Identification of Periopathogenes from Dental Plaque in Periodontal Patients with PCR Technique and Their Association with Composite Interleukin-1 Genotype

The present study aimed to assess the presence of main types of microorganisms involved in the aetiopathogenesis of chronic periodontitis with PCR technique and determinates the presence of composite IL-1 genotype and their associations with founded bacteria. The examined group was consisted from 20...

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Bibliographic Details
Published in:Prilozi (Makedonska akademija na naukite i umetnostite. Oddelenie za medicinski nauki) Vol. 40; no. 2; pp. 89 - 97
Main Authors: Stojanovska, Aneta Atanasovska, Todoroska, Saska, Popovska, Mirjana, Muratovska, Ilijana, Bedzeti, Linda Zendeli
Format: Journal Article
Language:English
Published: North Macedonia De Gruyter Brill Sp. z o.o., Paradigm Publishing Services 01.10.2019
Subjects:
Adult
Aggregatibacter actinomycetemcomitans - genetics
Aggregatibacter actinomycetemcomitans - growth & development
Aggregatibacter actinomycetemcomitans - isolation & purification
Chronic Periodontitis - metabolism
Chronic Periodontitis - microbiology
Cytokines
Dental plaque
Dental Plaque - metabolism
Dental Plaque - microbiology
Dental Plaque Index
Genotype
Genotype & phenotype
Gum disease
Humans
Interleukin-1 - genetics
Middle Aged
Periodontal Index
Periodontal Pocket - microbiology
Polymerase Chain Reaction - methods
Porphyromonas gingivalis - genetics
Porphyromonas gingivalis - growth & development
Porphyromonas gingivalis - isolation & purification
Prevotella intermedia - genetics
Prevotella intermedia - growth & development
Prevotella intermedia - isolation & purification
Treponema denticola - genetics
Treponema denticola - growth & development
Treponema denticola - isolation & purification
periopathogenes
periodontitis
composite genotype
ISSN:1857-8985, 1857-8985, 0350-1914
Online Access:Get full text
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Summary:The present study aimed to assess the presence of main types of microorganisms involved in the aetiopathogenesis of chronic periodontitis with PCR technique and determinates the presence of composite IL-1 genotype and their associations with founded bacteria. The examined group was consisted from 20 subjects with diagnosed chronic periodontitis and 20 healthy control without periodontitis. Clinical parameters like gingival index (GI), plaque index (PI), bleeding on probing (BOP), periodontal pocket depth (PPD) and clinical attachment lost (CAL) were determinates. Subgingival dental plaque was collected using a sterilized paper point. We used Parodontose Plus test, reverse hybridization kit, for the detection of periodontal marker bacteria, as well as for the detection of composite Interleukin -1 Genotype Results: The most present bacterial species detected from subgingival dental plaque was Treponema denticola and Porfiromonas gingivalis which was present in 65% of examined patients. In relation to the presence of positive genotype in patients, there was no significant difference between the test and control group for p> 0.05 (p = 1.00). For χ2=8,17 (p=0,06, p<0,05) there is an association between Prevotella intermedia, and composite genotype. Between positive genotype and analyzed bacterial species A. actinomycetem comitans for p> 0.05 (p = 1.00), P. gingivalis for p> 0.05 (p = 0.16), T. Forsythia for p> 0.05 (p = 0.20), T. Denticola for p> 0.05 (p = 0.64) no association was found. This investigations confirmed the strong association of these five examined periopathogenes with periodontitis.
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ISSN:1857-8985
1857-8985
0350-1914
DOI:10.2478/prilozi-2019-0019