Optimized husbandry and targeted gene-editing for the cnidarian Nematostella vectensis
Optimized laboratory conditions for research models are crucial for the success of scientific projects. This includes the control of the entire life cycle, access to all developmental stages and maintaining stable physiological conditions. Reducing the life cycle of a research model can also enhance...
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Cold Spring Harbor Laboratory
23.11.2024
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| Abstract | Optimized laboratory conditions for research models are crucial for the success of scientific projects. This includes the control of the entire life cycle, access to all developmental stages and maintaining stable physiological conditions. Reducing the life cycle of a research model can also enhance the access to biological material and speed up genetic tool development. Thus, we optimized the rearing conditions for the sea anemone Nematostella vectensis, a cnidarian research model to study embryonic and post-metamorphic processes, such as regeneration.
We adopted a semi-automated aquaculture system for N. vectensis and developed a dietary protocol optimized for the different life stages. Thereby, we increased spawning efficiencies and post-spawning survival rates, and considerably reduced the overall life cycle down to two months. To further improve the obtention of CRISPR-Cas9 mutants, we optimized the design of sgRNAs leading to full KO animals in F0 polyps using a single sgRNA. Finally, we show that NHEJ-mediated transgene insertion is possible in N. vectensis. In sum our study provides additional resources for the scientific community that uses or will use N. vectensis as a research model.
Optimized life cycle, in combination with efficient gene-editing approaches facilitates the establishment of genetic tools in N. vectensis, an emerging model for environmental stress response, regeneration, and longevity. |
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| AbstractList | Optimized laboratory conditions for research models are crucial for the success of scientific projects. This includes the control of the entire life cycle, access to all developmental stages and maintaining stable physiological conditions. Reducing the life cycle of a research model can also enhance the access to biological material and speed up genetic tool development. Thus, we optimized the rearing conditions for the sea anemone Nematostella vectensis, a cnidarian research model to study embryonic and post-metamorphic processes, such as regeneration.
We adopted a semi-automated aquaculture system for N. vectensis and developed a dietary protocol optimized for the different life stages. Thereby, we increased spawning efficiencies and post-spawning survival rates, and considerably reduced the overall life cycle down to two months. To further improve the obtention of CRISPR-Cas9 mutants, we optimized the design of sgRNAs leading to full KO animals in F0 polyps using a single sgRNA. Finally, we show that NHEJ-mediated transgene insertion is possible in N. vectensis. In sum our study provides additional resources for the scientific community that uses or will use N. vectensis as a research model.
Optimized life cycle, in combination with efficient gene-editing approaches facilitates the establishment of genetic tools in N. vectensis, an emerging model for environmental stress response, regeneration, and longevity. |
| Author | Amiel, Aldine R. Burtin, Maxence Carvalho, João E. Detournay, Olivier Röttinger, Eric |
| Author_xml | – sequence: 1 givenname: João E. orcidid: 0000-0002-7770-4351 surname: Carvalho fullname: Carvalho, João E. organization: Université Côte d’Azur, Federative Research Institute – Marine Resources (IFR MARRES) – sequence: 2 givenname: Maxence surname: Burtin fullname: Burtin, Maxence organization: Université Côte d’Azur, Federative Research Institute – Marine Resources (IFR MARRES) – sequence: 3 givenname: Olivier surname: Detournay fullname: Detournay, Olivier organization: Planktovie SAS – sequence: 4 givenname: Aldine R. orcidid: 0000-0002-3049-2822 surname: Amiel fullname: Amiel, Aldine R. email: eric.rottinger@unice.fr organization: Université Côte d’Azur, Federative Research Institute – Marine Resources (IFR MARRES) – sequence: 5 givenname: Eric orcidid: 0000-0002-2938-6774 surname: Röttinger fullname: Röttinger, Eric email: eric.rottinger@unice.fr organization: Université Côte d’Azur, Federative Research Institute – Marine Resources (IFR MARRES) |
