3 mutants provide a translational platform for investigating dopamine-related neurodevelopmental disorders and drug screening.
AbstractList	Dopamine plays a crucial role in regulating movement, motivation, attention, and emotions. Disruptions in dopamine metabolism have been linked to various psychiatric disorders, including autism spectrum disorder (ASD). In this study, we generated an slc6a3 knockout zebrafish model using the CRISPR-Cas9 system to investigate the relationship between dopamine dysfunction and autism. Our results revealed that slc6a3 knockout significantly reduced dopamine levels, leading to impaired dopamine synthesis, transport, and metabolism. Behavioural analysis demonstrated that slc6a3-/- zebrafish exhibited decreased motor activity, increased anxiety-like behaviour, and autism-related symptoms, such as impaired social ability and "digging" behaviour. Pharmacological intervention with risperidone and clozapine improved motor function, social interaction, and anxiety levels, with risperidone showing superior effects. Transcriptomic analysis identified significant changes in several nervous system-related genes in slc6a3-/- zebrafish, suggesting that these gene alterations may contribute to the observed behavioural abnormalities. Our study highlights the crucial role of dopamine dysfunction in autism and establishes slc6a3-/- zebrafish as a valuable model for studying autism and screening potential therapeutic drugs. Dopamine plays a crucial role in regulating movement, motivation, attention, and emotions. Disruptions in dopamine metabolism have been linked to various psychiatric disorders, including autism spectrum disorder (ASD). In this study, we generated an slc6a3 knockout zebrafish model using the CRISPR-Cas9 system to investigate the relationship between dopamine dysfunction and autism. Our results revealed that slc6a3 knockout significantly reduced dopamine levels, leading to impaired dopamine synthesis, transport, and metabolism. Behavioural analysis demonstrated that slc6a3−/− zebrafish exhibited decreased motor activity, increased anxiety-like behaviour, and autism-related symptoms, such as impaired social ability and “digging” behaviour. Pharmacological intervention with risperidone and clozapine improved motor function, social interaction, and anxiety levels, with risperidone showing superior effects. Transcriptomic analysis identified significant changes in several nervous system-related genes in slc6a3−/− zebrafish, suggesting that these gene alterations may contribute to the observed behavioural abnormalities. Our study highlights the crucial role of dopamine dysfunction in autism and establishes slc6a3−/− zebrafish as a valuable model for studying autism and screening potential therapeutic drugs. •Generation of a CRISPR/Cas9-based zebrafish slc6a3 knockout model mimicking dopamine transporter deficiency.•Comprehensive behavioural phenotyping across developmental stages revealed locomotor, anxiety-like, and social interaction deficits.•Pharmacological rescue with risperidone and clozapine demonstrated predictive validity and differential efficacy in the zebrafish model.•Molecular and transcriptomic analyses identified dopaminergic pathway alterations and potential compensatory mechanisms.•Zebrafish slc6a3 mutants provide a translational platform for investigating dopamine-related neurodevelopmental disorders and drug screening. Dopamine plays a crucial role in regulating movement, motivation, attention, and emotions. Disruptions in dopamine metabolism have been linked to various psychiatric disorders, including autism spectrum disorder (ASD). In this study, we generated an slc6a3 knockout zebrafish model using the CRISPR-Cas9 system to investigate the relationship between dopamine dysfunction and autism. Our results revealed that slc6a3 knockout significantly reduced dopamine levels, leading to impaired dopamine synthesis, transport, and metabolism. Behavioural analysis demonstrated that slc6a3-/- zebrafish exhibited decreased motor activity, increased anxiety-like behaviour, and autism-related symptoms, such as impaired social ability and "digging" behaviour. Pharmacological intervention with risperidone and clozapine improved motor function, social interaction, and anxiety levels, with risperidone showing superior effects. Transcriptomic analysis identified significant changes in several nervous system-related genes in slc6a3-/- zebrafish, suggesting that these gene alterations may contribute to the observed behavioural abnormalities. Our study highlights the crucial role of dopamine dysfunction in autism and establishes slc6a3-/- zebrafish as a valuable model for studying autism and screening potential therapeutic drugs.Dopamine plays a crucial role in regulating movement, motivation, attention, and emotions. Disruptions in dopamine metabolism have been linked to various psychiatric disorders, including autism spectrum disorder (ASD). In this study, we generated an slc6a3 knockout zebrafish model using the CRISPR-Cas9 system to investigate the relationship between dopamine dysfunction and autism. Our results revealed that slc6a3 knockout significantly reduced dopamine levels, leading to impaired dopamine synthesis, transport, and metabolism. Behavioural analysis demonstrated that slc6a3-/- zebrafish exhibited decreased motor activity, increased anxiety-like behaviour, and autism-related symptoms, such as impaired social ability and "digging" behaviour. Pharmacological intervention with risperidone and clozapine improved motor function, social interaction, and anxiety levels, with risperidone showing superior effects. Transcriptomic analysis identified significant changes in several nervous system-related genes in slc6a3-/- zebrafish, suggesting that these gene alterations may contribute to the observed behavioural abnormalities. Our study highlights the crucial role of dopamine dysfunction in autism and establishes slc6a3-/- zebrafish as a valuable model for studying autism and screening potential therapeutic drugs.
