Mediated spectroelectrochemical determination of holo-transferrin reduction potential using a flow cell with disposable screen-printed indium‑tin oxide electrode

A convenient spectroelectrochemical method for the determination of holo-transferrin reduction potential is described. All materials and accessories are commercially available and the results are in agreement with previous reports that required custom-built hardware. The method requires minimal prep...

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Veröffentlicht in:Electrochemistry communications Jg. 84; S. 32 - 35
Hauptverfasser: Weitner, Tin, Biruš, Ivan, Šakić, Davor, Deriš, Helena
Format: Journal Article
Sprache:Englisch
Veröffentlicht: Elsevier B.V 01.11.2017
Schlagworte:
Indium‑tin oxide
Methyl viologen
Reduction potential
Spectroelectrochemistry
Transferrin
Transferrin
Reduction potential
Indium‑tin oxide
Spectroelectrochemistry
Methyl viologen
ISSN:1388-2481, 1873-1902
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Zusammenfassung:A convenient spectroelectrochemical method for the determination of holo-transferrin reduction potential is described. All materials and accessories are commercially available and the results are in agreement with previous reports that required custom-built hardware. The method requires minimal preparation with simple sample handling, small solution volume, cost-effective electrode replacement and automated measurement. This method can easily be applied to the study of similar systems in aqueous media and might be of particular importance in the momentous field of glycomics, where large numbers of protein isoforms need to be characterized. •Simple handling, small volume, easy electrode replacement and automated measurement.•Quick setup with all materials and accessories commercially available.•Convenient for characterization of other redox-active biomolecules.
ISSN:1388-2481
1873-1902
DOI:10.1016/j.elecom.2017.09.016