Evaluation of bone regeneration using human derived-gingival mesenchymal stem cells loaded on beta tricalcium phosphate and hyaluronic acid: an experimental study
Background Healing of critical-sized bone defects (CSDs) is a challenging problem in both clinical and research settings. Aim The present study aimed to assess the regenerative capacity of human gingival-derived mesenchymal stem cells (hGMSCs) loaded on beta-tricalcium phosphate scaffold (β-TCP) and...
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| Veröffentlicht in: | Tanta Dental Journal Jg. 21; H. 1; S. 60 - 65 |
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| Hauptverfasser: | , , , , |
| Format: | Journal Article |
| Sprache: | Englisch |
| Veröffentlicht: |
India
Wolters Kluwer - Medknow
2024
Medknow Publications and Media Pvt. Ltd |
| Ausgabe: | 2 |
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| ISSN: | 1687-8574, 2536-9644 |
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| Abstract | Background
Healing of critical-sized bone defects (CSDs) is a challenging problem in both clinical and research settings.
Aim
The present study aimed to assess the regenerative capacity of human gingival-derived mesenchymal stem cells (hGMSCs) loaded on beta-tricalcium phosphate scaffold (β-TCP) and hyaluronic acid (HA) gel in surgically created standardized CSDs in rabbit's femurs.
Materials and methods
To achieve this aim, CSDs of 6 mm diameter each, were unilaterally created in femur of adult New Zeeland male white rabbits (n = 18). The rabbits were then divided randomly into three groups and received the following treatment modalities: group A (study group): six defects were treated with hGMSCs loaded on β-TCP scaffold combined with HA gel; group B (positive control group): six defects (n = 6 rabbits) were treated with β-TCP combined with HA gel; group C (negative control group): three defects were left without intervention. Two rabbits from groups A, B and one rabbit from group C were sacrificed at 6 weeks, femurs were dissected out to evaluate bone healing histologically and histomorphometrically.
Results
The findings of this study indicate that, hGMSCs exhibited fibroblast like morphology and expressed phenotypic MSCs markers (positive for cluster of differentiation CD105 and negative for CD34). Histologically, local application of hGMSCs loaded on β-TCP scaffold with HA gel showed enhanced pattern of bone regeneration as compared to the unloaded scaffold. Histomorphometrically, there was a statistically significant difference in the newly formed bone between the bony defects treated with hGMSCs and control groups.
Conclusion
GMSCs can be considered as a dependent source of MSCs with bone tissue regenerative capacity. |
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| AbstractList | Background
Healing of critical-sized bone defects (CSDs) is a challenging problem in both clinical and research settings.
Aim
The present study aimed to assess the regenerative capacity of human gingival-derived mesenchymal stem cells (hGMSCs) loaded on beta-tricalcium phosphate scaffold (β-TCP) and hyaluronic acid (HA) gel in surgically created standardized CSDs in rabbit's femurs.
Materials and methods
To achieve this aim, CSDs of 6 mm diameter each, were unilaterally created in femur of adult New Zeeland male white rabbits (n = 18). The rabbits were then divided randomly into three groups and received the following treatment modalities: group A (study group): six defects were treated with hGMSCs loaded on β-TCP scaffold combined with HA gel; group B (positive control group): six defects (n = 6 rabbits) were treated with β-TCP combined with HA gel; group C (negative control group): three defects were left without intervention. Two rabbits from groups A, B and one rabbit from group C were sacrificed at 6 weeks, femurs were dissected out to evaluate bone healing histologically and histomorphometrically.
Results
The findings of this study indicate that, hGMSCs exhibited fibroblast like morphology and expressed phenotypic MSCs markers (positive for cluster of differentiation CD105 and negative for CD34). Histologically, local application of hGMSCs loaded on β-TCP scaffold with HA gel showed enhanced pattern of bone regeneration as compared to the unloaded scaffold. Histomorphometrically, there was a statistically significant difference in the newly formed bone between the bony defects treated with hGMSCs and control groups.