ArticleNumber	111528
Author	Kang, Lulu Niu, Xiaoyu Wang, Mingyong Zhang, Xiaocong Wang, Kai Li, Wen Qin, Nan
Author_xml	– sequence: 1 givenname: Wen surname: Li fullname: Li, Wen organization: Department of Pediatrics, 1st Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, Henan Province, China – sequence: 2 givenname: Xiaocong surname: Zhang fullname: Zhang, Xiaocong organization: Department of Pediatrics, 1st Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, Henan Province, China – sequence: 3 givenname: Xiaoyu surname: Niu fullname: Niu, Xiaoyu organization: Department of Pediatrics, 1st Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, Henan Province, China – sequence: 4 givenname: Nan surname: Qin fullname: Qin, Nan organization: Department of Pediatrics, 1st Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, Henan Province, China – sequence: 5 givenname: Lulu surname: Kang fullname: Kang, Lulu organization: Department of Pediatrics, 1st Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, Henan Province, China – sequence: 6 givenname: Kai surname: Wang fullname: Wang, Kai email: wwwkkk8815@163.com organization: Department of Pediatrics, 1st Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, Henan Province, China – sequence: 7 givenname: Mingyong surname: Wang fullname: Wang, Mingyong email: maihe123456789@163.com organization: Murui Biological Technology Co., Ltd., Suzhou Industrial Park, Suzhou, China
BackLink	https://www.ncbi.nlm.nih.gov/pubmed/41101580$$D View this record in MEDLINE/PubMed
BookMark	eNp9kc1O3DAQx62Kqiy0T4BU5djLLv6IjYPEodAPKiH1wt2a2BPiVWK7doK0fYKe-4g8SQMLHHsYzRx-v5Fm_kfkIMSAhJwwumGUqdPtJoXUpg2nXG4YY5LrN2TF9Jle15ypA7KifJmlrtUhOSplSyllgop35LBmywap6Yr0X2KC0QesnC95TpOPoYLgKpgnX8Yq9RjitEtYKh-qMlgF4uHP39Olqt_YZuh86c-rS-zh3sc5w_Bkj3FAOw-QF6v4u34q78nbDoaCH577Mbn99vX26np98_P7j6vPN2srGJvWHdK2aZiTmksU4FrV1MA1UCWpbCl3wnLoRINWg3LoWA2dU6Jx8gypbMQx-bRfm3L8NWOZzOiLxWGAgHEuRnDFOde15gv68Rmd2xGdSdmPkHfm5TkLIPaAzbGUjN0rwqh5jMBszVME5jECs49gsS72Fi5X3nvMpliPwaLzGe1kXPT_9f8BKViSeA
Cites_doi	10.1016/j.cub.2024.01.067 10.1074/jbc.272.10.6752 10.1093/brain/awu022 10.1016/j.celrep.2023.112231 10.1186/s13229-018-0204-x 10.1159/000478725 10.1038/mp.2013.102 10.1073/pnas.2023674118 10.3389/fncom.2022.849323 10.1038/nrd4627 10.2165/00003495-199448020-00009 10.1007/s10571-018-0632-3 10.1016/j.tips.2013.12.002 10.3371/CSRP.6.3.5 10.1038/nprot.2010.140 10.1016/j.abb.2010.12.017 10.1111/j.1439-0310.1961.tb00217.x 10.1186/s12864-021-08238-1 10.1038/mp.2017.16 10.1093/hmg/ddr212 10.1111/j.1469-7610.2008.01952.x 10.1038/nature12111 10.1172/JCI127411 10.1016/j.neuropharm.2007.02.002
ContentType	Journal Article
Copyright	2025 Copyright © 2025. Published by Elsevier Inc.
Copyright_xml	– notice: 2025 – notice: Copyright © 2025. Published by Elsevier Inc.
