Evaluation of six molecular assays for the detection of Aigai virus

Introduction Aigai virus (AIGV) is the prototype strain of the novel Orthonairovirus parahaemorrhagiae species ( Nairoviridae family), which contains the strains of the previous CCHFV genogroup VI (or Greece/Europe-2 or AP- 92-like). The reclassification was done due to the genetic distance of AIGV...

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Veröffentlicht in:Population medicine Jg. 5; H. October; S. 1 - 6
Hauptverfasser: Pappa, Styliani, Papa, Anna
Format: Journal Article
Sprache:Englisch
Veröffentlicht: European Publishing 27.10.2023
Schlagworte:
aigai virus
molecular assays
orthonairovirus
ISSN:2654-1459, 2654-1459
Online-Zugang:Volltext
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Zusammenfassung:Introduction Aigai virus (AIGV) is the prototype strain of the novel Orthonairovirus parahaemorrhagiae species ( Nairoviridae family), which contains the strains of the previous CCHFV genogroup VI (or Greece/Europe-2 or AP- 92-like). The reclassification was done due to the genetic distance of AIGV from all genotypes of CCHFV. The aim of the present study was to evaluate the performance of six molecular assays to detect AIGV. Methods Undiluted and serial dilutions (1:10 to 1:10000) of culture supernatant of AIGV strain Pentalofos were used for the comparative study. The strain was isolated from Rhipicephalus bursa ticks removed in 2015 from a goat in Pentalofos village, Greece. Following RNA extraction, six different molecular assays were applied: two nested RT-PCRs, one RT-PCR, and three real-Time RT-PCRs (one commercial). Results All assays detected AIGV up to the 1:1000 dilution, while even higher sensitivity (detection of the 1:10000 dilution) was seen in the nested PCRs designed/modified based on the AP92 sequence, and in two of the real-time RT-PCRs. Lowest Ct values were taken using the commercial assay. Conclusions All assays performed well for the detection of AIGV, suggesting that the risk for underdiagnosis of AIGV infections is low using these assays. However, mismatches in the primers/probes affected the sensitivity of the assays. Genetic surveillance is needed to monitor the mutations in the virus which might affect the efficacy of the diagnostic tools, while a sensitive real time RT-PCR capable to differentiate AIGV and CCHFV will be extremely helpful to estimate the exact burden of AIGV infections.
ISSN:2654-1459
2654-1459
DOI:10.18332/popmed/172259