A pilot study of red complex and three genera subgingival microbiome in periodontitis subjects with and without diabetes, evaluated by MinION platform [version 2; peer review: 1 approved, 1 approved with reservations]
Background: Subgingival niche is one biofilm habitat containing rich microbiota, which plays an active role in maintaining the health of periodontal tissue and determining host response. As such, a study of changing subgingival biofilms is important for understanding the effect of a systemic conditi...
Saved in:
| Published in: | F1000 research Vol. 10; p. 79 |
|---|---|
| Main Authors: | , , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
2021
|
| Subjects: | |
| ISSN: | 2046-1402, 2046-1402 |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Abstract | Background: Subgingival niche is one biofilm habitat containing rich microbiota, which plays an active role in maintaining the health of periodontal tissue and determining host response. As such, a study of changing subgingival biofilms is important for understanding the effect of a systemic condition. In this study, we compared the occurrence of six bacteria cohabiting in the subgingival area of periodontitis subjects, with (DP, n = 8) and without (NDP, n = 4) diabetes.
Methods: The six genus and species of targeted bacteria were confirmed by 16S rRNA amplicon sequencing on MinION nanopore platform. Descriptive statistic was used to describe the obtained data.
Results: We found that the six genus and species of targeted bacteria were detected but in different quantities in either group's periodontal pocket. Our data showed that Tannerella forsythia was the most abundant species in subgingival biofilms of the DP group of the red complex bacteria. In contrast, Aggregatibacter sp., which belongs to the phylum of proteobacteria, was present at a relatively lower level. In contrast, Fusobacterium sp., which belongs to orange complex bacteria, showed relative similarities in subgingival biofilms of both groups tested, while Veillonella sp., were abundant in the DP groups.
Conclusions: Our data show that the diversity of classic periodontopathogens increased in the subgingival niche of periodontitis subjects with diabetes. It is the first study in Indonesia to apply MinION-based, full-length 16S rRNA amplicon sequencing in periodontitis patients with and without diabetes. |
|---|---|
| AbstractList | Background: Subgingival niche is one biofilm habitat containing rich microbiota, which plays an active role in maintaining the health of periodontal tissue and determining host response. As such, a study of changing subgingival biofilms is important for understanding the effect of a systemic condition. In this study, we compared the occurrence of six bacteria cohabiting in the subgingival area of periodontitis subjects, with (DP, n = 8) and without (NDP, n = 4) diabetes.
Methods: The six genus and species of targeted bacteria were confirmed by 16S rRNA amplicon sequencing on MinION nanopore platform. Descriptive statistic was used to describe the obtained data.
Results: We found that the six genus and species of targeted bacteria were detected but in different quantities in either group's periodontal pocket. Our data showed that Tannerella forsythia was the most abundant species in subgingival biofilms of the DP group of the red complex bacteria. In contrast, Aggregatibacter sp., which belongs to the phylum of proteobacteria, was present at a relatively lower level. In contrast, Fusobacterium sp., which belongs to orange complex bacteria, showed relative similarities in subgingival biofilms of both groups tested, while Veillonella sp., were abundant in the DP groups.
Conclusions: Our data show that the diversity of classic periodontopathogens increased in the subgingival niche of periodontitis subjects with diabetes. It is the first study in Indonesia to apply MinION-based, full-length 16S rRNA amplicon sequencing in periodontitis patients with and without diabetes. Background : Subgingival niche is one biofilm habitat containing rich microbiota, which plays an active role in maintaining the health of periodontal tissue and determining host response. As such, a study of changing subgingival biofilms is important for understanding the effect of a systemic condition. In this study, we compared the occurrence of six bacteria cohabiting in the subgingival area of periodontitis subjects, with (DP, n = 8) and without (NDP, n = 4) diabetes. Methods : The six genus and species of targeted bacteria were confirmed