DBID	AAYXX CITATION NPM 7X8
DOI	10.1016/j.pnpbp.2025.111528
DatabaseName	CrossRef PubMed MEDLINE - Academic
DatabaseTitle	CrossRef PubMed MEDLINE - Academic
DatabaseTitleList	PubMed MEDLINE - Academic
Database_xml	– sequence: 1 dbid: NPM name: PubMed url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: 7X8 name: MEDLINE - Academic url: https://search.proquest.com/medline sourceTypes: Aggregation Database
DeliveryMethod	fulltext_linktorsrc
Discipline	Pharmacy, Therapeutics, & Pharmacology
EISSN	1878-4216
ExternalDocumentID	41101580 10_1016_j_pnpbp_2025_111528 S0278584625002829
Genre	Journal Article
GroupedDBID	--- --K --M .~1 0R~ 123 1B1 1RT 1~. 1~5 4.4 457 4G. 5RE 7-5 71M 8P~ 9JM AABNK AAEDT AAEDW AAIKJ AAKOC AALRI AAOAW AAQFI AATTM AAXKI AAXLA AAXUO AAYWO ABCQJ ABFRF ABIVO ABJNI ABMAC ABZDS ACDAQ ACGFO ACGFS ACIUM ACLOT ACRLP ACVFH ADBBV ADCNI ADEZE AEBSH AEFWE AEIPS AEKER AENEX AEUPX AFJKZ AFPUW AFTJW AFXIZ AGHFR AGUBO AGWIK AGYEJ AIEXJ AIGII AIIUN AIKHN AITUG AKBMS AKRWK AKYEP ALCLG ALMA_UNASSIGNED_HOLDINGS AMRAJ ANKPU APXCP AXJTR BKOJK BLXMC C45 CS3 DU5 EBS EFJIC EFKBS EFLBG EO8 EO9 EP2 EP3 F5P FDB FIRID FNPLU FYGXN G-Q GBLVA IHE J1W KOM L7B MO0 MOBAO N9A O-L O9- OAUVE OGGZJ OVD OZT P-8 P-9 P2P PC. Q38 ROL RPZ SCC SDF SDG SES SEW SSN SSP SSZ T5K TEORI ~G- ~HD .GJ 29P 53G 5VS 9DU AAQXK AAYXX ABWVN ABXDB ACRPL ADMUD ADNMO AGQPQ AHHHB ASPBG AVWKF AZFZN CITATION EJD FEDTE FGOYB G-2 HMQ HMT HVGLF HZ~ M2V M34 M41 R2- SNS SPT WUQ ZGI NPM 7X8
ID	FETCH-LOGICAL-c311t-fe0b991d5825e3adb694a28a06505b02d3c2af39ec8a6ded14afd639d57e0593
ISICitedReferencesCount	0
ISICitedReferencesURI	http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=001614168700001&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
ISSN	0278-5846 1878-4216
IngestDate	Sat Oct 18 23:54:26 EDT 2025 Fri Nov 07 01:56:23 EST 2025 Sat Nov 29 06:54:47 EST 2025 Wed Dec 10 14:25:54 EST 2025
IsPeerReviewed	true
IsScholarly	true
Keywords	AB mpf slc6a3 Behavioural analysis ARRIVE KO Zebrafish model HPLC-ECD IHC Autism spectrum disorder SD RNA-seq TH ANOVA Dopamine dysfunction IACUC qPCR SEM dpf DA ELISA ISH
Language	English
License	Copyright © 2025. Published by Elsevier Inc.
LinkModel	OpenURL
MergedId	FETCHMERGED-LOGICAL-c311t-fe0b991d5825e3adb694a28a06505b02d3c2af39ec8a6ded14afd639d57e0593
Notes	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
PMID	41101580
PQID	3262228482
PQPubID	23479
ParticipantIDs	proquest_miscellaneous_3262228482 pubmed_primary_41101580 crossref_primary_10_1016_j_pnpbp_2025_111528 elsevier_sciencedirect_doi_10_1016_j_pnpbp_2025_111528
PublicationCentury	2000
PublicationDate	2025-12-20
PublicationDateYYYYMMDD	2025-12-20
PublicationDate_xml	– month: 12 year: 2025 text: 2025-12-20 day: 20
PublicationDecade	2020
PublicationPlace	England
PublicationPlace_xml	– name: England
PublicationTitle	Progress in neuro-psychopharmacology & biological psychiatry
PublicationTitleAlternate	Prog Neuropsychopharmacol Biol Psychiatry
PublicationYear	2025
Publisher	Elsevier Inc
Publisher_xml	– name: Elsevier Inc
References	Pavăl (bb0115) 2017; 39 Kühme (bb0075) 1961; 18 Meltzer (bb0090) 2012; 6 Grant, Fitton (bb0040) 1994; 48 Krantz, Peter, Liu, Edwards (bb0070) 1997; 272 Ng, Zhen, Meyer, Erreger, Li, Kakar, Ahmad, Thiele, Kubisch, Rider, Holmes Morton, Strauss, Puffenberger, D’Agnano, Anikster, Carducci, Hyland, Rotstein, Leuzzi, Borck, Reith, Kurian (bb0105) 2014; 137 Mendonça, da Silva, Hernandez, Castela, Obeso, Costa (bb0095) 2024; 34 DiCarlo, Mabry, Cao, McMillan, Woynaroski, Harrison, Reddy, Matthies, Flynn, Wallace, Wu, Galli (bb0030) 2021; 12 Hamilton, Campbell, Sharma, Erreger, Herborg Hansen, Saunders, Belovich, NIH ARRA Autism Sequencing Consortium, Sahai, Cook, Gether, McHaourab, Matthies, Sutcliffe, Galli (bb0045) 2013; 18 Klein, Battagello, Cardoso, Hauser, Bittencourt, Correa (bb0065) 2019; 39 Ogi, Licitra, Naef, Marchese, Fronte, Gazzano, Santorelli (bb0110) 2020; 7 Jia, He, Yang, Shuai, Yang, Wu, Liu, Chen, Wang, Wang, Song, Ding, Zhu, Zhang, Chen, Qin (bb0055) 2021; 118 Weiss, Tzavara, Davis, Nomikos, Michael McIntosh, Giros, Martres (bb0150) 2007; 52 Gadow, Roohi, DeVincent, Hatchwell (bb0035) 2008; 49 MacRae, Peterson (bb0085) 2015; 14 (bb0135) 2025 Liu, Li, Hu, Wang, Lin, Jiang, Li, Xu (bb0080) 2018; 9 Uribe-Salazar, Kaya, Sekar, Weyenberg, Ingamells, Dennis (bb0120) 2022; 23 Véronneau-Veilleux, Robaey, Ursino, Nekka (bb0130) 2022; 16 (bb0010) 2025 Kalueff, Stewart, Gerlai (bb0060) 2014; 35 . Howe, Clark, Torroja, Torrance, Berthelot, Muffato, Collins, Humphray, McLaren, Matthews, McLaren, Sealy, Caccamo, Churcher, Scott, Barrett, Koch, Rauch, White, Chow, Kilian, Quintais, Guerra-Assunção, Zhou, Gu, Yen, Vogel, Eyre, Redmond, Banerjee, Chi, Fu, Langley, Maguire, Laird, Lloyd, Kenyon, Donaldson, Sehra, Almeida-King, Loveland, Trevanion, Jones, Quail, Willey, Hunt, Burton, Sims, McLay, Plumb, Davis, Clee, Oliver, Clark, Riddle, Elliot, Threadgold, Harden, Ware, Begum, Mortimore, Kerry, Heath, Phillimore, Tracey, Corby, Dunn, Johnson, Wood, Clark, Pelan, Griffiths, Smith, Glithero, Howden, Barker, Lloyd, Stevens, Harley, Holt, Panagiotidis, Lovell, Beasley, Henderson, Gordon, Auger, Wright, Collins, Raisen, Dyer, Leung, Robertson, Ambridge, Leongamornlert, McGuire, Gilderthorp, Griffiths, Manthravadi, Nichol, Barker, Whitehead, Kay, Brown, Murnane, Gray, Humphries, Sycamore, Barker, Saunders, Wallis, Babbage, Hammond, Mashreghi-Mohammadi, Barr, Martin, Wray, Ellington, Matthews, Ellwood, Woodmansey, Clark, Cooper, Tromans, Grafham, Skuce, Pandian, Andrews, Harrison, Kimberley, Garnett, Fosker, Hall, Garner, Kelly, Bird, Palmer, Gehring, Berger, Dooley, Ersan-Ürün, Eser, Geiger, Geisler, Karotki, Kirn, Konantz, Konantz, Oberländer, Rudolph-Geiger, Teucke, Lanz, Raddatz, Osoegawa, Zhu, Rapp, Widaa, Langford, Yang, Schuster, Carter, Harrow, Ning, Herrero, Searle, Enright, Geisler, Plasterk, Lee, Westerfield, de Jong, Zon, Postlethwait, Nüsslein-Volhard, Hubbard, Roest Crollius, Rogers, Stemple (bb0050) 2013; 496 Vidyadhara, Somayaji, Wade, Yücel, Zhao, Shashaank, Ribaudo, Gupta, Lam, Sames, Greene, Sulzer, Chandra (bb0140) 2023; 42 Wang, McCoy, Rodriguiz, Pan, Je, Roberts, Kim, Berrios, Colvin, Bousquet-Moore, Lorenzo, Wu, Weinberg, Ehlers, Philpot, Beaudet, Wetsel, Jiang (bb0145) 2011; 20 DiCarlo, Aguilar, Matthies, Harrison, Bundschuh, West, Hashemi, Herborg, Rickhag, Chen, Gether, Wallace, Galli (bb0025) 2019; 129 Daubner, Le, Wang (bb0020) 2011; 508 Ashok, Marques, Jauhar, Nour, Goodwin, Young, Howes (bb0005) 2017; 22 Jonathan Cachat, Adam Stewart, Leah Grossman, Siddharth Gaikwad, Ferdous Kadri, Kyung Min Chung, Nadine Wu, Keith Wong, Sudipta Roy, Christopher Suciu, Jason Goodspeed, Marco Elegante, Brett Bartels, Salem Elkhayat, David Tien, Julia Tan, Ashley Denmark, Thomas Gilder, Evan Kyzar, John Dileo, Kevin Frank, Katie Chang, Eli Utterback, Peter Hart, Allan V Kalueff.,2010.Measuring behavioral and endocrine responses to novelty stress in adult zebrafish 5(11):1786-99. doi Autism-linked dopamine transporter mutation alters striatal dopamine neurotransmission and dopamine-dependent behaviors - PubMed [WWW Document], 2025. (accessed 9.8.24). URL Véronneau-Veilleux, Robaey, Ursino, Nekka (bb0125) 2022; 16 Uribe-Salazar (10.1016/j.pnpbp.2025.111528_bb0120) 2022; 23 Ng (10.1016/j.pnpbp.2025.111528_bb0105) 2014; 137 Mendonça (10.1016/j.pnpbp.2025.111528_bb0095) 2024; 34 10.1016/j.pnpbp.2025.111528_bb0100 Liu (10.1016/j.pnpbp.2025.111528_bb0080) 2018; 9 DiCarlo (10.1016/j.pnpbp.2025.111528_bb0025) 2019; 129 Krantz (10.1016/j.pnpbp.2025.111528_bb0070) 1997; 272 Véronneau-Veilleux (10.1016/j.pnpbp.2025.111528_bb0125) 2022; 16 Jia (10.1016/j.pnpbp.2025.111528_bb0055) 2021; 118 DiCarlo (10.1016/j.pnpbp.2025.111528_bb0030) 2021; 12 10.1016/j.pnpbp.2025.111528_bb0015 Howe (10.1016/j.pnpbp.2025.111528_bb0050) 2013; 496 Wang (10.1016/j.pnpbp.2025.111528_bb0145) 2011; 20 Daubner (10.1016/j.pnpbp.2025.111528_bb0020) 2011; 508 Kalueff (10.1016/j.pnpbp.2025.111528_bb0060) 2014; 35 Vidyadhara (10.1016/j.pnpbp.2025.111528_bb0140) 2023; 42 Gadow (10.1016/j.pnpbp.2025.111528_bb0035) 2008; 49 Hamilton (10.1016/j.pnpbp.2025.111528_bb0045) 2013; 18 Véronneau-Veilleux (10.1016/j.pnpbp.2025.111528_bb0130) 2022; 16 (10.1016/j.pnpbp.2025.111528_bb0135) 2025 Grant (10.1016/j.pnpbp.2025.111528_bb0040) 1994; 48 Ashok (10.1016/j.pnpbp.2025.111528_bb0005) 2017; 22 Weiss (10.1016/j.pnpbp.2025.111528_bb0150) 2007; 52 Klein (10.1016/j.pnpbp.2025.111528_bb0065) 2019; 39 Meltzer (10.1016/j.pnpbp.2025.111528_bb0090) 2012; 6 MacRae (10.1016/j.pnpbp.2025.111528_bb0085) 2015; 14 Ogi (10.1016/j.pnpbp.2025.111528_bb0110) 2020; 7 Pavăl (10.1016/j.pnpbp.2025.111528_bb0115) 2017; 39 Kühme (10.1016/j.pnpbp.2025.111528_bb0075) 1961; 18