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| ContentType | Paper |
| Copyright | 2023, Posted by Cold Spring Harbor Laboratory |
| Copyright_xml | – notice: 2023, Posted by Cold Spring Harbor Laboratory |
| DBID | FX. |
| DOI | 10.1101/2023.04.14.536874 |
| DatabaseName | bioRxiv |
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| Discipline | Biology |
| EISSN | 2692-8205 |
| Edition | 1.1 |
| ExternalDocumentID | 2023.04.14.536874v1 |
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| IngestDate | Tue Jan 07 19:00:05 EST 2025 |
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| Keywords | Stress-response Life-cycle optimization Regeneration Longevity CRISPR-Cas9 Keys words: Cnidaria |
| Language | English |
| License | This pre-print is available under a Creative Commons License (Attribution-NonCommercial-NoDerivs 4.0 International), CC BY-NC-ND 4.0, as described at http://creativecommons.org/licenses/by-nc-nd/4.0 |
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| Notes | Competing Interest Statement: OD and ER own shares in, and OD is employee of Planktovie SAS, the supplier of rotifers and RG complete used in this research. The authors report no other competing interest. |
| ORCID | 0000-0002-2938-6774 0000-0002-3049-2822 0000-0002-7770-4351 |
| OpenAccessLink | https://www.biorxiv.org/content/10.1101/2023.04.14.536874 |
| PageCount | 47 |
| ParticipantIDs | biorxiv_primary_2023_04_14_536874 |
| PublicationCentury | 2000 |
| PublicationDate | 2024-11-23 |
| PublicationDateYYYYMMDD | 2024-11-23 |
| PublicationDate_xml | – month: 11 year: 2024 text: 2024-11-23 day: 23 |
| PublicationDecade | 2020 |
| PublicationTitle | bioRxiv |
| PublicationYear | 2024 |
| Publisher | Cold Spring Harbor Laboratory |
| Publisher_xml | – name: Cold Spring Harbor Laboratory |
| References | Srivastava, Goldstein, Srivastava (2023.04.14.536874v1.86) 2022 Dhont, Dierckens, Støttrup, Van Stappen, Wille, Sorgeloos, Allan, Burnell (2023.04.14.536874v1.25) 2013 Lieschke, Currie (2023.04.14.536874v1.62) 2007; 8 Arboleda, Hartenstein, Martinez, Reichert, Sen, Sprecher, Bailly (2023.04.14.536874v1.9) 2018; 40 Soto-Àngel, Nordmann, Sturm, Sachkova, Pang, Burkhardt (2023.04.14.536874v1.85) 2022 Brix, Cardwell, Adams (2023.04.14.536874v1.15) 2003; 54 Gordon, Roth, Caicci, Manni, Shenkar (2023.04.14.536874v1.35) 2020; 17 Davis, Zarowiecki, Arnaboldi, Becerra, Cain, Chan, Chen, Cho, da Veiga Beltrame, Diamantakis (2023.04.14.536874v1.24) 2022; 220 Admoni, Kozlovski, Lewandowska, Moran (2023.04.14.536874v1.1) 2020; 11 Bradford, Van Slyke, Ruzicka, Singer, Eagle, Fashena, Howe, Frazer, Martin, Paddock (2023.04.14.536874v1.14) 2022; 220 Gramates, Agapite, Attrill, Calvi, Crosby, dos Santos, Goodman, Goutte-Gattat, Jenkins, Kaufman (2023.04.14.536874v1.36) 2022; 220 Chourrout, Delsuc, Chourrout, Edvardsen, Rentzsch, Renfer, Jensen, Zhu, de Jong, Steele (2023.04.14.536874v1.21) 2006; 442 Dabrowski, Miller (2023.04.14.536874v1.23) 2018; 15 Steger, Cole, Denner, Lebedeva, Genikhovich, Ries, Reischl, Taudes, Lassnig, Technau (2023.04.14.536874v1.88) 2022; 40 Valverde, Labella, Borrego, Castro (2023.04.14.536874v1.93) 2019; 11 Lechable, Jan, Duchene, Uveira, Weissbourd, Gissat, Collet, Gilletta, Chevalier, Leclère (2023.04.14.536874v1.59) 2020; 9 Wikramanayake, Hong, Lee, Pang, Byrum, Bince, Xu, Martindale (2023.04.14.536874v1.97) 2003; 426 Carvalho, Lahaye, Schubert (2023.04.14.536874v1.17) 2017; 61 Amiel, Johnston, Nedoncelle, Warner, Ferreira, Röttinger (2023.04.14.536874v1.5) 2015; 16 Yoshimi, Oka, Miyasaka, Kotani, Yasumura, Uno, Hattori, Tanigawa, Sato, Oya (2023.04.14.536874v1.99) 2021; 140 Stefanik, Friedman, Finnerty (2023.04.14.536874v1.87) 2013; 8 Wijesena, Simmons, Martindale (2023.04.14.536874v1.96) 2017; 114 Lawrence, Sanders, Henry (2023.04.14.536874v1.55) 2012; 9 Richards, Rentzsch (2023.04.14.536874v1.77) 2014; 141 Williams (2023.04.14.536874v1.98) 1983 Baldassarre, Ying, Reitzel, Franzenburg, Fraune (2023.04.14.536874v1.11) 2022; 13 Warner, Guerlais, Amiel, Johnston, Nedoncelle, Röttinger (2023.04.14.536874v1.95) 2018; 145 Babonis, Enjolras, Reft, Foster, Hugosson, Ryan, Daly, Martindale (2023.04.14.536874v1.10) 2021 Kassmer, Nourizadeh, De Tomaso (2023.04.14.536874v1.50) 2019; 448 Ormestad, Martindale, Röttinger (2023.04.14.536874v1.69) 2011; 2 Kusserow, Pang, Sturm, Hrouda, Lentfer, Schmidt, Technau, von Haeseler, Hobmayer, Martindale (2023.04.14.536874v1.53) 2005; 433 Chen, McKinney, Ellington, Gibson (2023.04.14.536874v1.19) 2020; 9 Passamaneck, Martindale (2023.04.14.536874v1.72) 2012; 12 Layden, Rentzsch, Röttinger (2023.04.14.536874v1.56) 2016; 5 Elran, Raam, Kraus, Brekhman, Sher, Plaschkes, Chalifa-Caspi, Lotan (2023.04.14.536874v1.29) 2014; 23 Paix, Basu, Steenbergen, Singh, Prevedel, Ikmi (2023.04.14.536874v1.70) 2023; 120 Tkavc, Ausec, Oren, Gunde-Cimerman (2023.04.14.536874v1.91) 2011; 77 Angilletta, Steury, Sears (2023.04.14.536874v1.8) 2004; 44 Holland, Li, Carroll, Stricker (2023.04.14.536874v1.44) 2021 Ivankovic, Haneckova, Thommen, Grohme, Vila-Farré, Werner, Rink (2023.04.14.536874v1.48) 2019; 146 Reitzel, Burton, Krone, Finnerty (2023.04.14.536874v1.73) 2007; 126 Lawrence (2023.04.14.536874v1.54) 2007; 269 Bossert, Dunn, Thomsen (2023.04.14.536874v1.13) 2013; 242 Havrilak, Faltine-Gonzalez, Wen, Fodera, Simpson, Magie, Layden (2023.04.14.536874v1.39) 2017; 431 Lebedeva, Boström, Mörsdorf, Niedermoser, Genikhovich, Adameyko, Genikhovich (2023.04.14.536874v1.57) 2022 Nakanishi, Martindale (2023.04.14.536874v1.67) 2018; 7 Murabe, Okumura, Chiba, Hosoda, Ikegami, Kishimoto, Carroll, Stricker (2023.04.14.536874v1.66) 2021 Reuven, Rinsky, Brekhman, Malik, Levy, Lotan (2023.04.14.536874v1.76) 2021; 11 Ehrlich, Gahan, Rentzsch, Kühn (2023.04.14.536874v1.27) 2022; 225 Ikmi, Gibson (2023.04.14.536874v1.45) 2010; 5 Momose, Concordet (2023.04.14.536874v1.65) 2016; 30 Mehta, Singh (2023.04.14.536874v1.63) 2019; 453 Steinmetz, Aman, Kraus, Technau (2023.04.14.536874v1.89) 2017; 1 Renfer, Amon-Hassenzahl, Steinmetz, Technau (2023.04.14.536874v1.75) 2010; 107 Hand, Uhlinger (2023.04.14.536874v1.38) 1994; 17 Kraus, Aman, Technau, Genikhovich (2023.04.14.536874v1.52) 2016; 7 Zimmermann, Robb, Genikhovich, Fropf, Weilguny, He, Chen, Lovegrove-Walsh, Hill, Chen (2023.04.14.536874v1.102) 2022; 2020 Doudna, Charpentier (2023.04.14.536874v1.26) 2014; 346 Röttinger, Dahlin, Martindale (2023.04.14.536874v1.79) 2012; 8 Sunagar, Columbus-Shenkar, Fridrich, Gutkovich, Aharoni, Moran (2023.04.14.536874v1.90) 2018; 16 Fei, Lou, Knapp, Murawala, Gerber, Taniguchi, Nowoshilow, Khattak, Tanaka (2023.04.14.536874v1.30) 2018; 13 He, del Viso, Chen, Ikmi, Kroesen, Gibson (2023.04.14.536874v1.40) 2018; 361 Lieber (2023.04.14.536874v1.61) 2010; 79 Silva, Poirot, Galetto, Smith, Montoya, Duchateau, Paques (2023.04.14.536874v1.84) 2011; 11 Fritzenwanker, Genikhovich, Kraus, Technau (2023.04.14.536874v1.32) 2007; 310 Höijer, Emmanouilidou, Östlund, van Schendel, Bozorgpana, Tijsterman, Feuk, Gyllensten, den Hoed, Ameur (2023.04.14.536874v1.43) 2022; 13 Roux, Salis, Lee, Besseau, Laudet (2023.04.14.536874v1.80) 2020; 11 Ikmi, Steenbergen, Anzo, McMullen, Stokkermans, Ellington, Gibson (2023.04.14.536874v1.47) 2020; 11 Hill, Chen, del Viso, Ellington, He, Karabulut, Paulson, Gibson, Blanchoud, Galliot (2023.04.14.536874v1.42) 2022 Johnston, Warner, Amiel, Nedoncelle, Carvalho, Röttinger (2023.04.14.536874v1.49) 2021 Klein, Frazier, Readdean, Lucas, Diaz-Jimenez, Sogin, Ruff, Echeverri (2023.04.14.536874v1.51) 2021; 9 Tournière, Dolan, Richards, Sunagar, Columbus-Shenkar, Moran, Rentzsch (2023.04.14.536874v1.92) 2020; 30 Lee, Kumburegama, Marlow, Martindale, Wikramanayake (2023.04.14.536874v1.60) 2007; 310 Chames, Epinat, Guillier, Patin, Lacroix, Pâques (2023.04.14.536874v1.18) 2005; 33 Hand, Uhlinger (2023.04.14.536874v1.37) 1992; 182 Seleit, Aulehla, Paix (2023.04.14.536874v1.82) 2021; 10 Servetnick, Steinworth, Babonis, Simmons, Salinas-Saavedra, Martindale (2023.04.14.536874v1.83) 2017; 144 Zhang, Li, Li, Chen, Arakaki, Botimer, Baylink, Zhang, Wen, Fu (2023.04.14.536874v1.101) 2017; 18 Busengdal, Rentzsch (2023.04.14.536874v1.16) 2017; 431 Gahan, Kouzel, Jansen, Burkhardt, Rentzsch (2023.04.14.536874v1.33) 2022; 13 Genikhovich, Technau (2023.04.14.536874v1.34) 2009; 2009 Best, Adatto, Cockington, James, Lawrence (2023.04.14.536874v1.12) 2010; 7 Al-Shaer, Havrilak, Layden (2023.04.14.536874v1.2) 2021 Ambrosone, Marchesano, Mazzarella, Tortiglione (2023.04.14.536874v1.4) 2014; 8 Reitzel, Sullivan, Traylor-knowles, Finnerty (2023.04.14.536874v1.74) 2008; 214 Röttinger (2023.04.14.536874v1.78) 2021; 10 Miyaoka, Berman, Cooper, Mayerl, Chan, Zhang, Karlin-Neumann, Conklin (2023.04.14.536874v1.64) 2016; 6 Nakanishi, Renfer, Technau, Rentzsch (2023.04.14.536874v1.68) 2012; 139 Varshney, Carrington, Pei, Bishop, Chen, Fan, Xu, Jones, LaFave, Ledin (2023.04.14.536874v1.94) 2016; 11 Amiel, Foucher, Ferreira, Röttinger (2023.04.14.536874v1.6) 2019 Henry, Lesoway, Perry (2023.04.14.536874v1.41) 2020; 18 Amiel, Michel, Carvalho, Shkreli, Petit, Röttinger (2023.04.14.536874v1.7) 2021; 37 Elliott, Richardson, Winderbaum, Nickoloff, Jasin (2023.04.14.536874v1.28) 1998; 18 Ikmi, Delventhal, Gibson, McKinney (2023.04.14.536874v1.46) 2014; 5 Al-Shaer, Leach, Baban, Yagodich, Gibson, Layden (2023.04.14.536874v1.3) 2023 Lebouvier, Miramón-Puértolas, Steinmetz (2023.04.14.536874v1.58) 2022; 2022 Fritzenwanker, Technau (2023.04.14.536874v1.31) 2002; 212 Schneider, Rasband, Eliceiri (2023.04.14.536874v1.81) 2012; 9 Pan, Dahms, Hwang, Souissi (2023.04.14.536874v1.71) 2022; 9 Chiruvella, Liang, Wilson (2023.04.14.536874v1.20) 2013; 5 Cook, Chenevert, Larsson, Arendt, Houliston, Lénárt (2023.04.14.536874v1.22) 2016; 27 Zang, Nakanishi (2023.04.14.536874v1.100) 2020; 11 |
