ANALYTICAL METHOD OPTIMIZATION AND VALIDATION OF GLIBENCLAMIDE AND METFORMIN HYDROCHLORIDE IN DIABETIC HERBS PRODUCT BY THIN-LAYER CHROMATOGRAPHY-DENSITOMETRY

Objective: This study aimed to develop and validate an analytical method using thin-layer chromatography (TLC)-densitometry for detecting thepresence of glibenclamide and metformin hydrochloride in herbal diabetic products.Methods: The jamu samples were extracted using methanol, followed by analysis...

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Vydané v:International journal of applied pharmaceutics s. 83 - 87
Hlavní autori: MAGGADANI, BAITHA PALANGGATAN, SUGIANTO, NOVIANI, HAYUN
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: 21.03.2020
ISSN:0975-7058, 0975-7058
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Abstract Objective: This study aimed to develop and validate an analytical method using thin-layer chromatography (TLC)-densitometry for detecting thepresence of glibenclamide and metformin hydrochloride in herbal diabetic products.Methods: The jamu samples were extracted using methanol, followed by analysis using TLC-densitometry with methanol-aquades-glacial acetic acid(9:1:0.25) as the mobile phase at a wavelength of 237 nm.Results: From the validation results, the correlation coefficients for glibenclamide and metformin hydrochloride were determined to be 0.9998and 0.9981, respectively. The limits of detection and quantitation for glibenclamide were 49.97 μg/mL and 66.55 μg/mL, respectively, while thoseof metformin hydrochloride were 74.75 μg/mL and 249.25 μg/mL, respectively. The method developed in this study met the criteria of selectivity,accuracy, and precision. From seven jamu samples tested, four tested positive for glibenclamide, with sample 1=4.9522%, sample 2=4.1495%, sample3=4.2578%, and sample 4=4.9412%.Conclusion: None of the jamu samples contained metformin hydrochloride.
AbstractList Objective: This study aimed to develop and validate an analytical method using thin-layer chromatography (TLC)-densitometry for detecting thepresence of glibenclamide and metformin hydrochloride in herbal diabetic products.Methods: The jamu samples were extracted using methanol, followed by analysis using TLC-densitometry with methanol-aquades-glacial acetic acid(9:1:0.25) as the mobile phase at a wavelength of 237 nm.Results: From the validation results, the correlation coefficients for glibenclamide and metformin hydrochloride were determined to be 0.9998and 0.9981, respectively. The limits of detection and quantitation for glibenclamide were 49.97 μg/mL and 66.55 μg/mL, respectively, while thoseof metformin hydrochloride were 74.75 μg/mL and 249.25 μg/mL, respectively. The method developed in this study met the criteria of selectivity,accuracy, and precision. From seven jamu samples tested, four tested positive for glibenclamide, with sample 1=4.9522%, sample 2=4.1495%, sample3=4.2578%, and sample 4=4.9412%.Conclusion: None of the jamu samples contained metformin hydrochloride.
Author HAYUN
MAGGADANI, BAITHA PALANGGATAN
SUGIANTO, NOVIANI
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