Cracking Controls ATP Hydrolysis in the catalytic unit of a P-type ATPase

[Display omitted] •The isolated AfCopA-NP catalytic unit conserves its optimal working temperature and apparent affinity for ATP.•Small additions of urea increase ATPase activity.•A characterization of the native conformational ensemble was performed by a combination of local frustration and AlphaFo...

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Bibliographic Details

Published in:	Journal of molecular biology p. 169551
Main Authors:	Placenti, M.Agueda, Martinez-Gache, Santiago A., González-Lebrero, Rodolfo M., Wolynes, Peter G., González Flecha, F.Luis, Roman, Ernesto A.
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier Ltd 20.11.2025
Subjects:	ATPase activity Catalytic unit Local frustration Local unfolding PIB-ATPases Catalytic unit Local unfolding PIB-ATPases ATPase activity Local frustration P(IB)-ATPases
ISSN:	0022-2836, 1089-8638, 1089-8638
Online Access:	Get full text
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