Effect of brain-derived neurotrophic factor on c-jun expression in the rd mouse retina

AIM:To determine the location of c-jun protein,dynamic changes in c-jun mRNA and protein expression,and ultrastructure characteristics in the rd mouse retina,following a single dose of brain-derived neurotrophic factor(BDNF) in a short period of time.· METHODS:A single intravitreal injection of BDNF...

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Published in:International journal of ophthalmology Vol. 5; no. 3; pp. 266 - 271
Main Authors: Chen, Rui, Yin, Xiao-Bei, Peng, Chun-Xia, Li, Gen-Lin
Format: Journal Article
Language:English
Published: China International Journal of Ophthalmology Press 2012
Subjects:
Basic Research
brain-derived
c-jun
ERK
factor
neurotrophic
photoreceptor
c-jun
BDNF
retina
photoreceptor
ISSN:2222-3959, 2227-4898, 2227-4898
Online Access:Get full text
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Summary:AIM:To determine the location of c-jun protein,dynamic changes in c-jun mRNA and protein expression,and ultrastructure characteristics in the rd mouse retina,following a single dose of brain-derived neurotrophic factor(BDNF) in a short period of time.· METHODS:A single intravitreal injection of BDNF at two dosages(25μg/L or 50μg/L) was given to the right eye of the rd mouse at age 2 and 3 weeks respectively.Two weeks after injection,the location of c-jun protein in the retina was observed by immunofluorescence detection,c-jun mRNA and protein expression in retinas were detected by quantitative real time polymerase chain reaction(RT-PCR) and western immunoblotting analysis,ultrastructure characteristics of retinas were detected by transmission electron microscope(TEM) observation.· RESULTS:c-jun protein was expressed in the inner nuclear layer(INL) of retina.BDNF at two dosages(25μg/L and 50μg/L) increased c-jun mRNA expression at PN-4 weeks respectively(P1 =0.019,P2=0.021).50μg/L BDNF increased c-jun protein expression at PN-4 weeks(P =0.000).The retinal ultrastructure was improved.· CONCLUSION:The effects of BDNF exerts on the c-jun expression in the retina are dose-dependent and time-dependent,which may mediate photoreceptor rescue indirectly in the pathological process of retinitis pigmentosa(RP)at early stage.
Bibliography:Rui Chen,Xiao-Bei Yin,Chun-Xia Peng,Gen-Lin Li Beijing Tongren Eye Center,Beijing Tongren Hospital,Capital Medical University,Beijing Key Laboratory of Ophthalmology and Visual Sciences,Beijing 100730,China
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ISSN:2222-3959
2227-4898
2227-4898
DOI:10.3980/j.issn.2222-3959.2012.03.03