by 16S rRNA amplicon sequencing on MinION nanopore platform. Descriptive statistic was used to describe the obtained data. Results : We found that the six genus and species of targeted bacteria were detected but in different quantities in either group's periodontal pocket. Our data showed that Tannerella forsythia was the most abundant species in subgingival biofilms of the DP group of the red complex bacteria. In contrast, Aggregatibacter sp., which belongs to the phylum of proteobacteria, was present at a relatively lower level. In contrast, Fusobacterium sp., which belongs to orange complex bacteria, showed relative similarities in subgingival biofilms of both groups tested, while Veillonella sp., were abundant in the DP groups. Conclusions: Our data show that the diversity of classic periodontopathogens increased in the subgingival niche of periodontitis subjects with diabetes. It is the first study in Indonesia to apply MinION-based, full-length 16S rRNA amplicon sequencing in periodontitis patients with and without diabetes. Background: Subgingival niche is one biofilm habitat containing rich microbiota, which plays an active role in maintaining the health of periodontal tissue and determining host response. As such, a study of changing subgingival biofilms is important for understanding the effect of a systemic condition. In this study, we compared the occurrence of six bacteria cohabiting in the subgingival area of periodontitis subjects, with (DP, n = 8) and without (NDP, n = 4) diabetes. Methods: The six genus and species of targeted bacteria were confirmed by 16S rRNA amplicon sequencing on MinION nanopore platform. Descriptive statistic was used to describe the obtained data. Results: We found that the six genus and species of targeted bacteria were detected but in different quantities in either group's periodontal pocket. Our data showed that Tannerella forsythia was the most abundant species in subgingival biofilms of the DP group of the red complex bacteria. In contrast, Aggregatibacter sp., which belongs to the phylum of proteobacteria, was present at a relatively lower level. In contrast, Fusobacterium sp., which belongs to orange complex bacteria, showed relative similarities in subgingival biofilms of both groups tested, while Veillonella sp., were abundant in the DP groups. Conclusions: Our data show that the diversity of classic periodontopathogens increased in the subgingival niche of periodontitis subjects with diabetes. It is the first study in Indonesia to apply MinION-based, full-length 16S rRNA amplicon sequencing in periodontitis patients with and without diabetes.Background: Subgingival niche is one biofilm habitat containing rich microbiota, which plays an active role in maintaining the health of periodontal tissue and determining host response. As such, a study of changing subgingival biofilms is important for understanding the effect of a systemic condition. In this study, we compared the occurrence of six bacteria cohabiting in the subgingival area of periodontitis subjects, with (DP, n = 8) and without (NDP, n = 4) diabetes. Methods: The six genus and species of targeted bacteria were confirmed by 16S rRNA amplicon sequencing on MinION nanopore platform. Descriptive statistic was used to describe the obtained data. Results: We found that the six genus and species of targeted bacteria were detected but in different quantities in either group's periodontal pocket. Our data showed that Tannerella forsythia was the most abundant species in subgingival biofilms of the DP group of the red complex bacteria. In contrast, Aggregatibacter sp., which belongs to the phylum of proteobacteria, was present at a relatively lower level. In contrast, Fusobacterium sp., which belongs to orange complex bacteria, showed relative similarities in subgingival biofilms of both groups tested, while Veillonella sp., were abundant in the DP groups. Conclusions: Our data show that the diversity of classic periodontopathogens increased in the subgingival niche of periodontitis subjects with diabetes. It is the first study in Indonesia to apply MinION-based, full-length 16S rRNA amplicon sequencing in periodontitis patients with and without diabetes. |
| Author | Bachtiar, Endang W Theodorea, Citra F Bachtiar, Boy M Natalina Astrella, Cindy Tahapary, Dicky L |
