Revolutionizing Molecular Cloning: Introducing FastCloneAssist, a Streamlined Python Tool for Optimizing Primer Design in Restriction & Ligation-Independent PCR Cloning

FastCloning, a paradigm shift in PCR cloning, has streamlined the process by eliminating laborious, multi-step traditional methods. This innovative technique, pioneered by Li et al. (2011), utilizes overlapping PCR primers and DpnI digestion for seamless integration of insert DNA into any desired ve...

Full description

Bibliographic Details

Published in:	bioRxiv
Main Authors:	Singh, Pradip Kumar, Donnenberg, Michael S.
Format:	Paper
Language:	English
Published:	Cold Spring Harbor Laboratory 03.07.2024
Edition:	1.1
Subjects:	Bioengineering
ISSN:	2692-8205
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!