The effect of vitrectomy on changes in molecular biomarker levels of the vitreous cavity in the early postoperative period in proliferative diabetic retinopathy

Determining the levels of molecular biomarkers detected in the vitreous body in various pathological conditions (including diabetic retinopathy), allows for a better understanding of their pathogenesis and optimization of treatment approaches. The multiplex assay is a modern alternative to enzyme-li...

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Vydáno v:Vestnik oftal'mologii Ročník 141; číslo 2; s. 30
Hlavní autoři: Yusef, Yu, Petrachkov, D V, Baryshev, K V, Matyushchenko, A G
Médium: Journal Article
Jazyk:ruština
Vydáno: Russia (Federation) 2025
Témata:
Adult
Aged
Biomarkers - analysis
Biomarkers - metabolism
Diabetic Retinopathy - diagnosis
Diabetic Retinopathy - etiology
Diabetic Retinopathy - metabolism
Diabetic Retinopathy - surgery
Female
Follow-Up Studies
Humans
Male
Middle Aged
Postoperative Period
Treatment Outcome
Vascular Endothelial Growth Factor A - metabolism
Vitrectomy - adverse effects
Vitrectomy - methods
Vitreous Body - metabolism
molecular biomarkers
vitrectomy
diabetic retinopathy
multiplex assay
ISSN:0042-465X
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Shrnutí:Determining the levels of molecular biomarkers detected in the vitreous body in various pathological conditions (including diabetic retinopathy), allows for a better understanding of their pathogenesis and optimization of treatment approaches. The multiplex assay is a modern alternative to enzyme-linked immunosorbent assay (ELISA), enabling the simultaneous analysis of a large number of biomarkers. This study was performed to identify changes in the levels of vitreous biomarkers in patients with proliferative diabetic retinopathy (PDR) in the early postoperative period following 25-gauge vitrectomy. The study included 24 patients (24 eyes) with type 2 diabetes mellitus complicated by PDR requiring surgical intervention for tractional retinal detachment, subtotal or total vitreous hemorrhage, or tractional diabetic macular edema (DME). Vitreous samples were collected from all patients at the first stage of vitrectomy and one month after surgery. One month after surgery, the level of the pro-inflammatory cytokine vascular endothelial growth factor (VEGF) decreased from 203.8±228.0 to 113±95 pg/ml; however, the difference was not statistically significant ( =0.307). A significant reduction was observed in the concentrations of the following pro-inflammatory biomarkers: bFGF, CTACK, Eotaxin, HGF, IL-1β, -1α, -4, -12 (p40), -16, -18, IL-2Rα, IP-10, MIG, SCF, SCGF-β, SDF-1α, MIF, and M-CSF. The main effect of vitrectomy on pro-inflammatory cytokines is reduction of their concentration, which confirms the buffering role of the vitreous body in the accumulation of molecular biomarkers in diabetic retinopathy.
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content type line 23
ISSN:0042-465X
DOI:10.17116/oftalma202514102130