| Author_xml | – sequence: 1 givenname: Boy M orcidid: 0000-0002-5286-2804 surname: Bachtiar fullname: Bachtiar, Boy M organization: Department of Oral Biology and Oral Science Research Center, Faculty of Dentistry, Universitas Indonesia, Jakarta, 10430, Indonesia – sequence: 2 givenname: Citra F surname: Theodorea fullname: Theodorea, Citra F organization: Department of Oral Biology and Oral Science Research Center, Faculty of Dentistry, Universitas Indonesia, Jakarta, 10430, Indonesia – sequence: 3 givenname: Dicky L surname: Tahapary fullname: Tahapary, Dicky L organization: Metabolic, Cardiovascular and Aging Cluster, The Indonesian Medical Education and Research Institute, Faculty of Medicine, Universitas Indonesia, Jakarta, 10430, Indonesia – sequence: 4 givenname: Cindy surname: Astrella fullname: Astrella, Cindy organization: Department of Internal Medicine, Dr. Cipto Mangunkusumo National Referral Hospital, Faculty of Medicine, Universitas Indonesia, Jakarta, 10430, Indonesia – sequence: 5 givenname: Natalina orcidid: 0000-0003-0472-4009 surname: - fullname: -, Natalina organization: Department of Periodontology, Faculty of Dentistry, Universitas Indonesia, Jakarta, 10430, Indonesia – sequence: 6 givenname: Endang W surname: Bachtiar fullname: Bachtiar, Endang W email: endang04@ui.ac.id organization: Department of Oral Biology and Oral Science Research Center, Faculty of Dentistry, Universitas Indonesia, Jakarta, 10430, Indonesia |
| BookMark | eNqFkUFv1DAQhSNUJErpX0Bz5NBdbGdjJ-VUVQUqlfZSTghFjj3edZXYwXZS9qfyb_DuItSeepp3eN-b0by3xZHzDoviPSVLynhdfzSUEBIwogxqs2Q1o3zJXhXHjKz4gq4IO3qi3xSnMT5kgDRNyZk4Lv5cwGh7nyCmSW_BGwioQflh7PE3SKchbQIirNFhkBCnbm3d2s6yh8Gq4DvrBwTrYMRgvfYu2WTjzveAKkV4tGmzj9kJPyXQVnaYMJ4B5pBJpryu28I3667vbmHsZTI-DPBjxhCtd8A-5WgM-azZ4uM5UJDjGPyM-uyJPuzZvSHMMmUu_nxXvDayj3j6b54U3z9f3V9-Xdzcfbm-vLhZKCoatugalJUSWpas4aWpUNc1EcasOlaXRmPZSIWK8K4RRNNO6EqIznDGm0o3xIjypPhwyM2X_JowpnawUWHfS4d-im1JayYIF1WZrfxgzY-LMaBpx2AHGbYtJe2-zvZZne2-zpZl8PwAGqmmPm13pva_6wX4L3qAr6s |
| Cites_doi | 10.1128/JCM.43.8.3944-3955.2005 10.1016/j.chom.2011.10.008 10.1128/IAI.00733-06 10.1111/j.1600-051x.1998.tb02419.x 10.1111/jicd.12051 10.1093/gigascience/giy140 10.1371/journal.pone.0061516 10.1100/2012/278352 10.1038/ismej.2012.174 10.3389/fmicb.2019.01716 10.1099/mic.0.26082-0 10.1128/jcm.40.3.821-825.2002 10.1128/JCM.00053-18 10.1111/j.1600-0757.2005.00113.x 10.1177/154405910308200503 10.1590/1678-77572016-0302 10.1111/jcpe.12059 10.1111/j.1600-0757.2005.00107.x 10.1111/j.1600-0765.1995.tb01284.x 10.1128/MMBR.00024-07 10.3402/jom.v2i0.5818 10.1186/s12864-016-3254-5 10.1111/j.1600-051X.2010.01679.x 10.1128/JCM.00317-06 10.1016/j.micpath.2019.01.009 10.1111/j.1600-051x.1995.tb00176.x 10.3389/fmicb.2016.00840 10.1371/journal.pone.0182992 10.1007/s11262-019-01658-y 10.1007/s00125-011-2342-y 10.1177/00220345990780100801 10.1111/j.1600-0765.2011.01455.x 10.1155/2013/587279 10.1093/gigascience/giy169 10.1080/20002297.2020.1723975 10.2337/dc14-3008 10.3402/jom.v3i0.8396 10.1186/s13742-016-0111-z 10.1111/j.1600-0757.1994.tb00020.x 10.1902/annals.1996.1.1.37 10.1016/j.anaerobe.2013.06.010 10.1111/j.2041-1014.2012.00663.x 10.1093/clinchem/36.6.1258b 10.1111/j.1399-302X.2007.00341.x 10.1111/j.1600-0757.1997.tb00190.x 10.1111/j.1600-051X.2006.00996.x 10.1111/j.1601-0825.2008.01442.x 10.1002/JPER.18-0157 10.3389/fcimb.2014.00092 10.1902/jop.2000.71.11.1699 10.1034/j.1600-051x.2003.00292.x 10.1038/srep33735 10.1007/s00784-011-0654-4 10.1128/JB.02512-14 10.1111/j.1600-051X.2009.01447.x 10.1034/j.1600-051x.2000.027012897.x 10.1186/gb-2012-13-6-r42 10.1159/000329667 10.1016/j.bdq.2015.02.001 |
| ContentType | Journal Article |
| Copyright | Copyright: © 2021 Bachtiar BM et al. |
| Copyright_xml | – notice: Copyright: © 2021 Bachtiar BM et al. |
| DBID | C-E CH4 AAYXX CITATION 7X8 |
| DOI | 10.12688/f1000research.28216.2 |
| DatabaseName | F1000Research Faculty of 1000 CrossRef MEDLINE - Academic |
| DatabaseTitle | CrossRef MEDLINE - Academic |
| DatabaseTitleList | CrossRef MEDLINE - Academic |
| Database_xml | – sequence: 1 dbid: 7X8 name: MEDLINE - Academic url: https://search.proquest.com/medline sourceTypes: Aggregation Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Medicine Women's Studies |
| EISSN | 2046-1402 |
| ExternalDocumentID | 10_12688_f1000research_28216_2 |
| GrantInformation_xml | – fundername: Ministry of Research and Technology/BRIN grantid: UN2.RST/HKP.05.00/2020 |
| GroupedDBID | 3V. 53G 5VS 7X7 88I 8FE 8FH 8FI 8FJ ABUWG ACGOD ACPRK ADACO ADBBV ADRAZ AFKRA AHMBA ALMA_UNASSIGNED_HOLDINGS AZQEC BAWUL BBAFP BBNVY BCNDV BENPR BHPHI BPHCQ BVXVI C-E CH4 DIK DWQXO FRP FYUFA GNUQQ GROUPED_DOAJ GX1 HCIFZ HYE KQ8 LK8 M2P M48 M7P OK1 PIMPY PQEST PQQKQ PQUKI PRINS PROAC RPM AAFWJ AAYXX AFFHD AFPKN AOIJS CCPQU CITATION HMCUK M~E PGMZT PHGZM PHGZT PQGLB UKHRP W2D 7X8 PUEGO |
