Pulsatile dynamics propagate crystalline order in the developing Drosophila eye
Pattern formation in developing tissues often involves self-organization guided by positional information. In most tissues, however, its dynamics, and therefore the underlying logic, remain unknown. Examining self-organized patterning of the fly eye, we combine experiments and modeling to elucidate...
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Cold Spring Harbor Laboratory
04.11.2025
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| Abstract | Pattern formation in developing tissues often involves self-organization guided by positional information. In most tissues, however, its dynamics, and therefore the underlying logic, remain unknown. Examining self-organized patterning of the fly eye, we combine experiments and modeling to elucidate how rows of light-receiving units emerge in the wake of a traveling differentiation front to form a crystal-like array. Live imaging of the proneural factor Atonal reveals unanticipated oscillations at the front, which are produced by the successive activation of two distinct enhancers and associated with pulsatile Notch signaling. Our observations are inconsistent with current models of eye patterning, whereby each row of differentiating cells provides a negative template for the next. Instead, they inform a new relay model in which transient Notch signaling from differentiating cells provides a positive template for the onset of differentiation two rows ahead, conveying both temporal and spatial information to propagate oscillations and crystal-like order. |
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| AbstractList | Pattern formation in developing tissues often involves self-organization guided by positional information. In most tissues, however, its dynamics, and therefore the underlying logic, remain unknown. Examining self-organized patterning of the fly eye, we combine experiments and modeling to elucidate how rows of light-receiving units emerge in the wake of a traveling differentiation front to form a crystal-like array. Live imaging of the proneural factor Atonal reveals unanticipated oscillations at the front, which are produced by the successive activation of two distinct enhancers and associated with pulsatile Notch signaling. Our observations are inconsistent with current models of eye patterning, whereby each row of differentiating cells provides a negative template for the next. Instead, they inform a new relay model in which transient Notch signaling from differentiating cells provides a positive template for the onset of differentiation two rows ahead, conveying both temporal and spatial information to propagate oscillations and crystal-like order. |
| Author | Corson, Francis Luna-Escalante, Juan Tinevez, Jean-Yves Schweisguth, François Mestdagh, Claire Mazouni, Khallil Phan, Minh-Son Couturier, Lydie |
| Author_xml | – sequence: 1 givenname: Lydie surname: Couturier fullname: Couturier, Lydie organization: CNRS UMR3738 – sequence: 2 givenname: Juan surname: Luna-Escalante fullname: Luna-Escalante, Juan organization: Laboratoire de Physique de l’Ecole Normale Supérieure, CNRS, ENS, Université PSL, Sorbonne Université, Université Paris Cité – sequence: 3 givenname: Khallil surname: Mazouni fullname: Mazouni, Khallil organization: CNRS UMR3738 – sequence: 4 givenname: Claire surname: Mestdagh fullname: Mestdagh, Claire organization: CNRS UMR3738 – sequence: 5 givenname: Minh-Son surname: Phan fullname: Phan, Minh-Son organization: CNRS UMR3738 – sequence: 6 givenname: Jean-Yves surname: Tinevez fullname: Tinevez, Jean-Yves organization: Institut Pasteur, Université Paris Cité, IAH – sequence: 7 givenname: François orcidid: 0000-0001-8888-9390 surname: Schweisguth fullname: Schweisguth, François email: fschweis@pasteur.fr organization: CNRS UMR3738 – sequence: 8 givenname: Francis surname: Corson fullname: Corson, Francis email: fschweis@pasteur.fr organization: Center for Studies in Physics and Biology, The Rockefeller University |
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| Cites_doi | 10.1038/s41580-024-00709-z 10.1101/055707 10.1101/2021.07.14.452386 |
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| Notes | Competing Interest Statement: The authors have declared no competing interest. |
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| Title | Pulsatile dynamics propagate crystalline order in the developing Drosophila eye |
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