The effects of Interleukin-6 on viability, redox homeostasis and migration capacity of human placental cells JEG-3 in chemically induced hypoxia
Interleukin-6 (IL-6) is a pleiotropic cytokine involved in the regulation of cell growth and differentiation exerting an important role in the immune and inflammatory response. This study aimed to evaluate the effects of three increasing concentrations of IL-6 (1 pg/mL, 5 pg/mL, and 10 pg/mL) on cel...
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| Vydané v: | Kragujevac Journal of Science Ročník 2022; číslo 44; s. 103 - 114 |
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| Hlavní autori: | , , , , |
| Médium: | Journal Article |
| Jazyk: | English |
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University of Kragujevac, Faculty of Science
2022
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| ISSN: | 1450-9636, 2466-5509 |
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| Abstract | Interleukin-6 (IL-6) is a pleiotropic cytokine involved in the regulation of cell growth and differentiation exerting an important role in the immune and inflammatory response. This study aimed to evaluate the effects of three increasing concentrations of IL-6 (1 pg/mL, 5 pg/mL, and 10 pg/mL) on cell viability, redox homeostasis parameters (O2∙, NO2-, glutathione) and migratory potential in human trophoblast cell line JEG-3 under chemically induced hypoxia in short-term (24 h) and long-term (72 h) exposure. The obtained results show a dose-dependent reduction of cell viability and NO levels, while the concentration of O2 ∙increased. Levels of total glutathione increased in a dose-dependent manner compared to control cells, suggesting its significant antioxidative contribution in hypoxic conditions. The migratory potential of cells was significantly elevated in the two highest applied doses implying the disturbance of cell invasive homeostasis at its pathological concentrations, which could represent a risk factor in some pregnancy disorders. |
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| AbstractList | Interleukin-6 (IL-6) is a pleiotropic cytokine involved in the regulation of cell growth and differentiation exerting an important role in the immune and inflammatory response. This study aimed to evaluate the effects of three increasing concentrations of IL-6 (1 pg/mL, 5 pg/mL, and 10 pg/mL) on cell viability, redox homeostasis parameters (O2∙, NO2-, glutathione) and migratory potential in human trophoblast cell line JEG-3 under chemically induced hypoxia in short-term (24 h) and long-term (72 h) exposure. The obtained results show a dose-dependent reduction of cell viability and NO levels, while the concentration of O2 ∙increased. Levels of total glutathione increased in a dose-dependent manner compared to control cells, suggesting its significant antioxidative contribution in hypoxic conditions. The migratory potential of cells was significantly elevated in the two highest applied doses implying the disturbance of cell invasive homeostasis at its pathological concentrations, which could represent a risk factor in some pregnancy disorders. |
| Author | Matić, Miloš Obradović, Ana Paunović, Milica Milošević, Marija Ognjanović, Branka |
| Author_xml | – sequence: 1 givenname: Miloš surname: Matić fullname: Matić, Miloš – sequence: 2 givenname: Ana orcidid: 0000-0002-6372-1062 surname: Obradović fullname: Obradović, Ana – sequence: 3 givenname: Marija surname: Milošević fullname: Milošević, Marija – sequence: 4 givenname: Milica surname: Paunović fullname: Paunović, Milica – sequence: 5 givenname: Branka surname: Ognjanović fullname: Ognjanović, Branka |
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| Cites_doi | 10.1002/cber.187901201117 10.1073/pnas.87.1.355 10.1093/humupd/dmi023 10.1016/j.placenta.2012.01.020 10.1038/labinvest.2014.76 10.1159/000087834 10.1016/0022-1759(83)90303-4 10.1016/S0076-6879(85)13073-9 10.1093/acprof:oso/9780198717478.003.0003 10.3390/pathophysiology28020013 10.3892/etm.2016.3128 10.1038/sj.bjp.0702757 10.1073/pnas.90.17.7915 10.1161/CIRCRESAHA.117.311401 10.1158/2326-6066.CIR-14-0022 10.1016/j.cub.2014.03.034 10.1016/j.placenta.2009.01.013 10.1186/1471-2393-5-14 10.1097/WOX.0b013e3182439613 10.1093/molehr/2.4.239 10.1080/19336918.2015.1120397 |
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| Title | The effects of Interleukin-6 on viability, redox homeostasis and migration capacity of human placental cells JEG-3 in chemically induced hypoxia |
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