| ID | FETCH-LOGICAL-c1792-b9ea5c7da32963f5ed8807ff4b283fde39acec06b970d1b7d577bf62695d90f73 |
| ISSN | 2046-1402 |
| IngestDate | Fri Sep 05 17:49:32 EDT 2025 Sat Nov 29 06:39:45 EST 2025 Tue Jun 22 01:10:55 EDT 2021 |
| IsDoiOpenAccess | true |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Keywords | 16S rRNA MinION Subgingival Microbiome Diabetes Periodontitis Red Complex bacteria |
| Language | English |
| License | http://creativecommons.org/licenses/by/4.0/: This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| LinkModel | OpenURL |
| MergedId | FETCHMERGED-LOGICAL-c1792-b9ea5c7da32963f5ed8807ff4b283fde39acec06b970d1b7d577bf62695d90f73 |
| Notes | new_version ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| ORCID | 0000-0002-5286-2804 0000-0003-0472-4009 |
| OpenAccessLink | http://dx.doi.org/10.12688/f1000research.28216.2 |
| PQID | 3182706753 |
| PQPubID | 23479 |
| ParticipantIDs | proquest_miscellaneous_3182706753 crossref_primary_10_12688_f1000research_28216_2 faculty1000_research_10_12688_f1000research_28216_2 |
| ProviderPackageCode | C-E CH4 |
| PublicationCentury | 2000 |
| PublicationDate | 2021 2021-00-00 20210101 |
| PublicationDateYYYYMMDD | 2021-01-01 |
| PublicationDate_xml | – year: 2021 text: 2021 |
| PublicationDecade | 2020 |
| PublicationTitle | F1000 research |
| PublicationYear | 2021 |
| References | M Cullinan (ref-51) 2003; 30 K Kusonmano (ref-13) 2012; 2012 E Tenorio (ref-56) 2011 G Hajishengallis (ref-31) 2012; 27 J Hintao (ref-19) 2007; 22 E Böttger (ref-1) 1990; 36 E Chaves (ref-42) 2000; 27 C Castrillon (ref-33) 2015; 6 D Belstrøm (ref-28) 2020; 12 M Zhou (ref-2) 2013; 8 C Repeke (ref-58) 2009; 36 N Suzuki (ref-26) 2013; 2013 E Leys (ref-45) 2002; 40 H Kuramitsu (ref-36) 2007; 71 V Zijnge (ref-23) 2012; 15 T Kigure (ref-29) 1995; 30 S Anand (ref-14) 2016; 6 E Bachtiar (ref-60) 2021 K Honda (ref-32) 2011; 10 N Segata (ref-30) 2012; 13 S Holt (ref-9) 2005; 38 H Thorstensson (ref-38) 1995; 22 B Rosier (ref-43) 2014; 4 V Grossmann (ref-25) 2015; 38 J Caton (ref-12) 2018; 89 Suppl 1 G Armitage (ref-48) 1996; 1 P Kumar (ref-39) 2006; 44 F Carrouel (ref-34) 2016; 7 J He (ref-50) 2012; 16 J Taylor (ref-7) 2013; 40 Suppl 14 L Abusleme (ref-40) 2013; 7 E Kononen (ref-46) 1999; 78 D Belstrøm (ref-17) 2017; 12 W Wade (ref-27) 2011; 38 Suppl 11 T Miranda (ref-53) 2017; 25 G Taylor (ref-3) 2008; 14 B Michalowicz (ref-57) 2000; 71 A Saeb (ref-20) 2019; 128 T Thurnheer (ref-54) 2019; 10 P Preshaw (ref-41) 2012; 55 I Mashima (ref-55) 2015; 197 Y Zhang (ref-11) 2019; 55 P Aemaimanan (ref-5) 2013; 22 L Kesavalu (ref-37) 2007; 75 P Kumar (ref-44) 2003; 82 M Murra (ref-49) 2018; 56 C Field (ref-52) 2012; 47 A Haffajee (ref-47) 1994; 5 P Kumar (ref-22) 2005; 43 E Lalla (ref-18) 2006; 33 T Laver (ref-24) 2015; 3 D Ai (ref-6) 2017; 18 P Marsh (ref-10) 2003; 149 R Darveau (ref-35) 1997; 14 A Benítez-Páez (ref-21) 2016; 5 V Shabardina (ref-15) 2019; 8 S Socransky (ref-8) 1998; 25 S Socransky (ref-59) 2005; 38 S Calus (ref-16) 2018; 7 E Ohlrich (ref-4) 2010 |
| References_xml | – volume: 43 start-page: 3944-55 year: 2005 ident: ref-22 article-title: Identification of candidate periodontal pathogens and beneficial species by quantitative 16S clonal analysis. publication-title: J Clin Microbiol. doi: 10.1128/JCM.43.8.3944-3955.2005 – volume: 10 start-page: 423-5 year: 2011 ident: ref-32 article-title: Porphyromonas gingivalis sinks teeth into the oral microbiota and periodontal disease. publication-title: Cell Host Microbe. doi: 10.1016/j.chom.2011.10.008 – volume: 75 start-page: 1704-12 year: 2007 ident: ref-37 article-title: Rat model of polymicrobial infection, immunity, and alveolar bone resorption in periodontal disease. publication-title: Infect Immun. doi: 10.1128/IAI.00733-06 – volume: 25 start-page: 134-44 year: 1998 ident: ref-8 article-title: Microbial complexes in subgingival plaque. publication-title: J Clin Periodontol. doi: 10.1111/j.1600-051x.1998.tb02419.x – volume: 6 start-page: 25-31 year: 2015 ident: ref-33 article-title: Occurrence of red complex microorganisms and Aggregatibacter actinomycetemcomitans in patients with diabetes. publication-title: J Investig Clin Dent. doi: 10.1111/jicd.12051 – volume: 7 start-page: giy140 year: 2018 ident: ref-16 article-title: NanoAmpli-Seq: a workflow for amplicon sequencing for mixed microbial communities on the nanopore sequencing platform. publication-title: Gigascience. doi: 