Efficacy of chitosan nanoparticles and mesenchymal stem cells in rabbit models for sciatic nerve regeneration

Peripheral nerve injuries (PNI) are frequently caused by several circumstances, including trauma and iatrogenic operations, and there are limited therapy options to help with functional recovery. This study aimed to compare in vivo adipose-derived stem cells (ASCs) to chitosan nanoparticles (CS NPs)...

Celý popis

Uloženo v:
Podrobná bibliografie
Vydáno v:Iraqi journal of veterinary sciences Ročník 38; číslo 2; s. 369 - 377
Hlavní autoři: Al-Haideri, Dhurgham H., Al-Timmemi, Hameed A.
Médium: Journal Article
Jazyk:angličtina
Vydáno: University of Mosul, College of Veterinary Medicine 01.04.2024
Témata:
ascs
conduit
cs nps
pni
rabbit
ISSN:2071-1255, 1607-3894, 2071-1255
On-line přístup:Získat plný text
Tagy: Přidat tag
Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!
Abstract Peripheral nerve injuries (PNI) are frequently caused by several circumstances, including trauma and iatrogenic operations, and there are limited therapy options to help with functional recovery. This study aimed to compare in vivo adipose-derived stem cells (ASCs) to chitosan nanoparticles (CS NPs) in rabbit sciatic nerve defect regeneration. Twenty healthy rabbits of local breed randomly enrolled in two equal groups split into two groups at random: The critical size defect was bridged with a 14 mm conduit from acellular bovine urinary bladder impacted with chitosan nanoparticles (CS NPs) semi-gel 1mg/ml as the first group, and the critical size defect was bridged with mesenchymal stem cells (MSCs) 5x106 in the second group. At the end of 56 and 112 days, assessment of the nerve regeneration, motor and sensory function, and histopathological assessment of the sciatic nerve were investigated. According to the findings, the motor and sensory capabilities of the sciatic nerve increased faster in the mesenchymal stem cells (MSCs) group. In the mesenchymal stem cells group repairing a 10mm sciatic nerve defect, histopathological examination revealed basophilic nuclei of proliferated Schwann cells with thick myelin sheath, suitable orientation nerve fibers, dense axon population, large axon diameter, nerve fiber myelination, and low fibrous tissue at the epineurium compared to chitosan nanoparticles (CS NPs). It is concluded that effect mesenchymal stem cells (MSCs) promote regeneration of the sciatic nerve defect compared to chitosan nanoparticles (CS NPs).
AbstractList Peripheral nerve injuries (PNI) are frequently caused by several circumstances, including trauma and iatrogenic operations, and there are limited therapy options to help with functional recovery. This study aimed to compare in vivo adipose-derived stem cells (ASCs) to chitosan nanoparticles (CS NPs) in rabbit sciatic nerve defect regeneration. Twenty healthy rabbits of local breed randomly enrolled in two equal groups split into two groups at random: The critical size defect was bridged with a 14 mm conduit from acellular bovine urinary bladder impacted with chitosan nanoparticles (CS NPs) semi-gel 1mg/ml as the first group, and the critical size defect was bridged with mesenchymal stem cells (MSCs) 5x106 in the second group. At the end of 56 and 112 days, assessment of the nerve regeneration, motor and sensory function, and histopathological assessment of the sciatic nerve were investigated. According to the findings, the motor and sensory capabilities of the sciatic nerve increased faster in the mesenchymal stem cells (MSCs) group. In the mesenchymal stem cells group repairing a 10mm sciatic nerve defect, histopathological examination revealed basophilic nuclei of proliferated Schwann cells with thick myelin sheath, suitable orientation nerve fibers, dense axon population, large axon diameter, nerve fiber myelination, and low fibrous tissue at the epineurium compared to chitosan nanoparticles (CS NPs). It is concluded that effect mesenchymal stem cells (MSCs) promote regeneration of the sciatic nerve defect compared to chitosan nanoparticles (CS NPs).
Author Al-Timmemi, Hameed A.