References_xml	– volume: 18 start-page: 1315 year: 2013 end-page: 1323 ident: bb0045 article-title: De novo mutation in the dopamine transporter gene associates dopamine dysfunction with autism spectrum disorder publication-title: Mol. Psychiatry – volume: 23 start-page: 12 year: 2022 ident: bb0120 article-title: Evaluation of CRISPR gene-editing tools in zebrafish publication-title: BMC Genomics – volume: 42 year: 2023 ident: bb0140 article-title: Dopamine transporter and synaptic vesicle sorting defects underlie auxilin-associated Parkinson’s disease publication-title: Cell Rep. – volume: 39 start-page: 31 year: 2019 end-page: 59 ident: bb0065 article-title: Dopamine: functions, signaling, and association with neurological diseases publication-title: Cell. Mol. Neurobiol. – volume: 118 year: 2021 ident: bb0055 article-title: A pair of dopamine neurons mediate chronic stress signals to induce learning deficit in Drosophila melanogaster publication-title: Proc. Natl. Acad. Sci. U. S. A. – volume: 35 start-page: 63 year: 2014 end-page: 75 ident: bb0060 article-title: Zebrafish as an emerging model for studying complex brain disorders publication-title: Trends Pharmacol. Sci. – volume: 16 year: 2022 ident: bb0130 article-title: A mechanistic model of ADHD as resulting from dopamine phasic/tonic imbalance during reinforcement learning publication-title: Front. Comput. Neurosci. – volume: 9 start-page: 23 year: 2018 ident: bb0080 article-title: CRISPR/Cas9-induced shank3b mutant zebrafish display autism-like behaviors publication-title: Mol. Autism. – volume: 129 start-page: 3407 year: 2019 end-page: 3419 ident: bb0025 article-title: Autism-linked dopamine transporter mutation alters striatal dopamine neurotransmission and dopamine-dependent behaviors publication-title: J. Clin. Invest. – volume: 137 start-page: 1107 year: 2014 end-page: 1119 ident: bb0105 article-title: Dopamine transporter deficiency syndrome: phenotypic spectrum from infancy to adulthood publication-title: Brain – volume: 508 start-page: 1 year: 2011 end-page: 12 ident: bb0020 article-title: Tyrosine hydroxylase and regulation of dopamine synthesis publication-title: Arch. Biochem. Biophys. – reference: Autism-linked dopamine transporter mutation alters striatal dopamine neurotransmission and dopamine-dependent behaviors - PubMed [WWW Document], 2025. (accessed 9.8.24). URL – volume: 20 start-page: 3093 year: 2011 end-page: 3108 ident: bb0145 article-title: Synaptic dysfunction and abnormal behaviors in mice lacking major isoforms of Shank3 publication-title: Hum. Mol. Genet. – volume: 22 start-page: 666 year: 2017 end-page: 679 ident: bb0005 article-title: The dopamine hypothesis of bipolar affective disorder: the state of the art and implications for treatment publication-title: Mol. Psychiatry – volume: 7 year: 2020 ident: bb0110 article-title: Social preference tests in zebrafish: a systematic review publication-title: Front Vet Sci – volume: 6 start-page: 134 year: 2012 end-page: 144 ident: bb0090 article-title: Clozapine: balancing safety with superior antipsychotic efficacy publication-title: Clin. Schizophr. Relat. Psychoses – volume: 49 start-page: 1331 year: 2008 end-page: 1338 ident: bb0035 article-title: Association of ADHD, tics, and anxiety with dopamine transporter (DAT1) genotype in autism spectrum disorder publication-title: J. Child Psychol. Psychiatry – volume: 12 year: 2021 ident: bb0030 article-title: Autism-associated variant in the SLC6A3 gene alters the oral microbiome and metabolism in a murine model publication-title: Front. Psych. – volume: 272 start-page: 6752 year: 1997 end-page: 6759 ident: bb0070 article-title: Phosphorylation of a vesicular monoamine transporter by casein kinase II publication-title: J. Biol. Chem. – reference: Jonathan Cachat, Adam Stewart, Leah Grossman, Siddharth Gaikwad, Ferdous Kadri, Kyung Min Chung, Nadine Wu, Keith Wong, Sudipta Roy, Christopher Suciu, Jason Goodspeed, Marco Elegante, Brett Bartels, Salem Elkhayat, David Tien, Julia Tan, Ashley Denmark, Thomas Gilder, Evan Kyzar, John Dileo, Kevin Frank, Katie Chang, Eli Utterback, Peter Hart, Allan V Kalueff.,2010.Measuring behavioral and endocrine responses to novelty