10.1093/gigascience/giy140 – volume: 8 start-page: e61516 year: 2013 ident: ref-2 article-title: Investigation of the effect of type 2 diabetes mellitus on subgingival plaque microbiota by high-throughput 16S rDNA pyrosequencing. publication-title: PLoS One. doi: 10.1371/journal.pone.0061516 – volume: 2012 start-page: 278352 year: 2012 ident: ref-13 article-title: Effects of Pooling Samples on the Performance of Classification Algorithms: A Comparative Study. publication-title: ScientificWorldJournal. doi: 10.1100/2012/278352 – volume: 7 start-page: 1016-25 year: 2013 ident: ref-40 article-title: The subgingival microbiome in health and periodontitis and its relationship with community biomass and inflammation. publication-title: ISME J. doi: 10.1038/ismej.2012.174 – volume: 10 start-page: 1716 year: 2019 ident: ref-54 article-title: Fusobacterium Species and Subspecies Differentially Affect the Composition and Architecture of Supra- and Subgingival Biofilms Models. publication-title: Front Microbiol. doi: 10.3389/fmicb.2019.01716 – volume: 149 start-page: 279-294 year: 2003 ident: ref-10 article-title: Are dental diseases examples of ecological catastrophes? publication-title: Microbiology (Reading). doi: 10.1099/mic.0.26082-0 – volume: 40 start-page: 821-5 year: 2002 ident: ref-45 article-title: Association of Bacteroides forsythus and a novel Bacteroides phylotype with periodontitis. publication-title: J Clin Microbiol. doi: 10.1128/jcm.40.3.821-825.2002 – volume: 56 year: 2018 ident: ref-49 article-title: Whole-Genome Sequencing of Aggregatibacter Species Isolated from Human Clinical Specimens and Description of Aggregatibacter kilianii sp. nov. publication-title: J Clin Microbiol. doi: 10.1128/JCM.00053-18 – volume: 38 start-page: 72-122 year: 2005 ident: ref-9 article-title: Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia: the "red complex", a prototype polybacterial pathogenic consortium in periodontitis. publication-title: Periodontol 2000. doi: 10.1111/j.1600-0757.2005.00113.x – volume: 82 start-page: 338-44 year: 2003 ident: ref-44 article-title: New bacterial species associated with chronic periodontitis. publication-title: J Dent Res. doi: 10.1177/154405910308200503 – volume: 25 start-page: 82-89 year: 2017 ident: ref-53 article-title: Influence of glycemic control on the levels of subgingival periodontal pathogens in patients with generalized chronic periodontitis and type 2 diabetes. publication-title: J Appl Oral Sci. doi: 10.1590/1678-77572016-0302 – volume: 40 Suppl 14 start-page: S113-34 year: 2013 ident: ref-7 article-title: A review of the evidence for pathogenic mechanisms that may link periodontitis and diabetes. publication-title: J Clin Periodontol. doi: 10.1111/jcpe.12059 – volume: 38 start-page: 135-87 year: 2005 ident: ref-59 article-title: Periodontal microbial ecology. publication-title: Periodontol 2000. doi: 10.1111/j.1600-0757.2005.00107.x – volume: 30 start-page: 332-41 year: 1995 ident: ref-29 article-title: Distribution of Porphyromonas gingivalis and Treponema denticola in human subgingival plaque at different periodontal pocket depths examined by immunohistochemical methods. publication-title: J Periodontal Res. doi: 10.1111/j.1600-0765.1995.tb01284.x – volume: 71 start-page: 653-70 year: 2007 ident: ref-36 article-title: Interspecies interactions within oral microbial communities. publication-title: Microbiol Mol Biol Rev. doi: 10.1128/MMBR.00024-07 – start-page: 2 year: 2010 ident: ref-4 article-title: Diabetes, periodontitis, and the subgingival microbiota. publication-title: J Oral Microbiol. doi: 10.3402/jom.v2i0.5818 – volume: 18 start-page: 1041 year: 2017 ident: ref-6 article-title: Integrated metagenomic data analysis demonstrates that a loss of diversity in oral microbiota is associated with periodontitis. publication-title: BMC Genomics. doi: 10.1186/s12864-016-3254-5 – volume: 38 Suppl 11 start-page: 7-16 year: 2011 ident: ref-27 article-title: Has the use of molecular methods for the characterization of the human oral microbiome changed our understanding of the role of bacteria in the pathogenesis of periodontal disease? publication-title: J Clin Periodontol. doi: 10.1111/j.1600-051X.2010.01679.x – volume: 44 start-page: 3665-73 year: 2006 ident: ref-39 article-title: Changes in periodontal health status are associated with bacterial community shifts as assessed by quantitative 16S cloning and sequencing. publication-title: J Clin Microbiol. doi: 10.1128/JCM.00317-06 – volume: 128 start-page: 215-229 year: 2019 ident: ref-20 article-title: Relative reduction of biological and phylogenetic diversity of the oral microbiota of diabetes and pre-diabetes patients. publication-title: Microb Pathog. doi: 10.1016/j.micpath.2019.01.009 – volume: 22 start-page: 449-58 year: 1995 ident: ref-38 article-title: Some suspected periodontopathogens and serum antibody response in adult long-duration insulin-dependent diabetics. publication-title: J Clin Periodontol. doi: 10.1111/j.1600-051x.1995.tb00176.x – volume: 7 start-page: 840 year: 2016 ident: ref-34 article-title: Quantitative Molecular Detection of 19 Major Pathogens in the Interdental Biofilm of Periodontally Healthy Young Adults. publication-title: Front Microbiol. doi: 10.3389/fmicb.2016.00840 – volume: 12 start-page: e0182992 year: 2017 ident: ref-17 article-title: Microbial profile comparisons of saliva, pooled and site-specific subgingival samples in periodontitis patients. publication-title: PLoS One. doi: 10.1371/journal.pone.0182992 – volume: 55 start-page: 381-393 year: 2019 ident: ref-11 article-title: A novel phage from periodontal pockets associated with chronic periodontitis. publication-title: Virus Genes. doi: 10.1007/s11262-019-01658-y – volume: 55 start-page: 21-31 year: 2012 ident: ref-41 article-title: Periodontitis and diabetes: a two-way relationship. publication-title: Diabetologia. doi: 10.1007/s00125-011-2342-y – volume: 78 start-page: 1634-9 year: 1999 ident: ref-46 article-title: Establishment of oral anaerobes during the first year of life. publication-title: J Dent Res. doi: 10.1177/00220345990780100801 – volume: 47 start-page: 470-8 year: 2012 ident: ref-52 article-title: Investigation and quantification of key periodontal pathogens in patients with type 2 diabetes. publication-title: J Periodontal Res. doi: 10.1111/j.1600-0765.2011.01455.x – volume: 2013 start-page: 587279 year: 2013 ident: ref-26 article-title: Mixed red-complex bacterial infection in periodontitis. publication-title: Int J Dent. doi: 10.1155/2013/587279 – volume: 8 start-page: giy169 year: 2019 ident: ref-15 article-title: NanoPipe-a web server for nanopore MinION sequencing data analysis. publication-title: Gigascience. doi: 10.1093/gigascience/giy169 – volume: 12 start-page: 1723975 year: 2020 ident: ref-28 article-title: The salivary microbiota in health and disease. publication-title: J Oral Microbiol. doi: 10.1080/20002297.2020.1723975 – volume: 38 start-page: 1356-64 year: 2015 ident: ref-25 article-title: Profile of the Immune and Inflammatory Response in Individuals With Prediabetes and Type 2 Diabetes. publication-title: Diabetes Care. doi: 10.2337/dc14-3008 – start-page: 3 year: 2011 ident: ref-56 article-title: Identification of interspecies interactions affecting Porphyromonas gingivalis virulence phenotypes. publication-title: J Oral Microbiol. doi: 10.3402/jom.v3i0.8396 – volume: 5 start-page: 4 year: 2016 ident: ref-21 article-title: Species-level resolution of 16S rRNA gene amplicons sequenced through the MinION™ portable nanopore sequencer. publication-title: Gigascience. doi: 10.1186/s13742-016-0111-z – volume: 5 start-page: 78-111 year: 1994 ident: ref-47 article-title: Microbial etiological agents of destructive periodontal diseases. publication-title: Periodontol 2000. doi: 10.1111/j.1600-0757.1994.tb00020.x – volume: 1 start-page: 37-215 year: 1996 ident: ref-48 article-title: Periodontal diseases: diagnosis. publication-title: Ann Periodontol. doi: 10.1902/annals.1996.1.1.37 – volume: 22 start-page: 64-8 year: 2013 ident: ref-5 article-title: Quantification of key periodontal pathogens in insulin-dependent type 2 diabetic and non-diabetic patients with generalized chronic periodontitis. publication-title: Anaerobe. doi: 10.1016/j.anaerobe.2013.06.010 – volume: 27 start-page: 409-19 year: 2012 ident: ref-31 article-title: Beyond the red complex and into more complexity: the polymicrobial synergy and dysbiosis (PSD) model of periodontal disease etiology. publication-title: Mol Oral Microbiol. doi: 10.1111/j.2041-1014.2012.00663.x – volume: 36 start-page: 1258-9 year: 1990 ident: ref-1 article-title: Frequent contamination of Taq polymerase with DNA. publication-title: Clin Chem. doi: 10.1093/clinchem/36.6.1258b – volume: 22 start-page: 175-81 year: 2007 ident: ref-19 article-title: The microbiological profiles of saliva, supragingival and subgingival plaque and dental caries in adults with and without type 2 diabetes mellitus. publication-title: Oral Microbiol Immunol. doi: 10.1111/j.1399-302X.2007.00341.x – volume: 14 