Analysis of the Stromal Cellular Components of the Solid Tumor Microenvironment Using Flow Cytometry

The tumor microenvironment consists of a variety of cell types. The contribution of each cell type to the tumor is an emerging subject in the field of cancer research. Here, we describe protocols for dissociating tumor tissues and Matrigel plugs into single cells for further analysis by flow cytomet...

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Vydané v:	Current protocols in cell biology Ročník 70; s. 19.18.1
Hlavní autori:	Timaner, Michael, Beyar-Katz, Ofrat, Shaked, Yuval
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	United States 01.03.2016
Predmet:	Animals Breast - cytology Breast - pathology Breast Neoplasms - pathology Cell Separation - methods Collagen - chemistry Colon - cytology Colon - pathology Colonic Neoplasms - pathology Drug Combinations Female Flow Cytometry - methods Humans Laminin - chemistry Mice Proteoglycans - chemistry Stromal Cells - pathology Tumor Microenvironment cell dissociation host bone marrow derived cells cancer cells
ISSN:	1934-2616, 1934-2616
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Abstract	The tumor microenvironment consists of a variety of cell types. The contribution of each cell type to the tumor is an emerging subject in the field of cancer research. Here, we describe protocols for dissociating tumor tissues and Matrigel plugs into single cells for further analysis by flow cytometry. These protocols can be used for evaluating the cellular component of solid tumors from human or mouse origin or Matrigel plugs implanted in mice. The protocols describe the dissociation of tumor tissue with or without dissociation automatic devices. Subsequently, the use of flow cytometry for immunophenotypic analysis of host cells found in the tumor microenvironment, including myeloid derived suppressor cells, endothelial cells, and macrophages is provided. These methods can be used to broaden our understanding of the cross-talk between tumor and host cells in the tumor microenvironment. © 2016 by John Wiley & Sons, Inc.
AbstractList	The tumor microenvironment consists of a variety of cell types. The contribution of each cell type to the tumor is an emerging subject in the field of cancer research. Here, we describe protocols for dissociating tumor tissues and Matrigel plugs into single cells for further analysis by flow cytometry. These protocols can be used for evaluating the cellular component of solid tumors from human or mouse origin or Matrigel plugs implanted in mice. The protocols describe the dissociation of tumor tissue with or without dissociation automatic devices. Subsequently, the use of flow cytometry for immunophenotypic analysis of host cells found in the tumor microenvironment, including myeloid derived suppressor cells, endothelial cells, and macrophages is provided. These methods can be used to broaden our understanding of the cross-talk between tumor and host cells in the tumor microenvironment. © 2016 by John Wiley & Sons, Inc. The tumor microenvironment consists of a variety of cell types. The contribution of each cell type to the tumor is an emerging subject in the field of cancer research. Here, we describe protocols for dissociating tumor tissues and Matrigel plugs into single cells for further analysis by flow cytometry. These protocols can be used for evaluating the cellular component of solid tumors from human or mouse origin or Matrigel plugs implanted in mice. The protocols describe the dissociation of tumor tissue with or without dissociation automatic devices. Subsequently, the use of flow cytometry for immunophenotypic analysis of host cells found in the tumor microenvironment, including myeloid derived suppressor cells, endothelial cells, and macrophages is provided. These methods can be used to broaden our understanding of the cross-talk between tumor and host cells in the tumor microenvironment. © 2016 by John Wiley & Sons, Inc.The tumor microenvironment consists of a variety of cell types. The contribution of each cell type to the tumor is an emerging subject in the field of cancer research. Here, we describe protocols for dissociating tumor tissues and Matrigel plugs into single cells for further analysis by flow cytometry. These protocols can be used for evaluating the cellular component of solid tumors from human or mouse origin or Matrigel plugs implanted in mice. The protocols describe the dissociation of tumor tissue with or without dissociation automatic devices. Subsequently, the use of flow cytometry for immunophenotypic analysis of host cells found in the tumor microenvironment, including myeloid derived suppressor cells, endothelial cells, and macrophages is provided. These methods can be used to broaden our understanding of the cross-talk between tumor and host cells in the tumor microenvironment. © 2016 by John Wiley & Sons, Inc.
Author	Timaner, Michael Beyar-Katz, Ofrat Shaked, Yuval
Author_xml	– sequence: 1 givenname: Michael surname: Timaner fullname: Timaner, Michael organization: Department of Cell Biology and Cancer Science, Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa, Israel – sequence: 2 givenname: Ofrat surname: Beyar-Katz fullname: Beyar-Katz, Ofrat organization: Department of Cell Biology and Cancer Science, Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa, Israel – sequence: 3 givenname: Yuval surname: Shaked fullname: Shaked, Yuval organization: Department of Cell Biology and Cancer Science, Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa, Israel
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Title	Analysis of the Stromal Cellular Components of the Solid Tumor Microenvironment Using Flow Cytometry
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