stress in adult zebrafish 5(11):1786-99. doi: – year: 2025 ident: bb0135 publication-title: Vesicular monoamine transporter 2 (SLC18A2) regulates monoamine turnover and brain development in zebrafish - PubMed [WWW Document] – reference: . – volume: 496 start-page: 498 year: 2013 end-page: 503 ident: bb0050 article-title: The zebrafish reference genome sequence and its relationship to the human genome publication-title: Nature – volume: 39 start-page: 355 year: 2017 end-page: 360 ident: bb0115 article-title: A dopamine hypothesis of autism Spectrum disorder publication-title: Dev. Neurosci. – volume: 52 start-page: 1496 year: 2007 end-page: 1508 ident: bb0150 article-title: Functional alterations of nicotinic neurotransmission in dopamine transporter knock-out mice publication-title: Neuropharmacology – volume: 16 year: 2022 ident: bb0125 article-title: A mechanistic model of ADHD as resulting from dopamine phasic/tonic imbalance during reinforcement learning publication-title: Front. Comput. Neurosci. – volume: 18 start-page: 33 year: 1961 end-page: 55 ident: bb0075 article-title: Verhaltensstudien am maulbrütenden ( publication-title: Z. Tierpsychol. – volume: 14 start-page: 721 year: 2015 end-page: 731 ident: bb0085 article-title: Zebrafish as tools for drug discovery publication-title: Nat. Rev. Drug Discov. – volume: 48 start-page: 253 year: 1994 end-page: 273 ident: bb0040 article-title: Risperidone. A review of its pharmacology and therapeutic potential in the treatment of schizophrenia publication-title: Drugs – volume: 34 start-page: 1034 year: 2024 end-page: 1047.e4 ident: bb0095 article-title: Dopamine neuron activity encodes the length of upcoming contralateral movement sequences publication-title: Curr. Biol. – year: 2025 ident: bb0010 article-title: Assessing Social Behavior Phenotypes in Adult Zebrafish: Shoaling, Social Preference, and Mirror Biting Tests \| SpringerLink – volume: 7 year: 2020 ident: 10.1016/j.pnpbp.2025.111528_bb0110 article-title: Social preference tests in zebrafish: a systematic review publication-title: Front Vet Sci – volume: 34 start-page: 1034 year: 2024 ident: 10.1016/j.pnpbp.2025.111528_bb0095 article-title: Dopamine neuron activity encodes the length of upcoming contralateral movement sequences publication-title: Curr. Biol. doi: 10.1016/j.cub.2024.01.067 – volume: 272 start-page: 6752 year: 1997 ident: 10.1016/j.pnpbp.2025.111528_bb0070 article-title: Phosphorylation of a vesicular monoamine transporter by casein kinase II publication-title: J. Biol. Chem. doi: 10.1074/jbc.272.10.6752 – volume: 137 start-page: 1107 year: 2014 ident: 10.1016/j.pnpbp.2025.111528_bb0105 article-title: Dopamine transporter deficiency syndrome: phenotypic spectrum from infancy to adulthood publication-title: Brain doi: 10.1093/brain/awu022 – volume: 42 year: 2023 ident: 10.1016/j.pnpbp.2025.111528_bb0140 article-title: Dopamine transporter and synaptic vesicle sorting defects underlie auxilin-associated Parkinson’s disease publication-title: Cell Rep. doi: 10.1016/j.celrep.2023.112231 – volume: 9 start-page: 23 year: 2018 ident: 10.1016/j.pnpbp.2025.111528_bb0080 article-title: CRISPR/Cas9-induced shank3b mutant zebrafish display autism-like behaviors publication-title: Mol. Autism. doi: 10.1186/s13229-018-0204-x – year: 2025 ident: 10.1016/j.pnpbp.2025.111528_bb0135 – volume: 39 start-page: 355 year: 2017 ident: 10.1016/j.pnpbp.2025.111528_bb0115 article-title: A dopamine hypothesis of autism Spectrum disorder publication-title: Dev. Neurosci. doi: 10.1159/000478725 – volume: 18 start-page: 1315 year: 2013 ident: 10.1016/j.pnpbp.2025.111528_bb0045 article-title: De novo mutation in the dopamine transporter gene associates dopamine dysfunction with autism spectrum disorder publication-title: Mol. Psychiatry doi: 10.1038/mp.2013.102 – volume: 118 year: 2021 ident: 10.1016/j.pnpbp.2025.111528_bb0055 article-title: A pair of dopamine neurons mediate chronic stress signals to induce learning deficit in Drosophila melanogaster publication-title: Proc. Natl. Acad. Sci. U. S. A. doi: 10.1073/pnas.2023674118 – volume: 16 year: 2022 ident: 10.1016/j.pnpbp.2025.111528_bb0125 article-title: A mechanistic model of ADHD as resulting from dopamine phasic/tonic imbalance during reinforcement learning publication-title: Front. Comput. Neurosci. doi: 10.3389/fncom.2022.849323 – volume: 14 start-page: 721 year: 2015 ident: 10.1016/j.pnpbp.2025.111528_bb0085 article-title: Zebrafish as tools for drug discovery publication-title: Nat. Rev. Drug Discov. doi: 10.1038/nrd4627 – volume: 48 start-page: 253 year: 1994 ident: 10.1016/j.pnpbp.2025.111528_bb0040 article-title: Risperidone. A review of its pharmacology and therapeutic potential in the treatment of schizophrenia publication-title: Drugs doi: 10.2165/00003495-199448020-00009 – volume: 39 start-page: 31 year: 2019 ident: 10.1016/j.pnpbp.2025.111528_bb0065 article-title: Dopamine: functions, signaling, and association with neurological diseases publication-title: Cell. Mol. Neurobiol. doi: 10.1007/s10571-018-0632-3 – volume: 35 start-page: 63 year: 2014 ident: 10.1016/j.pnpbp.2025.111528_bb0060 article-title: Zebrafish as an emerging model for studying complex brain disorders publication-title: Trends Pharmacol. Sci. doi: 10.1016/j.tips.2013.12.002 – volume: 12 year: 2021 ident: 10.1016/j.pnpbp.2025.111528_bb0030 article-title: Autism-associated variant in the SLC6A3 gene alters the oral microbiome and metabolism in a murine model publication-title: Front. Psych. – volume: 16 year: 2022 ident: 10.1016/j.pnpbp.2025.111528_bb0130 article-title: A mechanistic model of ADHD as resulting from dopamine phasic/tonic imbalance during reinforcement learning publication-title: Front. Comput. Neurosci. doi: 10.3389/fncom.2022.849323 – volume: 6 start-page: 134 year: 2012 ident: 10.1016/j.pnpbp.2025.111528_bb0090 article-title: Clozapine: balancing safety with superior antipsychotic efficacy publication-title: Clin. Schizophr. Relat. Psychoses doi: 10.3371/CSRP.6.3.5 – ident: 10.1016/j.pnpbp.2025.111528_bb0100 doi: 10.1038/nprot.2010.140 – ident: 10.1016/j.pnpbp.2025.111528_bb0015 – volume: 508 start-page: 1 year: 2011 ident: 10.1016/j.pnpbp.2025.111528_bb0020 article-title: Tyrosine hydroxylase and regulation of dopamine synthesis publication-title: Arch. Biochem. Biophys. doi: 10.1016/j.abb.2010.12.017 – volume: 18 start-page: 33 year: 1961 ident: 10.1016/j.pnpbp.2025.111528_bb0075 article-title: Verhaltensstudien am maulbrütenden (Betta anabatoides Bleeker) und am nestbauenden Kampffisch (B. splendens Regan)* publication-title: Z. Tierpsychol. doi: 10.1111/j.1439-0310.1961.tb00217.x – volume: 23 start-page: 12 year: 2022 ident: 10.1016/j.pnpbp.2025.111528_bb0120 article-title: Evaluation of CRISPR gene-editing tools in zebrafish publication-title: BMC Genomics doi: 10.1186/s12864-021-08238-1 – volume: 22 start-page: 666 year: 2017 ident: 10.1016/j.pnpbp.2025.111528_bb0005 article-title: The dopamine hypothesis of bipolar affective disorder: the state of the art and implications for treatment publication-title: Mol. Psychiatry doi: 10.1038/mp.2017.16 – volume: 20 start-page: 3093 year: 2011 ident: 10.1016/j.pnpbp.2025.111528_bb0145 article-title: Synaptic dysfunction and abnormal behaviors in mice lacking major isoforms of Shank3 publication-title: Hum. Mol. Genet. doi: 10.1093/hmg/ddr212 – volume: 49 start-page: 1331 year: 2008 ident: 10.1016/j.pnpbp.2025.111528_bb0035 article-title: Association of ADHD, tics, and anxiety with dopamine transporter (DAT1) genotype in autism spectrum disorder publication-title: J. Child Psychol. Psychiatry doi: 10.1111/j.1469-7610.2008.01952.x – volume: 496 start-page: 498 year: 2013 ident: 10.1016/j.pnpbp.2025.111528_bb0050 article-title: The zebrafish reference genome sequence and its relationship to the human genome publication-title: Nature doi: 10.1038/nature12111 – volume: 129 start-page: 3407 year: 2019 ident: 10.1016/j.pnpbp.2025.111528_bb0025 article-title: Autism-linked dopamine transporter mutation alters striatal dopamine neurotransmission and dopamine-dependent behaviors publication-title: J. Clin. Invest. doi: 10.1172/JCI127411 – volume: 52 start-page: 1496 year: 2007 ident: 10.1016/j.pnpbp.2025.111528_bb0150 article-title: Functional alterations of nicotinic neurotransmission in dopamine transporter knock-out mice publication-title: Neuropharmacology doi: 10.1016/j.neuropharm.2007.02.002
SSID	ssj0001303
Score	2.466764
Snippet	Dopamine plays a crucial role in regulating movement, motivation, attention, and emotions. Disruptions in dopamine metabolism have been linked to various...