| References_xml | – volume: 431 start-page: 59 year: 2017 end-page: 68 ident: 2023.04.14.536874v1.16 article-title: Unipotent progenitors contribute to the generation of sensory cell types in the nervous system of the cnidarian Nematostella vectensis publication-title: Developmental Biology – volume: 431 start-page: 336 year: 2017 end-page: 346 ident: 2023.04.14.536874v1.39 article-title: Characterization of NvLWamide-like neurons reveals stereotypy in Nematostella nerve net development publication-title: Developmental Biology – start-page: 658930 year: 2021 ident: 2023.04.14.536874v1.49 publication-title: Whole body regeneration deploys a rewired embryonic gene regulatory network logic – volume: 220 start-page: iyac016 year: 2022 ident: 2023.04.14.536874v1.14 article-title: Zebrafish information network, the knowledgebase for Danio rerio research publication-title: Genetics – volume: 310 start-page: 169 year: 2007 end-page: 186 ident: 2023.04.14.536874v1.60 article-title: Asymmetric developmental potential along the animal–vegetal axis in the anthozoan cnidarian, Nematostella vectensis, is mediated by Dishevelled publication-title: Developmental Biology – volume: 54 start-page: 169 year: 2003 end-page: 175 ident: 2023.04.14.536874v1.15 article-title: Chronic toxicity of arsenic to the Great Salt Lake brine shrimp, Artemia franciscana publication-title: Ecotoxicology and Environmental Safety – volume: 9 year: 2021 ident: 2023.04.14.536874v1.51 article-title: Common environmental pollutants negatively affect development and regeneration in the sea anemone Nematostella vectensis holobiont publication-title: Frontiers in Ecology and Evolution – volume: 220 start-page: iyac035 year: 2022 ident: 2023.04.14.536874v1.36 article-title: FlyBase: a guided tour of highlighted features publication-title: Genetics – start-page: 153 year: 2022 end-page: 172 ident: 2023.04.14.536874v1.86 publication-title: Current Topics in Developmental Biology – volume: 79 start-page: 181 year: 2010 end-page: 211 ident: 2023.04.14.536874v1.61 article-title: The mechanism of double-strand DNA break repair by the nonhomologous DNA end-joining pathway publication-title: Annual Review of Biochemistry – volume: 8 start-page: e1003164 year: 2012 ident: 2023.04.14.536874v1.79 article-title: A framework for the establishment of a cnidarian gene regulatory network for “endomesoderm” specification: The inputs of ß-Catenin/TCF signaling publication-title: PLOS Genetics – volume: 44 start-page: 498 year: 2004 end-page: 509 ident: 2023.04.14.536874v1.8 article-title: Temperature, growth rate, and body size in ectotherms: Fitting pieces of a life-history puzzle publication-title: Integrative and Comparative Biology – volume: 426 start-page: 446 year: 2003 end-page: 450 ident: 2023.04.14.536874v1.97 article-title: An ancient role for nuclear β-catenin in the evolution of axial polarity and germ layer segregation publication-title: Nature – volume: 182 start-page: 169 year: 1992 end-page: 176 ident: 2023.04.14.536874v1.37 article-title: The culture, sexual and asexual reproduction, and growth of the sea anemone Nematostella vectensis publication-title: The Biological Bulletin – volume: 61 start-page: 773 year: 2017 end-page: 783 ident: 2023.04.14.536874v1.17 article-title: Keeping amphioxus in the laboratory: an update on available husbandry methods publication-title: Int. J. Dev. Biol – volume: 11 start-page: 4399 year: 2020 ident: 2023.04.14.536874v1.47 article-title: Feeding-dependent tentacle development in the sea anemone Nematostella vectensis publication-title: Nat Commun – volume: 10 start-page: 2692 year: 2021 ident: 2023.04.14.536874v1.78 article-title: Nematostella vectensis, an emerging model for deciphering the molecular and cellular mechanisms underlying whole-body regeneration publication-title: Cells – volume: 214 start-page: 233 year: 2008 end-page: 254 ident: 2023.04.14.536874v1.74 article-title: Genomic survey of candidate stress-response genes