3058 – METABOLIC HETEROGENEITY IN HEMATOPOIETIC STEM AND PROGENITOR CELLS FUELS INNATE IMMUNITY
Recent technical advances permit metabolic profiling at the single-cell level but existing methods are destructive, making it difficult to link metabolic state to functional outcomes in vivo. Here we perform simultaneous enzyme/ transporter expression and lineage tracing analysis (MetaFate) in singl...
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| Published in: | Experimental hematology Vol. 111; pp. S73 - S74 |
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| Main Authors: | , |
| Format: | Journal Article |
| Language: | English |
| Published: |
Elsevier Inc
2022
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| Subjects: | |
| ISSN: | 0301-472X, 1873-2399 |
| Online Access: | Get full text |
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| Summary: | Recent technical advances permit metabolic profiling at the single-cell level but existing methods are destructive, making it difficult to link metabolic state to functional outcomes in vivo. Here we perform simultaneous enzyme/ transporter expression and lineage tracing analysis (MetaFate) in single Hematopoietic Stem and Progenitor Cells (HSPCs) in mice, identifying metabolic heterogeneity that confers differences in lineage differentiation potential. Combined with single-cell metabolic profiling and functional assays, we characterise a myeloid-biased progenitor compartment with distinct metabolic properties that expands to fuel emergency myelopoiesis following acute infection. Together, our data shows that the metabolic reprogramming of stem and progenitor cells in the bone marrow is necessary to fuel innate immune cell production |
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| ISSN: | 0301-472X 1873-2399 |
| DOI: | 10.1016/j.exphem.2022.07.114 |