Epithelial-mesenchymal interactions control basement membrane production and differentiation in cultured and transplanted mouse keratinocytes

The effects of mesenchyme and substratum on epidermal differentiation and formation of a basement membrane (BM) were analyzed in vitro and in vivo. Primary epidermal cell cultures (PEC) from neonatal mice were grown: on plastic culture dishes; on lifted collagen gels, either alone or in recombinatio...

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Veröffentlicht in:Cell and tissue research Jg. 244; H. 2; S. 413
Hauptverfasser: Bohnert, A, Hornung, J, Mackenzie, I C, Fusenig, N E
Format: Journal Article
Sprache:Englisch
Veröffentlicht: Germany 01.01.1986
Schlagworte:
Animals
Basement Membrane - analysis
Basement Membrane - ultrastructure
Cell Differentiation
Cells, Cultured
Collagen
Culture Techniques - instrumentation
Epidermal Cells
Epidermis - metabolism
Epidermis - transplantation
Extracellular Matrix - analysis
Gels
Keratins - analysis
Mice
Mice, Inbred C3H
Microscopy, Electron
ISSN:0302-766X
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Zusammenfassung:The effects of mesenchyme and substratum on epidermal differentiation and formation of a basement membrane (BM) were analyzed in vitro and in vivo. Primary epidermal cell cultures (PEC) from neonatal mice were grown: on plastic culture dishes; on lifted collagen gels, either alone or in recombination with mesenchyme; after reimplantation in vivo either directly on mesenchyme or on collagen interposed between keratinocytes and mesenchyme. Differentiation of the epithelium and formation of a BM were examined by electron microscopy, and expression of BM constituents (type IV collagen, laminin, fibronectin, bullous pemphigoid antigen, and heparan sulfate proteoglycan) by indirect immunofluorescence. PEC on plastic or on collagen gels showed poor differentiation, a structured BM was not visible, and the expression and deposition of BM constituents was incomplete. Upon reimplantation in vivo, differentiation was normalized, expression of BM components complete and a structured BM reformed. This effect does not depend on immediate contact of epidermal cells with mesenchyme. When PEC on collagen gel were similarly associated with dermal mesenchyme in vitro, epidermal differentiation and expression of BM components were almost normalized, but a structured BM was absent. These findings demonstrate that formation of the BM in epidermis is a function of keratinocytes and, like differentiation is subject to mesenchymal control. A structural BM is not a prerequisite but rather an additional criterion of normal epidermal differentiation.
Bibliographie:ObjectType-Article-1
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content type line 23
ISSN:0302-766X
DOI:10.1007/BF00219217