Analysis of the Stromal Cellular Components of the Solid Tumor Microenvironment Using Flow Cytometry

The tumor microenvironment consists of a variety of cell types. The contribution of each cell type to the tumor is an emerging subject in the field of cancer research. Here, we describe protocols for dissociating tumor tissues and Matrigel plugs into single cells for further analysis by flow cytomet...

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Bibliographic Details
Published in:Current protocols in cell biology Vol. 70; p. 19.18.1
Main Authors: Timaner, Michael, Beyar-Katz, Ofrat, Shaked, Yuval
Format: Journal Article
Language:English
Published: United States 01.03.2016
Subjects:
Animals
Breast - cytology
Breast - pathology
Breast Neoplasms - pathology
Cell Separation - methods
Collagen - chemistry
Colon - cytology
Colon - pathology
Colonic Neoplasms - pathology
Drug Combinations
Female
Flow Cytometry - methods
Humans
Laminin - chemistry
Mice
Proteoglycans - chemistry
Stromal Cells - pathology
Tumor Microenvironment
cell dissociation
host
bone marrow derived cells
cancer cells
ISSN:1934-2616, 1934-2616
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Summary:The tumor microenvironment consists of a variety of cell types. The contribution of each cell type to the tumor is an emerging subject in the field of cancer research. Here, we describe protocols for dissociating tumor tissues and Matrigel plugs into single cells for further analysis by flow cytometry. These protocols can be used for evaluating the cellular component of solid tumors from human or mouse origin or Matrigel plugs implanted in mice. The protocols describe the dissociation of tumor tissue with or without dissociation automatic devices. Subsequently, the use of flow cytometry for immunophenotypic analysis of host cells found in the tumor microenvironment, including myeloid derived suppressor cells, endothelial cells, and macrophages is provided. These methods can be used to broaden our understanding of the cross-talk between tumor and host cells in the tumor microenvironment. © 2016 by John Wiley & Sons, Inc.
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ISSN:1934-2616
1934-2616
DOI:10.1002/0471143030.cb1918s70