start-page: 12-32 year: 1997 ident: ref-35 article-title: The microbial challenge in periodontitis. publication-title: Periodontol 2000. doi: 10.1111/j.1600-0757.1997.tb00190.x – volume: 33 start-page: 855-62 year: 2006 ident: ref-18 article-title: Periodontal infection profiles in type 1 diabetes. publication-title: J Clin Periodontol. doi: 10.1111/j.1600-051X.2006.00996.x – volume: 14 start-page: 191-203 year: 2008 ident: ref-3 article-title: Periodontal disease: associations with diabetes, glycemic control and complications. publication-title: Oral Dis. doi: 10.1111/j.1601-0825.2008.01442.x – volume: 89 Suppl 1 start-page: S1-S8 year: 2018 ident: ref-12 article-title: A new classification scheme for periodontal and peri-implant diseases and conditions - Introduction and key changes from the 1999 classification. publication-title: J Periodontol. doi: 10.1002/JPER.18-0157 – volume: 4 start-page: 92 year: 2014 ident: ref-43 article-title: Historical and contemporary hypotheses on the development of oral diseases: are we there yet? publication-title: Front Cell Infect Microbiol. doi: 10.3389/fcimb.2014.00092 – volume: 71 start-page: 1699-707 year: 2000 ident: ref-57 article-title: Evidence of a substantial genetic basis for risk of adult periodontitis. publication-title: J Periodontol. doi: 10.1902/jop.2000.71.11.1699 – volume: 30 start-page: 532-41 year: 2003 ident: ref-51 article-title: Acquisition and loss of Porphyromonas gingivalis, Actinobacillus actinomycetemcomitans and Prevotella intermedia over a 5-year period: effect of a triclosan/copolymer dentifrice. publication-title: J Clin Periodontol. doi: 10.1034/j.1600-051x.2003.00292.x – volume: 6 start-page: 33735 year: 2016 ident: ref-14 article-title: Next Generation Sequencing of Pooled Samples: Guideline for Variants' Filtering. publication-title: Sci Rep. doi: 10.1038/srep33735 – volume: 16 start-page: 1579-88 year: 2012 ident: ref-50 article-title: Quantitative analysis of microbiota in saliva, supragingival, and subgingival plaque of Chinese adults with chronic periodontitis. publication-title: Clin Oral Investig. doi: 10.1007/s00784-011-0654-4 – volume: 197 start-page: 2104-2111 year: 2015 ident: ref-55 article-title: The interaction between Streptococcus spp. and Veillonella tobetsuensis in the early stages of oral biofilm formation. publication-title: J Bacteriol. doi: 10.1128/JB.02512-14 – volume: 36 start-page: 726-38 year: 2009 ident: ref-58 article-title: Strong and persistent microbial and inflammatory stimuli overcome the genetic predisposition to higher matrix metalloproteinase-1 (MMP-1) expression: a mechanistic explanation for the lack of association of MMP1-1607 single-nucleotide polymorphism genotypes with MMP-1 expression in chronic periodontitis lesions. publication-title: J Clin Periodontol. doi: 10.1111/j.1600-051X.2009.01447.x – volume: 27 start-page: 897-903 year: 2000 ident: ref-42 article-title: Persistent bacterial colonization of Porphyromonas gingivalis, Prevotella intermedia, and Actinobacillus actinomycetemcomitans in periodontitis and its association with alveolar bone loss after 6 months of therapy. publication-title: J Clin Periodontol. doi: 10.1034/j.1600-051x.2000.027012897.x – volume: 13 start-page: R42 year: 2012 ident: ref-30 article-title: Composition of the adult digestive tract bacterial microbiome based on seven mouth surfaces, tonsils, throat and stool samples. publication-title: Genome Biol. doi: 10.1186/gb-2012-13-6-r42 – volume: 15 start-page: 1-16 year: 2012 ident: ref-23 article-title: Subgingival biofilm structure. publication-title: Front Oral Biol. doi: 10.1159/000329667 – volume: 3 start-page: 1-8 year: 2015 ident: ref-24 article-title: Assessing the performance of the Oxford Nanopore Technologies MinION. publication-title: Biomol Detect Quantif. doi: 10.1016/j.bdq.2015.02.001 – year: 2021 ident: ref-60 article-title: A pilot study of red complex and three genera subgingival microbiome in periodontitis subjects with and without diabetes, evaluated by MinION |
| SSID | ssj0000993627 |
| Score | 2.1669161 |
| Snippet | Background: Subgingival niche is one biofilm habitat containing rich microbiota, which plays an active role in maintaining the health of periodontal tissue and... Background : Subgingival niche is one biofilm habitat containing rich microbiota, which plays an active role in maintaining the health of periodontal tissue... |
| SourceID | proquest crossref faculty1000 |
| SourceType | Aggregation Database Index Database Publisher |
| StartPage | 79 |
