Rodent Heart and Brain Tissue Preparation for Digital Macro Photography after Ischemia-reperfusion

Macro photography is applicable for imaging various tissue samples at high magnification to perform qualitative and quantitative analyses. Tissue preparation and subsequent image capture are steps performed immediately after the ischemia-reperfusion (IR) experiment and must be performed in a timely...

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Bibliographic Details
Published in:Journal of visualized experiments no. 180
Main Authors: Liepinsh, Edgars, Kuka, Janis, Zvejniece, Liga, Vilskersts, Reinis, Dambrova, Maija
Format: Journal Article
Language:English
Published: United States 01.02.2022
Subjects:
Animals
brain
Brain - diagnostic imaging
cameras
chlorides
heart
Image Processing, Computer-Assisted - methods
Ischemia
lighting
photography
Photography - methods
Rats
Reperfusion
Rodentia
ISSN:1940-087X, 1940-087X
Online Access:Get full text
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Summary:Macro photography is applicable for imaging various tissue samples at high magnification to perform qualitative and quantitative analyses. Tissue preparation and subsequent image capture are steps performed immediately after the ischemia-reperfusion (IR) experiment and must be performed in a timely manner and with appropriate care. For the evaluation of IR-induced damage in the heart and brain, this paper describes 2,3,5-triphenyl-2H-tetrazolium chloride (TTC)-based staining followed by macro photography. Scientific macro photography requires controlled lighting and an appropriate imaging setup. The standardized methodology ensures high-quality, detailed digital images even if a combination of an inexpensive up-to-date digital camera and macro lens is used. Proper techniques and potential mistakes in sample preparation and image acquisition are discussed, and examples of the influence of correct and incorrect setups on image quality are provided. Specific tips are provided on how to avoid common mistakes, such as overstaining, improper sample storage, and suboptimal lighting conditions. This paper shows the appropriate methodology for rat heart and brain tissue slicing and staining and provides guidelines for establishing lighting and camera setups and photography techniques for high-resolution image acquisition.
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ISSN:1940-087X
1940-087X
DOI:10.3791/62942