Conclusion
GMSCs can be considered as a dependent source of MSCs with bone tissue regenerative capacity. |
| Audience | Academic |
| Author | Badr, Ahamed M. Sarhan, Naglaa I. Abo-Zaid, Mohammed Abd-ElFattah Alkaleny, Heba H. El Razzak, Mona Y. A. |
| Author_xml | – sequence: 1 givenname: Mohammed Abd-ElFattah surname: Abo-Zaid fullname: Abo-Zaid, Mohammed Abd-ElFattah email: Mohammed_abozaid@dent.tanta.edu.eg – sequence: 2 givenname: Mona Y. A. surname: El Razzak fullname: El Razzak, Mona Y. A. – sequence: 3 givenname: Naglaa I. surname: Sarhan fullname: Sarhan, Naglaa I. – sequence: 4 givenname: Heba H. surname: Alkaleny fullname: Alkaleny, Heba H. – sequence: 5 givenname: Ahamed M. surname: Badr fullname: Badr, Ahamed M. |
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| Cites_doi | 10.1007/s11302-020-09703-4 10.1186/1471-2288-4-26 10.1080/14653240600855905 10.1038/ijos.2014.6 10.1016/S0003-9969(02)00119-X 10.1111/j.1600-0501.2011.02193.x 10.1016/j.biomaterials.2006.10.015 10.22203/eCM.v038a12 10.3349/ymj.2003.44.2.187 10.1016/j.tice.2019.101325 10.1016/j.jbiomech.2022.111078 10.1016/j.bone.2004.07.002 10.1902/jop.2004.75.9.1281 |
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| References | Carluccio (R4-20240909) 2020; 16 Behnia (R15-20240909) 2013; 115 Freeman (R2-20240909) 2019; 38 Al-Qadhi (R8-20240909) 2020; 63 Huo (R3-20240909) 2022; 136 Hernandez (R14-20240909) 2004; 35 Zhu (R1-20240909) 2021; 6 Yuan (R11-20240909) 2007; 28 Delgado-Ruiz (R7-20240909) 2014; 6 Lu (R9-20240909) 2002; 47 Dominici (R13-20240909) 2006; 8 Kawaguchi (R10-20240909) 2004; 75 Calvo-Guirado (R6-20240909) 2012; 23 Saghaei (R5-20240909) 2004; 4 Kim (R12-20240909) 2003; 44 |
| References_xml | – volume: 16 start-page: 263 year: 2020 ident: R4-20240909 article-title: Adult mesenchymal stem cells:Is there a role for purine receptors in their osteogenic differentiation? publication-title: Purinergic Signal doi: 10.1007/s11302-020-09703-4 – volume: 4 start-page: 1 year: 2004 ident: R5-20240909 article-title: Random allocation software for parallel group randomized trials publication-title: BMC Med Res Methodol doi: 10.1186/1471-2288-4-26 – volume: 8 start-page: 315 year: 2006 ident: R13-20240909 article-title: Minimal criteria for defining multipotent mesenchymal stromal cells. The international society for cellular therapy position statement publication-title: Cytotherapy doi: 10.1080/14653240600855905 – volume: 6 start-page: 105 year: 2014 ident: R7-20240909 article-title: Porous titanium granules in critical size defects of rabbit tibia with or without membranes publication-title: Int J Oral Sci doi: 10.1038/ijos.2014.6 – volume: 47 start-page: 831 year: 2002 ident: R9-20240909 article-title: The effect of demineralized intramembranous bone matrix and basic fibroblast growth factor on the healing of allogeneic intramembranous bone grafts in the rabbit publication-title: Arch Oral Biol doi: 10.1016/S0003-9969(02)00119-X – volume: 6 start-page: 4110 year: 2021 ident: R1-20240909 article-title: Bone physiological microenvironment and healing mechanism:Basis for future bone-tissue engineering scaffolds publication-title: Bioact Mater – volume: 23 start-page: 667 year: 2012 ident: R6-20240909 article-title: Retracted:histomorphometric and mineral degradation study of ossceram:a novel biphasic b-tricalcium phosphate, in critical size defects in rabbits publication-title: Clin Oral Implants Res doi: 10.1111/j.1600-0501.2011.02193.x – volume: 28 start-page: 1005 year: 2007 ident: R11-20240909 article-title: Repair of canine mandibular bone defects with bone marrow stromal cells and porous β-tricalcium phosphate publication-title: Biomaterials doi: 10.1016/j.biomaterials.2006.10.015 – volume: 115 start-page: 7 year: 2013 ident: R15-20240909 article-title: Bone regeneration with a combination of nanocrystallinehydroxyapatite silica gel, platelet-rich growth factor, and mesenchymalstem cells:a histologic study in rabbit calvaria publication-title: Oral Surgery – volume: 38 start-page: 168 year: 2019 ident: R2-20240909 article-title: Biofabrication of multiscale bone extracellular matrix scaffolds for bone tissue engineering publication-title: Eur Cells Mater doi: 10.22203/eCM.v038a12 – volume: 44 start-page: 187 year: 2003 ident: R12-20240909 article-title: Interaction of mesenchymal stem cells and osteoblasts for in vitro osteogenesis publication-title: Yonsei Med J doi: 10.3349/ymj.2003.44.2.187 – volume: 63 start-page: 1 year: 2020 ident: R8-20240909 article-title: Gingival mesenchymal stem cells as an alternative source to bone marrow mesenchymal stem cells in regeneration of bone defects:in vivo study publication-title: Tissue and Cell doi: 10.1016/j.tice.2019.101325 – volume: 136 start-page: 111078 year: 2022 ident: R3-20240909 article-title: Simulation on bone remodeling with stochastic nature of adult and elderly using topology optimization algorithm publication-title: J Biomech doi: 10.1016/j.jbiomech.2022.111078 – volume: 35 start-page: 1095 year: 2004 ident: R14-20240909 article-title: Osteocyte density in woven bone publication-title: Bone doi: 10.1016/j.bone.2004.07.002 – volume: 75 start-page: 1281 year: 2004 ident: R10-20240909 article-title: Enhancement of periodontal tissue regeneration by transplantation of bone marrow mesenchymal stem cells publication-title: J Periodontol doi: 10.1902/jop.2004.75.9.1281 |
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Healing of critical-sized bone defects (CSDs) is a challenging problem in both clinical and research settings.
Aim
The present study aimed to assess... |
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| SubjectTerms | Analysis Calcium phosphate Hyaluronic acid Original article : Oral Biology, Oral Pathology, Oral and Maxillofacial Surgery, Oral Medicine, Periodontology and Oral Radiology Stem cells Transmission Control Protocol/Internet Protocol (Computer network protocol) |
| Title | Evaluation of bone regeneration using human derived-gingival mesenchymal stem cells loaded on beta tricalcium phosphate and hyaluronic acid: an experimental study |
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