in the estuarine anemone Nematostella vectensis publication-title: The Biological Bulletin – volume: 11 start-page: 15451 year: 2021 ident: 2023.04.14.536874v1.76 article-title: Cellular pathways during spawning induction in the starlet sea anemone Nematostella vectensis publication-title: Sci Rep – start-page: 157 year: 2013 end-page: 202 ident: 2023.04.14.536874v1.25 publication-title: Advances in Aquaculture Hatchery Technology – volume: 23 start-page: 4722 year: 2014 end-page: 4736 ident: 2023.04.14.536874v1.29 article-title: Early and late response of Nematostella vectensis transcriptome to heavy metals publication-title: Molecular Ecology – volume: 18 start-page: 46 year: 2020 ident: 2023.04.14.536874v1.41 article-title: An automated aquatic rack system for rearing marine invertebrates publication-title: BMC Biology – year: 2019 ident: 2023.04.14.536874v1.6 article-title: Synergic coordination of stem cells is required to induce a regenerative response in anthozoan cnidarians publication-title: bioRxiv – volume: 16 start-page: 28449 year: 2015 end-page: 28471 ident: 2023.04.14.536874v1.5 article-title: Characterization of morphological and cellular events underlying oral regeneration in the sea anemone, Nematostella vectensis publication-title: International Journal of Molecular Sciences – volume: 11 start-page: 20 year: 2020 ident: 2023.04.14.536874v1.80 article-title: Anemonefish, a model for Eco-Evo-Devo publication-title: EvoDevo – volume: 5 start-page: e11807 year: 2010 ident: 2023.04.14.536874v1.45 article-title: Identification and in vivo characterization of NvFP-7R, a developmentally regulated red fluorescent protein of Nematostella vectensis publication-title: PLOS ONE – volume: 7 start-page: 11694 year: 2016 ident: 2023.04.14.536874v1.52 article-title: Pre-bilaterian origin of the blastoporal axial organizer publication-title: Nat Commun – volume: 448 start-page: 271 year: 2019 end-page: 278 ident: 2023.04.14.536874v1.50 article-title: Cellular and molecular mechanisms of regeneration in colonial and solitary Ascidians publication-title: Developmental Biology – volume: 9 year: 2020 ident: 2023.04.14.536874v1.59 article-title: An improved whole life cycle culture protocol for the hydrozoan genetic model Clytia hemisphaerica publication-title: Biology Open – volume: 27 start-page: 882 year: 2016 end-page: 887 ident: 2023.04.14.536874v1.22 article-title: Old knowledge and new technologies allow rapid development of model organisms publication-title: MBoC – volume: 361 start-page: 1377 year: 2018 end-page: 1380 ident: 2023.04.14.536874v1.40 article-title: An axial Hox code controls tissue segmentation and body patterning in Nematostella vectensis publication-title: Science – volume: 11 year: 2020 ident: 2023.04.14.536874v1.100 article-title: Expression analysis of cnidarian-specific neuropeptides in a sea anemone unveils an apical-organ-associated nerve net that disintegrates at metamorphosis publication-title: Frontiers in Endocrinology – volume: 17 start-page: 19 year: 2020 ident: 2023.04.14.536874v1.35 article-title: Spawning induction, development and culturing of the solitary ascidian Polycarpa mytiligera, an emerging model for regeneration studies publication-title: Frontiers in Zoology – volume: 18 start-page: 35 year: 2017 ident: 2023.04.14.536874v1.101 article-title: Efficient precise knockin with a double cut HDR donor after CRISPR/Cas9-mediated double-stranded DNA cleavage publication-title: Genome Biology – volume: 141 start-page: 4681 year: 2014 end-page: 4689 ident: 2023.04.14.536874v1.77 article-title: Transgenic analysis of a SoxB gene reveals neural progenitor cells in the cnidarian Nematostella vectensis publication-title: Development – volume: 145 year: 2018 ident: 2023.04.14.536874v1.95 article-title: NvERTx: a gene expression database to compare embryogenesis and regeneration in the sea anemone Nematostella vectensis publication-title: Development – volume: 5 start-page: a012757 year: 2013 ident: 2023.04.14.536874v1.20 article-title: Repair of double-strand breaks by end joining publication-title: Cold Spring Harb Perspect Biol – volume: 220 start-page: iyac003 year: 2022 ident: 2023.04.14.536874v1.24 article-title: WormBase in 2022—data, processes, and tools for analyzing Caenorhabditis elegans publication-title: Genetics – volume: 120 start-page: e2215958120 year: 2023 ident: 2023.04.14.536874v1.70 article-title: Endogenous tagging of multiple cellular components in the sea anemone Nematostella vectensis publication-title: Proceedings of the National Academy of Sciences – volume: 