SourceID	proquest pubmed crossref elsevier
SourceType	Aggregation Database Index Database Publisher
StartPage	111528
SubjectTerms	Autism spectrum disorder Behavioural analysis Dopamine dysfunction slc6a3 Zebrafish model
Title	Dopamine disruption and autism phenotypes in slc6a3−/− zebrafish: Behavioural and molecular insights
URI	https://dx.doi.org/10.1016/j.pnpbp.2025.111528 https://www.ncbi.nlm.nih.gov/pubmed/41101580 https://www.proquest.com/docview/3262228482
Volume	143
WOSCitedRecordID	wos001614168700001&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
hasFullText	1
inHoldings	1
isFullTextHit
isPrint
journalDatabaseRights	– providerCode: PRVESC databaseName: Elsevier SD Freedom Collection Journals 2021 customDbUrl: eissn: 1878-4216 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0001303 issn: 0278-5846 databaseCode: AIEXJ dateStart: 19950101 isFulltext: true titleUrlDefault: https://www.sciencedirect.com providerName: Elsevier
link	http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV3Nb9MwFLe6jgMXxDdjMBmJ7bIaGifOB7eJtQI0lR6CyC1yYgc6rWnWrNPGnf-bZztOOlVD48DFqiz5Q_n9-vze83vPCL3lhRfyIc0AAVoQzw04yfxCEplD6zPJnIDrxyaCySRMkmja6_22uTCXZ0FZhldXUfVfoYY-AFulzv4D3O2k0AG_AXRoAXZo7wT8MZjBc6U7ilm9XBmJoCuywpr1_FDFdC2U41UHwtZnuc9dAquRw1_qDrmY1T-Vl6Cpm6iLcqjRc_uMropeVwZ9va7WTlWUl5KZMKWukElMdlfVFcY2kTmm5pMmxs04ax0UpCMLvnfJaa03O5nxBRjuP7pblJXtvl61vltTDmHS8L1xZVCmwkLocE36hmDSetRpamNv9m3Ie-N6OH1XlVWmqo9Sps4A1uSb36iuPfmajr-dnKTxKIn33XF1TtTTY-qKft89NjTYQts0YFHYR9tHn0fJl_ZIVwe9stztXmz5Kh0ouLH2bSrObSaMVmXih-hBY4PgI8OdR6gny8foYGqwuh7guMvJqwf4AE_XUHyChCUY7giGgSLYEAx3BMOzEjcEe09wS68PeI1cemRLLmzJ9RTF41H88RNp3uogues4F6SQwwxMDcFCyqTLReZHHqcgBsACYNmQCjenvHAjmYfcF1I4Hi8EaMeCBVK9KvkM9ctFKV8gzAPpuVkhqOShx0TAC-ZFoELlEWjqMMsOGtivm1amIktqQxVPUw1GqsBIDRg7yLcIpI1SaZTFFDj094FvLF4piFx1j8ZLuVjVKVg8ym_qhbCX5wbIdiceqNMOC4cv7zB6F93v_gWvUP9iuZKv0b38EtBa7qGtIAn3Gib-AWCPsGM
linkProvider	Elsevier
openUrl	ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Dopamine+disruption+and+autism+phenotypes+in+slc6a3-%2F-+zebrafish%3A+Behavioural+and+molecular+insights&rft.jtitle=Progress+in+neuro-psychopharmacology+%26+biological+psychiatry&rft.au=Li%2C+Wen&rft.au=Zhang%2C+Xiaocong&rft.au=Niu%2C+Xiaoyu&rft.au=Qin%2C+Nan&rft.date=2025-12-20&rft.issn=1878-4216&rft.eissn=1878-4216&rft_id=info:doi/10.1016%2Fj.pnpbp.2025.111528&rft.externalDBID=NO_FULL_TEXT
thumbnail_l	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0278-5846&client=summon
thumbnail_m	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0278-5846&client=summon
thumbnail_s	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0278-5846&client=summon