Possibilities of optical fluorescence spectral decomposition of meibum

The is study evaluated the possibilities of using optical fluorescence to determine the components of meibum. Meibum samples from 16 volunteers were analyzed using fluorescence spectroscopy in the visible optical range. The meibum spectrum was decomposed into three spectral components corresponding...

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Bibliographic Details
Published in:Vestnik oftal'mologii Vol. 141; no. 5; p. 29
Main Authors: Safonova, T N, Novikov, I A, Zaitseva, G V, Dobretsova, E A, Solodovnikov, V I
Format: Journal Article
Language:English
Russian
Published: Russia (Federation) 2025
Subjects:
Adult
Female
Humans
Keratins - analysis
Lactoferrin - analysis
Male
Meibomian Glands - chemistry
Meibomian Glands - metabolism
Spectrometry, Fluorescence - methods
Tears - chemistry
meibomian glands
keratin
lactoferrin
meibum
fluorescence spectroscopy
flavin
proteomic analysis of meibum
ISSN:0042-465X
Online Access:Get more information
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Summary:The is study evaluated the possibilities of using optical fluorescence to determine the components of meibum. Meibum samples from 16 volunteers were analyzed using fluorescence spectroscopy in the visible optical range. The meibum spectrum was decomposed into three spectral components corresponding to the fluorescence of three reference substances: native keratin, lactoferrin, and riboflavin-mononucleotide. Each meibum sample was characterized by the spectral fraction of each of the three dominant fluorophores. The meibum spectra of 16 studied samples were obtained by fluorescence spectroscopy in the visible optical range under 440 nm laser excitation. Fluorescence of the meibum sample was detected in all cases. Using the proposed algorithm, the relative spectral component of one of the proposed reference substances was calculated for each real spectrum. Fluorescence spectroscopy in the visible optical range, alongside other methods, allows the identification of major compartments constituting the bulk of meibum mass.
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ISSN:0042-465X
DOI:10.17116/oftalma202514105129