Al-Haideri, Dhurgham H.
Author_xml – sequence: 1
  givenname: Dhurgham H.
  surname: Al-Haideri
  fullname: Al-Haideri, Dhurgham H.
– sequence: 2
  givenname: Hameed A.
  surname: Al-Timmemi
  fullname: Al-Timmemi, Hameed A.
BookMark eNqFkc1KBDEQhIMoqKuvIHmBXfMzSSbgRcQ_ELzoOfT0JJplJpFkEPbtnd1VEC_2pZuCKrr4TslhyskTcsHZSsrW2su4_qwrwYRc8UYoI1aSt_qAnAhm-JILpQ5_3cfkvNY1245h2qgTMt6GEBFwQ3Og-B6nXCHRBCl_QJkiDr5SSD0dffUJ3zcjDLROfqToh6HSmGiBrosTHXPvZyHkQitGmK00-fLpafFvfr5mJaczchRgqP78ey_I693ty83D8un5_vHm-mmJom30UkjbG446CAkYdOjawDigbE3PdWC9MIKhV8Lavm1n2SptsWPSB9vyRqJckMd9bp9h7T5KHKFsXIbodkIub-67nZOBNV3odSNBN1ZxMEaDZKhUhyhkM2dd7bOw5FqLDw7jtGszFYiD48ztSLgtCbcl4fYk3JbEbNd_7D_v_GP8AmyBkcY
CitedBy_id crossref_primary_10_1177_19373341251376279
crossref_primary_10_3390_ijms252413302
crossref_primary_10_1590_1519_6984_280569
ContentType Journal Article
DBID AAYXX
CITATION
DOA
DOI 10.33899/ijvs.2023.142572.3186
DatabaseName CrossRef
DOAJ Directory of Open Access Journals
DatabaseTitle CrossRef
DatabaseTitleList
Database_xml – sequence: 1
  dbid: DOA
  name: DOAJ Directory of Open Access Journals
  url: https://www.doaj.org/
  sourceTypes: Open Website
DeliveryMethod fulltext_linktorsrc
Discipline Veterinary Medicine
EISSN 2071-1255
EndPage 377
ExternalDocumentID oai_doaj_org_article_3f04bfd643a64951a776a30c55bcc234
10_33899_ijvs_2023_142572_3186
GroupedDBID .K5
5VS
AAYXX
ABDBF
ADBBV
ALMA_UNASSIGNED_HOLDINGS
BAWUL
BCNDV
CITATION
DIK
DYU
ESX
GROUPED_DOAJ
KQ8
N.T
OK1
OZF
ID FETCH-LOGICAL-c2846-239d71c6f23acf6fb8f01ac387d16f0d2720ce5299d88c389569cb03ef98143c3
IEDL.DBID DOA
ISSN 2071-1255
1607-3894
IngestDate Fri Oct 03 12:39:31 EDT 2025
Tue Nov 18 22:27:12 EST 2025
Sat Nov 29 04:46:28 EST 2025
IsDoiOpenAccess true
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Issue 2
Language English
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-c2846-239d71c6f23acf6fb8f01ac387d16f0d2720ce5299d88c389569cb03ef98143c3
OpenAccessLink https://doaj.org/article/3f04bfd643a64951a776a30c55bcc234
PageCount 9
ParticipantIDs doaj_primary_oai_doaj_org_article_3f04bfd643a64951a776a30c55bcc234
crossref_citationtrail_10_33899_ijvs_2023_142572_3186
crossref_primary_10_33899_ijvs_2023_142572_3186
PublicationCentury 2000
PublicationDate 2024-04-01
PublicationDateYYYYMMDD 2024-04-01
PublicationDate_xml – month: 04
  year: 2024
  text: 2024-04-01
  day: 01
PublicationDecade 2020
PublicationTitle Iraqi journal of veterinary sciences
PublicationYear 2024
Publisher University of Mosul, College of Veterinary Medicine
Publisher_xml – name: University of Mosul, College of Veterinary Medicine
SSID ssj0000070675
ssib044742163
Score 2.332732
Snippet Peripheral nerve injuries (PNI) are frequently caused by several circumstances, including trauma and iatrogenic operations, and there are limited therapy...
