Small Noncoding 4.5SH and 4.5SI RNAs and Their Binding to Proteins

— The functions of small noncoding RNAs 4.5SH and 4.5SI found in murine-like rodents are unclear. These RNAs synthesized by RNA polymerase III are widely expressed in rodent organs and tissues. Using crosslinking assays, it was shown that approximately half of all 4.5SI and 4.5SH RNA molecules were...

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Vydané v:Molecular biology (New York) Ročník 52; číslo 6; s. 899 - 904
Hlavní autori: Tatosyan, K. A., Koval, A. P., Kramerov, D. A.
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: Moscow Pleiades Publishing 01.11.2018
Springer Nature B.V
Predmet:
Biochemistry
Biomedical and Life Sciences
DNA-directed RNA polymerase
Human Genetics
Immunoprecipitation
La protein
Life Sciences
Molecular Cell Biology
Nucleotide sequence
Protein X
Proteins
RNA modification
RNA polymerase
RNA-binding proteins
4.5S RNA
small noncoding RNA
La protein
rodents
ISSN:0026-8933, 1608-3245
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Shrnutí:— The functions of small noncoding RNAs 4.5SH and 4.5SI found in murine-like rodents are unclear. These RNAs synthesized by RNA polymerase III are widely expressed in rodent organs and tissues. Using crosslinking assays, it was shown that approximately half of all 4.5SI and 4.5SH RNA molecules were bound to proteins provisionally called X and Y, respectively. An immunoprecipitation experiment showed that both these RNAs were associated with the La protein, which did not crosslink to them. The termini of 4.5SI RNA form a long duplex stem, which makes the molecule more stable than 4.5SH RNA. Modification of the 5'-end sequence destructing the stem of 4.5SI RNA altered its protein-binding properties; after the 3'-end sequence was changed to the complementary, both the stem structure and the RNA binding to protein X were restored. Presumably, this protein plays a role in increasing the half-life of 4.5SI RNA.
Bibliografia:ObjectType-Article-1
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content type line 14
ISSN:0026-8933
1608-3245
DOI:10.1134/S002689331806016X