Amperometric Bioelectronic Tongue for glucose determination

An amperometric Bioelectronic Tongue is reported for glucose determination that contains eight sensor electrodes constructed using different metal electrodes (Pt, Au), oxidoreductase enzymes (glucose oxidase, ascorbate oxidase, uricase), and membrane coatings (Nafion, chitosan). The response to vary...

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Bibliographic Details
Published in:Sensing and Bio-Sensing Research Vol. 3; no. C; pp. 31 - 37
Main Authors: Al-Issa, Yazan, Njagi, John, Schuckers, Stephanie C., Suni, Ian I.
Format: Journal Article
Language:English
Published: Elsevier 01.03.2015
Subjects:
Amperometry
Bioelectronic Tongue
Chemical interference
Glucose estimation
Multi-sensor array
Multivariate analysis
ISSN:2214-1804, 2214-1804
Online Access:Get full text
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Summary:An amperometric Bioelectronic Tongue is reported for glucose determination that contains eight sensor electrodes constructed using different metal electrodes (Pt, Au), oxidoreductase enzymes (glucose oxidase, ascorbate oxidase, uricase), and membrane coatings (Nafion, chitosan). The response to varying concentrations of glucose, ascorbic acid, uric acid, and acetaminophen was tested for two models, concentration determination by current density measurements at individual electrodes and concentration determination by a linear regression model for the entire electrode array. The reduced chi-squared for the full array model was found to be about one order of magnitude lower than that for the individual-electrode model. Discrimination of glucose from chemical interference by the other three species is accomplished through a combination of enzyme catalysis, metal electrocatalysis, and membrane surface charge. The benefit of incorporating enzyme electrodes into the sensor array is illustrated by the lower correlation coefficients between different enzyme electrodes relative to non-enzyme coated electrodes. This approach can be more generally applied to detection of other substrates of oxidoreductase enzymes.
ISSN:2214-1804
2214-1804
DOI:10.1016/j.sbsr.2014.10.015