SourceID doaj
crossref
SourceType Open Website
Enrichment Source
Index Database
StartPage 369
SubjectTerms ascs
conduit
cs nps
pni
rabbit
Title Efficacy of chitosan nanoparticles and mesenchymal stem cells in rabbit models for sciatic nerve regeneration
URI https://doaj.org/article/3f04bfd643a64951a776a30c55bcc234
Volume 38
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
journalDatabaseRights – providerCode: PRVAON
  databaseName: DOAJ Directory of Open Access Journals
  customDbUrl:
  eissn: 2071-1255
  dateEnd: 99991231
  omitProxy: false
  ssIdentifier: ssj0000070675
  issn: 2071-1255
  databaseCode: DOA
  dateStart: 20050101
  isFulltext: true
  titleUrlDefault: https://www.doaj.org/
  providerName: Directory of Open Access Journals
– providerCode: PRVHPJ
  databaseName: ROAD: Directory of Open Access Scholarly Resources
  customDbUrl:
  eissn: 2071-1255
  dateEnd: 99991231
  omitProxy: false
  ssIdentifier: ssib044742163
  issn: 2071-1255
  databaseCode: M~E
  dateStart: 19880101
  isFulltext: true
  titleUrlDefault: https://road.issn.org
  providerName: ISSN International Centre
link http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV09T8MwELVQxcCCoIAoX_LAGjWJHScZAbVioBUDVN0i-xJDEU1RUip14bdz54SqWxeWDFZsWe9OuXvx3TNjtzmIsIDCeBKM8KRQhZdCpL3AIBfIA99ETq5p8hSPx8l0mj5vXfVFNWGNPHADXF9YXxqbY-DUCpP5QMex0sKHKDIAoXBKoH6cbpEp9CQpkfEFrbBUkwjHlBoT-1L0Yy5JZdMuLEhfrj_7WJF0dyjww4E-HCKTpebqrUi1JejvIs_wiB22KSO_a7Z6zPZ01WXdCdWxuGZaPmrPx0_YfECKEBrWfGE5nRAsal3yUpdIjdsKOK7LnM-p5wje13Ncl5ScOf2_r_ms5JU2Zrbk7oKcmmNGy2tnPeAl1UbyqnhzQtVkz1P2Ohy8PDx67YUKHmAUUl4o0jwOQNlQaLDKmsT6gQaRxHmgrJ_TmSwUEUaoPElwGLlTCsYXhU0TzKtAnLFOuSiLc8bRAqGPbASzQ41GFYm0SqcgpJVahbHpsegPvAxatXG69OIzQ9bhQM8I9IxAzxrQMwK9x_qbeV-N3sbOGfdkm83bpJftBtCLshbbbJcXXfzHIpfsADfX1vVcsc6y-i6u2T6slrO6unEOis_Rz-AXreHm7Q
linkProvider Directory of Open Access Journals
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Efficacy+of+chitosan+nanoparticles+and+mesenchymal+stem+cells+in+rabbit+models+for+sciatic+nerve+regeneration&rft.jtitle=Iraqi+journal+of+veterinary+sciences&rft.au=Dhurgham+H.+Al-Haideri&rft.au=Hameed+A.+Al-Timmemi&rft.date=2024-04-01&rft.pub=University+of+Mosul%2C+College+of+Veterinary+Medicine&rft.issn=1607-3894&rft.eissn=2071-1255&rft.volume=38&rft.issue=2&rft.spage=369&rft.epage=377&rft_id=info:doi/10.33899%2Fijvs.2023.142572.3186&rft.externalDBID=DOA&rft.externalDocID=oai_doaj_org_article_3f04bfd643a64951a776a30c55bcc234
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2071-1255&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2071-1255&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2071-1255&client=summon