8 start-page: 353 year: 2007 end-page: 367 ident: 2023.04.14.536874v1.62 article-title: Animal models of human disease: zebrafish swim into view publication-title: Nat Rev Genet – volume: 1 start-page: 1535 year: 2017 end-page: 1542 ident: 2023.04.14.536874v1.89 article-title: Gut-like ectodermal tissue in a sea anemone challenges germ layer homology publication-title: Nature Ecology & Evolution – volume: 17 start-page: 501 year: 1994 end-page: 508 ident: 2023.04.14.536874v1.38 article-title: The unique, widely distributed, estuarine sea anemone, Nematostella vectensis Stephenson: A review, new facts, and questions publication-title: Estuaries – start-page: 437 year: 2022 end-page: 465 ident: 2023.04.14.536874v1.42 publication-title: Whole-Body Regeneration: Methods and Protocols – volume: 9 start-page: 140 year: 2012 end-page: 146 ident: 2023.04.14.536874v1.55 article-title: Methods for culturing saltwater rotifers (Brachionus plicatilis) for rearing larval zebrafish publication-title: Zebrafish – year: 2023 ident: 2023.04.14.536874v1.3 article-title: Environmental and molecular regulation of asexual reproduction in the sea anemone publication-title: Nematostella vectensis – volume: 225 start-page: jeb243717 year: 2022 ident: 2023.04.14.536874v1.27 article-title: TRPM2 causes sensitization to oxidative stress but attenuates high-temperature injury in the sea anemone Nematostella vectensis publication-title: Journal of Experimental Biology – volume: 453 start-page: 111 year: 2019 end-page: 129 ident: 2023.04.14.536874v1.63 article-title: Insights into regeneration tool box: An animal model approach publication-title: Developmental Biology – volume: 11 start-page: 11 year: 2011 end-page: 27 ident: 2023.04.14.536874v1.84 article-title: Meganucleases and other tools for targeted genome engineering: Perspectives and challenges for gene therapy publication-title: Current Gene Therapy – volume: 11 start-page: 506 year: 2019 ident: 2023.04.14.536874v1.93 article-title: Artemia spp., a susceptible host and vector for lymphocystis disease virus publication-title: Viruses – volume: 212 start-page: 99 year: 2002 end-page: 103 ident: 2023.04.14.536874v1.31 article-title: Induction of gametogenesis in the basal cnidarian Nematostella vectensis (Anthozoa) publication-title: Dev Genes Evol – start-page: 49 year: 2021 end-page: 68 ident: 2023.04.14.536874v1.66 publication-title: Developmental Biology of the Sea Urchin and Other Marine Invertebrates: Methods and Protocols – volume: 10 start-page: e75050 year: 2021 ident: 2023.04.14.536874v1.82 article-title: Endogenous protein tagging in medaka using a simplified CRISPR/Cas9 knock-in approach publication-title: eLife – start-page: 43 year: 1983 end-page: 46 ident: 2023.04.14.536874v1.98 article-title: Starlet sea anemone publication-title: IUCN Invertebrate Red Data Book – year: 2021 ident: 2023.04.14.536874v1.10 article-title: Knockout of a single Sox gene resurrects an ancestral cell type in the sea anemone publication-title: Nematostella vectensis – volume: 8 start-page: 916 year: 2013 end-page: 923 ident: 2023.04.14.536874v1.87 article-title: Collecting, rearing, spawning and inducing regeneration of the starlet sea anemone, Nematostella vectensis publication-title: Nat Protoc – year: 2022 ident: 2023.04.14.536874v1.57 publication-title: β-catenin-dependent endomesoderm specification appears to be a Bilateria-specific co-option – volume: 140 start-page: 277 year: 2021 end-page: 287 ident: 2023.04.14.536874v1.99 article-title: Combi-CRISPR: combination of NHEJ and HDR provides efficient and precise plasmid-based knock-ins in mice and rats publication-title: Hum Genet – volume: 77 start-page: 310 year: 2011 end-page: 321 ident: 2023.04.14.536874v1.91 article-title: Bacteria associated with Artemia spp. along the salinity gradient of the solar salterns at Eilat (Israel) publication-title: FEMS Microbiology Ecology – volume: 144 start-page: 2951 year: 2017 end-page: 2960 ident: 2023.04.14.536874v1.83 article-title: Cas9-mediated excision of Nematostella brachyury disrupts endoderm development, pharynx formation and oral-aboral patterning publication-title: Development – volume: 9 start-page: 671 year: 2012 end-page: 675 ident: 2023.04.14.536874v1.81 article-title: NIH Image to ImageJ: 25 years of image analysis publication-title: Nat Meth – volume: 18 start-page: 93 year: 1998 end-page: 101 ident: 2023.04.14.536874v1.28 article-title: Gene conversion tracts from double-strand break repair in mammalian cells publication-title: Molecular and Cellular Biology – volume: 114 start-page: E5608 year: 2017 end-page: E5615 ident: 2023.04.14.536874v1.96 article-title: Antagonistic BMP–cWNT signaling in the cnidarian Nematostella vectensis reveals insight into the evolution of mesoderm publication-title: Proceedings of the National Academy of Sciences – volume: 30 start-page: 4473 year: 2020 end-page: 4489 ident: 2023.04.14.536874v1.92 article-title: NvPOU4/Brain3 functions as a terminal selector gene in the nervous system of the Cnidarian Nematostella vectensis publication-title: Cell Reports – volume: 30 start-page: 55 year: 2016 end-page: 65 ident: 2023.04.14.536874v1.65 article-title: Diving into marine genomics with CRISPR/Cas9 systems publication-title: Marine Genomics – volume: 9 year: 2022 ident: 2023.04.14.536874v1.71 article-title: Recent trends in live feeds for marine larviculture: A mini review publication-title: Frontiers in Marine Science – volume: 139 start-page: 347 year: 2012 end-page: 357 ident: 2023.04.14.536874v1.68 article-title: Nervous systems of the sea anemone Nematostella vectensis are generated by ectoderm and endoderm and shaped by distinct mechanisms publication-title: Development – volume: 146 start-page: dev167684 year: 2019 ident: 2023.04.14.536874v1.48 article-title: Model systems for regeneration: planarians publication-title: Development – volume: 12 start-page: 34 year: 2012 ident: 2023.04.14.536874v1.72 article-title: Cell proliferation is necessary for the regeneration of oral structures in the anthozoan cnidarian Nematostella vectensis publication-title: BMC Developmental Biology – volume: 13 start-page: 465 year: 2022 ident: 2023.04.14.536874v1.33 article-title: Histone demethylase Lsd1 is required for the differentiation of neural cells in Nematostella vectensis publication-title: Nat Commun – volume: 40 start-page: 1800107 year: 2018 ident: 2023.04.14.536874v1.9 article-title: An emerging system to study photosymbiosis, brain regeneration, chronobiology, and behavior: The marine Acoel Symsagittifera roscoffensis publication-title: BioEssays – volume: 242 start-page: 1320 year: 2013 end-page: 1331 ident: 2023.04.14.536874v1.13 article-title: A staging system for the regeneration of a polyp from the aboral physa of the anthozoan Cnidarian Nematostella vectensis publication-title: Developmental Dynamics – volume: 126 start-page: 99 year: 2007 end-page: 112 ident: 2023.04.14.536874v1.73 article-title: Comparison of developmental trajectories in the starlet sea anemone Nematostella vectensis: embryogenesis, regeneration, and two forms of asexual fission publication-title: Invertebrate Biology – year: 2021 ident: 2023.04.14.536874v1.2 publication-title: Handbook of Marine Model Organisms in Experimental Biology – volume: 5 start-page: 408 year: 2016 end-page: 428 ident: 2023.04.14.536874v1.56 article-title: The rise of the starlet sea anemone Nematostella vectensis as a model system to investigate development and regeneration publication-title: WIREs Dev Biol – volume: 7 start-page: e39742 year: 2018 ident: 2023.04.14.536874v1.67 article-title: CRISPR knockouts reveal an endogenous role for ancient neuropeptides in regulating developmental timing in a sea anemone publication-title: eLife – volume: 310 start-page: 264 year: 2007 end-page: 279 ident: 2023.04.14.536874v1.32 article-title: Early development and axis specification in the sea anemone Nematostella vectensis publication-title: Developmental Biology – volume: 9 start-page: e54573 year: 2020 ident: 2023.04.14.536874v1.19 article-title: Hedgehog signaling is required for endomesodermal patterning and germ cell development in the sea anemone Nematostella vectensis publication-title: eLife – volume: 269 start-page: 1 year: 2007 end-page: 20 ident: 2023.04.14.536874v1.54 article-title: The husbandry of zebrafish (Danio rerio): A review publication-title: Aquaculture – volume: 37 start-page: 167 year: 2021 end-page: 177 ident: 2023.04.14.536874v1.7 article-title: The sea anemone Nematostella vectensis, an emerging model for biomedical research: Mechano-sensitivity, extreme regeneration and longevity publication-title: Med Sci (Paris – volume: 8 start-page: 508 year: 2014 end-page: 520 ident: 2023.04.14.536874v1.4 article-title: Nanotoxicology using the sea anemone Nematostella vectensis: from developmental toxicity to genotoxicology publication-title: Nanotoxicology – volume: 2009 year: 2009 ident: 2023.04.14.536874v1.34 article-title: Induction of spawning in the starlet sea anemone Nematostella vectensis, in vitro fertilization of gametes, and dejellying of zygotes publication-title: Cold Spring Harb Protoc – start-page: 1 year: 2021 end-page: 29 ident: 2023.04.14.536874v1.44 publication-title: Developmental Biology of the Sea Urchin and Other Marine Invertebrates: Methods and Protocols – volume: 2 start-page: 17 year: 2011 ident: 2023.04.14.536874v1.69 article-title: A comparative gene expression database for invertebrates publication-title: EvoDevo – volume: 11 start-page: 1081 year: 2020 ident: 2023.04.14.536874v1.1 article-title: TATA Binding Protein (TBP) promoter drives ubiquitous expression of marker transgene in the adult sea anemone Nematostella vectensis publication-title: Genes – volume: 13 start-page: 2908 year: 2018 end-page: 2943 ident: 2023.04.14.536874v1.30 article-title: Application and optimization of CRISPR–Cas9-mediated genome engineering in axolotl (Ambystoma mexicanum) publication-title: Nat Protoc – volume: 16 start-page: 108 year: 2018 ident: 2023.04.14.536874v1.90 article-title: Cell type-specific expression profiling unravels the development and evolution of stinging cells in sea anemone publication-title: BMC Biology – volume: 6 start-page: 23549 year: 2016 ident: 2023.04.14.536874v1.64 article-title: Systematic quantification of HDR and NHEJ reveals effects of locus, nuclease, and cell type on genome-editing publication-title: Sci Rep – volume: 40 start-page: 111370 year: 2022 ident: 2023.04.14.536874v1.88 article-title: Single-cell transcriptomics identifies conserved regulators of neuroglandular lineages publication-title: Cell Reports – volume: 13 start-page: 3804 year: 2022 ident: 2023.04.14.536874v1.11 article-title: Microbiota mediated plasticity promotes thermal adaptation in the sea anemone Nematostella vectensis publication-title: Nat Commun – volume: 107 start-page: 104 year: 2010 end-page: 108 ident: 2023.04.14.536874v1.75 article-title: A muscle-specific transgenic reporter line of the sea anemone, Nematostella vectensis publication-title: Proceedings of the National Academy of Sciences – volume: 433 start-page: 156 year: 2005 end-page: 160 ident: 2023.04.14.536874v1.53 article-title: Unexpected complexity of the Wnt gene family in a sea anemone publication-title: Nature – volume: 13 start-page: 627 year: 2022 ident: 2023.04.14.536874v1.43 article-title: CRISPR-Cas9 induces large structural variants at on-target and off-target sites in vivo that segregate across generations publication-title: Nat Commun – volume: 11 start-page: 2357 year: 2016 end-page: 2375 ident: 2023.04.14.536874v1.94 article-title: A high-throughput functional genomics workflow based on CRISPR/Cas9-mediated targeted mutagenesis in zebrafish publication-title: Nat Protoc – volume: 2022 start-page: 01 year: 2022 ident: 2023.04.14.536874v1.58 publication-title: Evolutionary conserved aspects of animal nutrient uptake and transport in sea anemone vitellogenesis – volume: 2020 start-page: 10 year: 2022 ident: 2023.04.14.536874v1.102 publication-title: Sea anemone genomes reveal ancestral metazoan chromosomal macrosynteny – volume: 5 start-page: 5486 year: 2014 ident: 2023.04.14.536874v1.46 article-title: TALEN and CRISPR/Cas9-mediated genome editing in the early-branching metazoan Nematostella vectensis publication-title: Nature Communications – volume: 33 start-page: e178 year: 2005 ident: 2023.04.14.536874v1.18 article-title: In vivo selection of engineered homing endonucleases using double-strand break induced homologous recombination publication-title: Nucleic Acids Research – volume: 7 start-page: 289 year: 2010 end-page: 295 ident: 2023.04.14.536874v1.12 article-title: A novel method for rearing first-feeding larval zebrafish: Polyculture with type L saltwater rotifers (Brachionus plicatilis) publication-title: Zebrafish – volume: 346 start-page: 1258096 year: 2014 ident: 2023.04.14.536874v1.26 article-title: The new frontier of genome engineering with CRISPR-Cas9 publication-title: Science – volume: 15 start-page: 295 year: 2018 end-page: 309 ident: 2023.04.14.536874v1.23 article-title: Contested paradigm in raising Zebrafish (Danio rerio) publication-title: Zebrafish – year: 2022 ident: 2023.04.14.536874v1.85 publication-title: Stable laboratory culture system for the ctenophore Mnemiopsis leidyi – volume: 442 start-page: 684 year: 2006 end-page: 687 ident: 2023.04.14.536874v1.21 article-title: Minimal ProtoHox cluster inferred from bilaterian and cnidarian Hox complements publication-title: Nature |
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| Title | Optimized husbandry and targeted gene-editing for the cnidarian Nematostella vectensis |
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