| Title | A pilot study of red complex and three genera subgingival microbiome in periodontitis subjects with and without diabetes, evaluated by MinION platform [version 2; peer review: 1 approved, 1 approved with reservations] |
| URI | http://dx.doi.org/10.12688/f1000research.28216.2 https://www.proquest.com/docview/3182706753 |
| Volume | 10 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| journalDatabaseRights | – providerCode: PRVAON databaseName: DOAJ Directory of Open Access Journals customDbUrl: eissn: 2046-1402 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0000993627 issn: 2046-1402 databaseCode: DOA dateStart: 20120101 isFulltext: true titleUrlDefault: https://www.doaj.org/ providerName: Directory of Open Access Journals – providerCode: PRVHPJ databaseName: ROAD: Directory of Open Access Scholarly Resources customDbUrl: eissn: 2046-1402 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0000993627 issn: 2046-1402 databaseCode: M~E dateStart: 20120101 isFulltext: true titleUrlDefault: https://road.issn.org providerName: ISSN International Centre – providerCode: PRVPQU databaseName: AUTh Library subscriptions: ProQuest Central customDbUrl: eissn: 2046-1402 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0000993627 issn: 2046-1402 databaseCode: BENPR dateStart: 20120101 isFulltext: true titleUrlDefault: https://www.proquest.com/central providerName: ProQuest – providerCode: PRVPQU databaseName: Biological Science Database customDbUrl: eissn: 2046-1402 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0000993627 issn: 2046-1402 databaseCode: M7P dateStart: 20120101 isFulltext: true titleUrlDefault: http://search.proquest.com/biologicalscijournals providerName: ProQuest – providerCode: PRVPQU databaseName: Health & Medical Collection customDbUrl: eissn: 2046-1402 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0000993627 issn: 2046-1402 databaseCode: 7X7 dateStart: 20120101 isFulltext: true titleUrlDefault: https://search.proquest.com/healthcomplete providerName: ProQuest – providerCode: PRVPQU databaseName: Publicly Available Content Database customDbUrl: eissn: 2046-1402 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0000993627 issn: 2046-1402 databaseCode: PIMPY dateStart: 20120101 isFulltext: true titleUrlDefault: http://search.proquest.com/publiccontent providerName: ProQuest – providerCode: PRVPQU databaseName: Science Database customDbUrl: eissn: 2046-1402 dateEnd: 99991231 omitProxy: false ssIdentifier: ssj0000993627 issn: 2046-1402 databaseCode: M2P dateStart: 20120101 isFulltext: true titleUrlDefault: https://search.proquest.com/sciencejournals providerName: ProQuest |
| link | http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV1bi9NAFB7Wrog-LLq6uF7KERQfuum2SZNJ9KldVhTsUmSFFZGQyUzYaE1Lb7Q_1d_hH_CcmUna4uINfBnSNDlper7M-ebkXBh7KpRIoygNHNn2pdNpJ74jhO87Mks8T7qqLYUu4vqWn52FFxfRYGfne5kLsxjyogiXy2j8X1WN-1DZlDr7F-quhOIO3Eal44hqx_GPFN9tjPPhaGYKxxIXpAhzHTmuljZecqIUtU5Wk6QxnQtqU5QvKIskN1WZvioTWz7Jcc2qa65O6bjPOvCjSoajDQpqLr23pCxbO9yw2n5eUEef8TCZETNuPPN7C-Oda7jUOWCs1MSmzmi_hKlvvjDe1_Unc0XKkrLu42nZFL3sLkovDxq2alHl3e4l6SXOXsYZMVqtvb5UjYDaq2jWfJLP8E-oopvPk0skDzayIE-_rBqVb7xLWTX4zJizCluT1_pLTPp1U-kZ1W11AgdXlNvTf-tKS-IGIaVHZHQL5R00cX3aDpru2naW8QInzun2zmqBRXLiLSmxlhIjb9h1Oa7namx38KY_-FC5BpG0I63gNp-dBBxf-TO2qNStLKFaLSs68CdeocnS-W22Z1c50DXovMN2VLHPbvRtHMc-29ONU59PwYax3mXfuqBhCxq2MMoAYQsWtoB4Aw1bMLCFDdjCGraQF7AFWyhhCwQiLcbCFkrYHkEFWhArMKCFErTw0UIW3JdAgAUD2BfQhhKgRxvb5jqbYP10j71_dXp-8tqxbUecFK2T64hIJX7KZeK5aJ0yX0m0cTzLOgKpeCaVFyWpSluBiHhLtgWXPuciC9wg8mXUyrh3wGrFqFD3GSRcBmkQCk9yhczZF2GgOBLwDIlzFGadQ3ZcajAem-oy8a9Bc8i8DUXH1de_O-tJCYgYzQe9E0wKNZpPYzTpLievgffgnyQ_ZDfpITNOyUesNpvM1WN2PV2gjid1do1fcD2GdbbbOz0bvKtrrxeOfXdAI8fR4P8HYnj77Q |
| linkProvider | ProQuest |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=A+pilot+study+of+red+complex+and+three+genera+subgingival+microbiome+in+periodontitis+subjects+with+and+without+diabetes%2C+evaluated+by+MinION+platform+%5Bversion+2%3B+peer+review%3A+1+approved%2C+1+approved+with+reservations%5D&rft.jtitle=F1000+research&rft.au=Bachtiar%2C+Boy+M&rft.au=Theodorea%2C+Citra+F&rft.au=Tahapary%2C+Dicky+L&rft.au=Astrella%2C+Cindy&rft.date=2021&rft.eissn=2046-1402&rft.volume=10&rft_id=info:doi/10.12688%2Ff1000research.28216.2&rft.externalDBID=C-E&rft.externalDocID=10_12688_f1000research_28216_2 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2046-1402&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2046-1402&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2046-1402&client=summon |