Bio-orthogonal Metabolic Fluorine Labeling Enables Deep-Tissue Visualization of Tumor Cells In Vivo by 19 F Magnetic Resonance Imaging
The high resolution, deep penetration, and negligible biological background of F magnetic resonance imaging (MRI) makes it a potential means for imaging various biological targets in vivo. However, the limited targeting strategies of current F MRI probes significantly restrict their applications for...
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| Vydáno v: | Analytical chemistry (Washington) Ročník 94; číslo 48; s. 16614 |
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| Hlavní autoři: | , , , , , , |
| Médium: | Journal Article |
| Jazyk: | angličtina |
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United States
06.12.2022
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| Témata: | |
| ISSN: | 1520-6882 |
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| Abstract | The high resolution, deep penetration, and negligible biological background of
F magnetic resonance imaging (MRI) makes it a potential means for imaging various biological targets in vivo. However, the limited targeting strategies of current
F MRI probes significantly restrict their applications for in vivo tracking of low-abundance targets and specific biological processes, which greatly stimulates the investigations on new targeting methods for
F MRI. Herein, we report a strategy, termed as bio-orthogonal metabolic fluorine labeling, for selective cellular
F labeling, which permits in vivo imaging of tumor cells with high specificity. This strategy exploits the display of azido groups on the cell surface via selective uptake and metabolic engineering of tetra-acetylated
-azidoacetylmannosamine (Ac
ManAz) by cancer cells and subsequent rapid and specific bio-orthogonal ligation between azido and cyclootynyl groups to incorporate
F-containing moieties on the surface of cancer cells. We validated the feasibility of this method on the cellular level with A549 and HepG2 cells and further illustrated the application of this method for in vivo deep-tissue visualization of cancer cells with A549 tumor-bearing BALB/c mice using hot spot
F MRI. Our strategy expands the arsenal for targeted
F MRI and provides a promising method for imaging biological targets in living subjects with high tissue penetration and low biological background. |
|---|---|
| AbstractList | The high resolution, deep penetration, and negligible biological background of
F magnetic resonance imaging (MRI) makes it a potential means for imaging various biological targets in vivo. However, the limited targeting strategies of current
F MRI probes significantly restrict their applications for in vivo tracking of low-abundance targets and specific biological processes, which greatly stimulates the investigations on new targeting methods for
F MRI. Herein, we report a strategy, termed as bio-orthogonal metabolic fluorine labeling, for selective cellular
F labeling, which permits in vivo imaging of tumor cells with high specificity. This strategy exploits the display of azido groups on the cell surface via selective uptake and metabolic engineering of tetra-acetylated
-azidoacetylmannosamine (Ac
ManAz) by cancer cells and subsequent rapid and specific bio-orthogonal ligation between azido and cyclootynyl groups to incorporate
F-containing moieties on the surface of cancer cells. We validated the feasibility of this method on the cellular level with A549 and HepG2 cells and further illustrated the application of this method for in vivo deep-tissue visualization of cancer cells with A549 tumor-bearing BALB/c mice using hot spot
F MRI. Our strategy expands the arsenal for targeted
F MRI and provides a promising method for imaging biological targets in living subjects with high tissue penetration and low biological background. |
| Author | Lin, Yaying Luo, Xiangjie Chen, Dongxia Lin, Hongyu Li, Ao Gao, Jinhao Yang, Chaoyong |
| Author_xml | – sequence: 1 givenname: Dongxia surname: Chen fullname: Chen, Dongxia organization: Fujian Provincial Key Laboratory of Chemical Biology, The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, and Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China – sequence: 2 givenname: Yaying surname: Lin fullname: Lin, Yaying organization: Fujian Provincial Key Laboratory of Chemical Biology, The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, and Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China – sequence: 3 givenname: Ao surname: Li fullname: Li, Ao organization: Fujian Provincial Key Laboratory of Chemical Biology, The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, and Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China – sequence: 4 givenname: Xiangjie surname: Luo fullname: Luo, Xiangjie organization: Fujian Provincial Key Laboratory of Chemical Biology, The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, and Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China – sequence: 5 givenname: Chaoyong orcidid: 0000-0002-2374-5342 surname: Yang fullname: Yang, Chaoyong organization: Fujian Provincial Key Laboratory of Chemical Biology, The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, and Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China – sequence: 6 givenname: Jinhao orcidid: 0000-0003-3215-7013 surname: Gao fullname: Gao, Jinhao organization: Fujian Provincial Key Laboratory of Chemical Biology, The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, and Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China – sequence: 7 givenname: Hongyu orcidid: 0000-0002-5675-8537 surname: Lin fullname: Lin, Hongyu organization: Fujian Provincial Key Laboratory of Chemical Biology, The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, and Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/36398367$$D View this record in MEDLINE/PubMed |
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| CitedBy_id | crossref_primary_10_1038_s41467_025_57785_8 crossref_primary_10_1016_j_trac_2024_117607 crossref_primary_10_1021_acs_analchem_4c06942 crossref_primary_10_1002_anie_202313753 crossref_primary_10_1016_j_cej_2024_153747 crossref_primary_10_1016_j_ccr_2024_216148 crossref_primary_10_3892_ijo_2025_5776 crossref_primary_10_1002_ange_202313753 |
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| Snippet | The high resolution, deep penetration, and negligible biological background of
F magnetic resonance imaging (MRI) makes it a potential means for imaging... |
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| SubjectTerms | Animals Fluorides Fluorine Magnetic Resonance Imaging Mice Mice, Inbred BALB C Neoplasms - diagnostic imaging |
| Title | Bio-orthogonal Metabolic Fluorine Labeling Enables Deep-Tissue Visualization of Tumor Cells In Vivo by 19 F Magnetic Resonance Imaging |
| URI | https://www.ncbi.nlm.nih.gov/pubmed/36398367 |
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