A Hierarchical Coding Strategy for Live Cell Imaging of Protein‐Specific Glycoform

Live cell imaging of protein‐specific glycoforms holds great promise for revolutionizing the study of glycochemistry. The imaging protocols developed thus far build upon the paired interplay of probe units, thus limiting the number of monosaccharide identification channels. A hierarchical coding (Hi...

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Vydáno v:Angewandte Chemie Ročník 130; číslo 37; s. 12183 - 12187
Hlavní autoři: Li, Siqiao, Liu, Yiran, Liu, Lu, Feng, Yimei, Ding, Lin, Ju, Huangxian
Médium: Journal Article
Jazyk:angličtina
Vydáno: Weinheim Wiley Subscription Services, Inc 10.09.2018
Témata:
Bildgebung lebender Zellen
Channels
Chemistry
Decoding
Deoxyribonucleic acid
DNA
Fucose
Glycoproteins
Glykosylierungen
Imaging
Monosaccharides
Proteins
Proteinspezifische Glykoform
Stoffwechsel-Engineering
ISSN:0044-8249, 1521-3757
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Shrnutí:Live cell imaging of protein‐specific glycoforms holds great promise for revolutionizing the study of glycochemistry. The imaging protocols developed thus far build upon the paired interplay of probe units, thus limiting the number of monosaccharide identification channels. A hierarchical coding (HieCo) imaging strategy, with DNA coding and decoding of protein and monosaccharides executed in fidelity to the hierarchical order of target glycoprotein, is reported herein and features expandable monosaccharide identification channels. A proof‐of‐concept protocol has been developed for MUC1‐specific imaging of terminal sialic acid (Sia) and fucose (Fuc) on MCF‐7, T47D, MDA‐MB‐231, and PANC‐1 cells, revealing distinct monosaccharide patterns for four types of cells. The protocol also permits dynamic monitoring of changes in MUC1‐specific monosaccharide patterns associated with both the alteration of cellular physiological states and the occurrence of a biologically important process. Eine hierarchische Kodierungsstrategie für die Bildgebung proteinspezifischer Glykoform in lebenden Zellen wurde entwickelt, bei der DNA jedes Strukturmotiv getreu der hierarchischen Ordnung des gesuchten Glykoproteins kodiert und dekodiert. Die Strategie nutzt expandierbare Monosaccharid‐Identifizierungskanäle, und sie sollte ein vollständigeres Bild der Proteinglykosylierungsmechanismen und ‐funktionen liefern.
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ISSN:0044-8249
1521-3757
DOI:10.1002/ange.201807054