Preparation of Leukocyte-Poor Platelet Concentrates via a Short, Hard Spin of a Pool of Buffy Coats

Background and Objectives: A new method for the preparation of leukocyte‐poor platelet concentrates was developed, based on a short, hard spin of a pool of 5 buffy coats (BCs) combined with automated collection of the platelets. Materials and Methods: The characteristics of platelet concentrates (PC...

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Published in:Vox sanguinis Vol. 78; no. 3; pp. 164 - 170
Main Authors: van Delden, C.J., Faber, R.D., de Wit, H.J.C., Sibinga, C. Th. Smit
Format: Journal Article
Language:English
Published: Oxford, UK Blackwell Science Ltd 01.04.2000
Blackwell
S. Karger AG
Subjects:
Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy
Biological and medical sciences
Blood Component Removal - methods
Blood Component Removal - standards
Blood Platelets - cytology
Blood. Blood and plasma substitutes. Blood products. Blood cells. Blood typing. Plasmapheresis. Apheresis
Centrifugation
Humans
Hydrogen-Ion Concentration
Leukocyte Count
Leukocytes
Medical sciences
Platelet Count
Time Factors
Transfusions. Complications. Transfusion reactions. Cell and gene therapy
Human
Blood cell
Platelet
Blood substitute
Preparation
Concentrate
Buffer system
Technique
White cell reduction
ISSN:0042-9007, 1423-0410
Online Access:Get full text
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Summary:Background and Objectives: A new method for the preparation of leukocyte‐poor platelet concentrates was developed, based on a short, hard spin of a pool of 5 buffy coats (BCs) combined with automated collection of the platelets. Materials and Methods: The characteristics of platelet concentrates (PCs) were studied as a function of the total g force applied to a pool of 5 BCs. Pools of BCs were centrifuged for 1 min with a total g force ranging from about 3,300 to 5,000 gmin (n = 7–9 per applied g force). Deceleration took place without the means of a brake. The total centrifugation time was about 11 min. The platelet‐rich plasma (PRP) fraction above the cell layer was separated by an automated component preparation device. Results: A short, hard spin with a total g force of between 3,400 and 4,600 gmin resulted in PCs that contained on average more than 290×109 platelets and less than 5×106 leukocytes without the use of a leukocyte filter, provided that the transfer of PRP was electronically checked and terminated. The cell concentrations in the PCs are a function of the total g force. Both the platelet and leukocyte levels in the concentrate decreased with an increase in the total g force applied to the pool. Conclusion: The preparation of PCs via a short hard, spin of BCs, combined with automated collection of the PRP molecular may be an alternative method for the preparation of leukocyte‐poor PCs.
Bibliography:istex:2526E1573425C34523498308A7F944483414A83E
ark:/67375/WNG-4GD8L4T9-Z
ArticleID:VOXVOX783_0164
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 14
ISSN:0042-9007
1423-0410
DOI:10.1046/j.1423-0410.2000.7830164.x