Analysis of the Stromal Cellular Components of the Solid Tumor Microenvironment Using Flow Cytometry

The tumor microenvironment consists of a variety of cell types. The contribution of each cell type to the tumor is an emerging subject in the field of cancer research. Here, we describe protocols for dissociating tumor tissues and Matrigel plugs into single cells for further analysis by flow cytomet...

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Bibliographic Details
Published in:	Current protocols in cell biology Vol. 70; p. 19.18.1
Main Authors:	Timaner, Michael, Beyar-Katz, Ofrat, Shaked, Yuval
Format:	Journal Article
Language:	English
Published:	United States 01.03.2016
Subjects:	Animals Breast - cytology Breast - pathology Breast Neoplasms - pathology Cell Separation - methods Collagen - chemistry Colon - cytology Colon - pathology Colonic Neoplasms - pathology Drug Combinations Female Flow Cytometry - methods Humans Laminin - chemistry Mice Proteoglycans - chemistry Stromal Cells - pathology Tumor Microenvironment cell dissociation host bone marrow derived cells cancer cells
ISSN:	1934-2616, 1934-2616
Online Access:	Get more information
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