A signal on-off ratiometric electrochemical sensing platform coupled with a molecularly imprinted polymer and CuCo2O4/NCNTs signal amplification for selective determination of gemcitabine

A novel and reliable electrochemical analysis tool was developed for gemcitabine (GEM) detection by combining the selective recognition of molecular imprinted polymer (MIP) technology and ratiometric quantification strategy. In this contribution, a glassy carbon electrode (GCE) surface was first mod...

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Vydané v:Sensors and actuators. B, Chemical Ročník 371; s. 132552
Hlavní autori: Hatamluyi, Behnaz, Sadeghzadeh, Samira, Sadeghian, Reihaneh, Mirimoghaddam, Mohammad Mobin, Boroushaki, Mohammad Taher
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: Lausanne Elsevier B.V 15.11.2022
Elsevier Science Ltd
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ISSN:0925-4005, 1873-3077
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Abstract A novel and reliable electrochemical analysis tool was developed for gemcitabine (GEM) detection by combining the selective recognition of molecular imprinted polymer (MIP) technology and ratiometric quantification strategy. In this contribution, a glassy carbon electrode (GCE) surface was first modified by drop-coating CuCo2O4/NCNTs and ferrocene (FC) to achieve a ratiometric on-off response (CuCo2O4/NCNTs/FC was served as a substrate to amplify signal and to provide reference signal). Then, MIP network was prepared through in situ electrochemical polymerization, using aniline as monomer and GEM as template. Differential pulse voltammetry (DPV) analysis showed that the oxidation peak signal of FC decreased with the increase of GEM concentration, whereas the oxidation peak signal of GEM increased. Therefore, the peak current ratio of IGEM/IFC was employed to accurately reflect the concentration of GEM. Using this method, GEM was measured in the range from 0.1 to 150 µM with a low limit of detection of 11.3 nM. In addition, the sensor showed high reproducibility, anti-interference ability and good stability for 6 weeks toward GEM detection. Finally, the proposed sensor exhibited excellent detection performance in complex pharmaceutical and biological matrices with satisfactory recoveries. •A molecular imprinting ratiometric electrochemical sensor is developed for the detection of gemcitabine.•The CuCo2O4/NCNTs nanocomposite is applied to amplify the signal.•The sensor exhibits excellent gemcitabine sensing performance in complex pharmaceutical and biological matrices.
AbstractList A novel and reliable electrochemical analysis tool was developed for gemcitabine (GEM) detection by combining the selective recognition of molecular imprinted polymer (MIP) technology and ratiometric quantification strategy. In this contribution, a glassy carbon electrode (GCE) surface was first modified by drop-coating CuCo2O4/NCNTs and ferrocene (FC) to achieve a ratiometric on-off response (CuCo2O4/NCNTs/FC was served as a substrate to amplify signal and to provide reference signal). Then, MIP network was prepared through in situ electrochemical polymerization, using aniline as monomer and GEM as template. Differential pulse voltammetry (DPV) analysis showed that the oxidation peak signal of FC decreased with the increase of GEM concentration, whereas the oxidation peak signal of GEM increased. Therefore, the peak current ratio of IGEM/IFC was employed to accurately reflect the concentration of GEM. Using this method, GEM was measured in the range from 0.1 to 150 µM with a low limit of detection of 11.3 nM. In addition, the sensor showed high reproducibility, anti-interference ability and good stability for 6 weeks toward GEM detection. Finally, the proposed sensor exhibited excellent detection performance in complex pharmaceutical and biological matrices with satisfactory recoveries. •A molecular imprinting ratiometric electrochemical sensor is developed for the detection of gemcitabine.•The CuCo2O4/NCNTs nanocomposite is applied to amplify the signal.•The sensor exhibits excellent gemcitabine sensing performance in complex pharmaceutical and biological matrices.
A novel and reliable electrochemical analysis tool was developed for gemcitabine (GEM) detection by combining the selective recognition of molecular imprinted polymer (MIP) technology and ratiometric quantification strategy. In this contribution, a glassy carbon electrode (GCE) surface was first modified by drop-coating CuCo2O4/NCNTs and ferrocene (FC) to achieve a ratiometric on-off response (CuCo2O4/NCNTs/FC was served as a substrate to amplify signal and to provide reference signal). Then, MIP network was prepared through in situ electrochemical polymerization, using aniline as monomer and GEM as template. Differential pulse voltammetry (DPV) analysis showed that the oxidation peak signal of FC decreased with the increase of GEM concentration, whereas the oxidation peak signal of GEM increased. Therefore, the peak current ratio of IGEM/IFC was employed to accurately reflect the concentration of GEM. Using this method, GEM was measured in the range from 0.1 to 150 µM with a low limit of detection of 11.3 nM. In addition, the sensor showed high reproducibility, anti-interference ability and good stability for 6 weeks toward GEM detection. Finally, the proposed sensor exhibited excellent detection performance in complex pharmaceutical and biological matrices with satisfactory recoveries.
ArticleNumber 132552
Author Sadeghian, Reihaneh
Hatamluyi, Behnaz
Boroushaki, Mohammad Taher
Sadeghzadeh, Samira
Mirimoghaddam, Mohammad Mobin
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  givenname: Reihaneh
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  fullname: Sadeghian, Reihaneh
  organization: Medical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran
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  givenname: Mohammad Mobin
  surname: Mirimoghaddam
  fullname: Mirimoghaddam, Mohammad Mobin
  organization: Medical Doctor, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
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  givenname: Mohammad Taher
  surname: Boroushaki
  fullname: Boroushaki, Mohammad Taher
  email: Boroushakimt@mums.ac.ir
  organization: Department of Pharmacology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
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Keywords CuCo2O4
Gemcitabine
Ratiometric electrochemical sensor
Nitrogen-doped carbon nanotubes
Molecularly imprinted polymer
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Snippet A novel and reliable electrochemical analysis tool was developed for gemcitabine (GEM) detection by combining the selective recognition of molecular imprinted...
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SubjectTerms Amplification
Aniline
CuCo2O4
Electrochemical analysis
Gemcitabine
Glassy carbon
Imprinted polymers
Molecularly imprinted polymer
Nitrogen-doped carbon nanotubes
Oxidation
Ratiometric electrochemical sensor
Reference signals
Substrates
Title A signal on-off ratiometric electrochemical sensing platform coupled with a molecularly imprinted polymer and CuCo2O4/NCNTs signal amplification for selective determination of gemcitabine
URI https://dx.doi.org/10.1016/j.snb.2022.132552
https://www.proquest.com/docview/2734714291
Volume 371
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