Additional analyses exploring the hypothesized transdifferentiation of plasmablasts to developing neutrophils in severe COVID-19
Abstract We thank Alquicira-Hernandez et al. for their reanalysis of our single-cell transcriptomic dataset profiling peripheral immune responses to severe COVID-19. We agree that careful analysis of single-cell sequencing data is important for generating cogent hypotheses but find several aspects o...
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Cold Spring Harbor
Cold Spring Harbor Laboratory Press
16.10.2020
Cold Spring Harbor Laboratory |
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| Abstract | Abstract We thank Alquicira-Hernandez et al. for their reanalysis of our single-cell transcriptomic dataset profiling peripheral immune responses to severe COVID-19. We agree that careful analysis of single-cell sequencing data is important for generating cogent hypotheses but find several aspects of their criticism of our analysis to be problematic. Here we respond briefly to misunderstandings and inaccuracies in their commentary that may have led to misinformed interpretation of our results. Competing Interest Statement The authors have declared no competing interest. |
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| AbstractList | We thank Alquicira-Hernandez et al. for their reanalysis of our single-cell transcriptomic dataset profiling peripheral immune responses to severe COVID-19. We agree that careful analysis of single-cell sequencing data is important for generating cogent hypotheses but find several aspects of their criticism of our analysis to be problematic. Here we respond briefly to misunderstandings and inaccuracies in their commentary that may have led to misinformed interpretation of our results. Abstract We thank Alquicira-Hernandez et al. for their reanalysis of our single-cell transcriptomic dataset profiling peripheral immune responses to severe COVID-19. We agree that careful analysis of single-cell sequencing data is important for generating cogent hypotheses but find several aspects of their criticism of our analysis to be problematic. Here we respond briefly to misunderstandings and inaccuracies in their commentary that may have led to misinformed interpretation of our results. Competing Interest Statement The authors have declared no competing interest. |
| Author | Martin, Beth A Blish, Catherine A Wilk, Aaron J Rustagi, Arjun Zhao, Nancy Q Rogers, Angela J |
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| Cites_doi | 10.1101/2020.09.27.312538 10.1002/jha2.44 10.1038/s41587-020-0591-3 10.4269/ajtmh.20-0721 |
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| Copyright | 2020. Notwithstanding the ProQuest Terms and conditions, you may use this content in accordance with the associated terms available at https://www.biorxiv.org/content/10.1101/2020.10.15.339473v1 2020, Posted by Cold Spring Harbor Laboratory |
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| DOI | 10.1101/2020.10.15.339473 |
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| References | La Manno (2020.10.15.339473v1.3) 2018; 560 Francesconi (2020.10.15.339473v1.9) 2019; 8 Wilk (2020.10.15.339473v1.2) 2020; 26 Di Tullio (2020.10.15.339473v1.8) 2011; 108 DeKoter, Singh (2020.10.15.339473v1.5) 2000; 288 Lüke (2020.10.15.339473v1.12) 2020 Lee, Teo, Peripheral (2020.10.15.339473v1.11) 2020 Alquicira-Hernandez, Powell, Phan (2020.10.15.339473v1.1) 2020 Nazarullah, Liang, Villarreal, Higgins, Mais (2020.10.15.339473v1.10) 2020; 154 Rapino (2020.10.15.339473v1.7) 2013; 3 Bergen, Lange, Peidli, Wolf, Theis (2020.10.15.339473v1.4) 2020 Graf (2020.10.15.339473v1.6) 2011; 9 |
| References_xml | – volume: 560 start-page: 494 year: 2018 end-page: 498 ident: 2020.10.15.339473v1.3 article-title: RNA velocity of single cells publication-title: Nature – volume: 9 start-page: 504 year: 2011 end-page: 516 ident: 2020.10.15.339473v1.6 article-title: Historical origins of transdifferentiation and reprogramming publication-title: Cell Stem Cell – year: 2020 ident: 2020.10.15.339473v1.1 article-title: No evidence that plasmablasts transdifferentiate into developing neutrophils in severe COVID-19 disease doi: 10.1101/2020.09.27.312538 – volume: 8 year: 2019 ident: 2020.10.15.339473v1.9 article-title: Single cell RNA-seq identifies the origins of heterogeneity in efficient cell transdifferentiation and reprogramming publication-title: Elife – volume: 154 start-page: 319 year: 2020 end-page: 329 ident: 2020.10.15.339473v1.10 article-title: Peripheral Blood Examination Findings in SARS-CoV-2 Infection publication-title: Am. J. Clin. Pathol – year: 2020 ident: 2020.10.15.339473v1.12 article-title: Coronavirus disease 2019 induces multi-lineage, morphologic changes in peripheral blood cells publication-title: EJHaem doi: 10.1002/jha2.44 – year: 2020 ident: 2020.10.15.339473v1.4 article-title: Generalizing RNA velocity to transient cell states through dynamical modeling publication-title: Nat. Biotechnol doi: 10.1038/s41587-020-0591-3 – volume: 26 start-page: 1070 year: 2020 end-page: 1076 ident: 2020.10.15.339473v1.2 article-title: A single-cell atlas of the peripheral immune response in patients with severe COVID-19 publication-title: Nat. Med – volume: 288 start-page: 1439 year: 2000 end-page: 1441 ident: 2020.10.15.339473v1.5 article-title: Regulation of B lymphocyte and macrophage development by graded expression of PU. 1 publication-title: Science – year: 2020 ident: 2020.10.15.339473v1.11 article-title: Blood Smear Demonstration of Lymphocyte Changes in Severe COVID-19 publication-title: Am. J. Trop. Med. Hyg doi: 10.4269/ajtmh.20-0721 – volume: 3 start-page: 1153 year: 2013 end-page: 1163 ident: 2020.10.15.339473v1.7 article-title: C/EBPα induces highly efficient macrophage transdifferentiation of B lymphoma and leukemia cell lines and impairs their tumorigenicity publication-title: Cell Rep – volume: 108 start-page: 17016 year: 2011 end-page: 17021 ident: 2020.10.15.339473v1.8 article-title: CCAAT/enhancer binding protein α (C/EBPα)-induced transdifferentiation of pre-B cells into macrophages involves no overt retrodifferentiation publication-title: Proceedings of the National Academy of Sciences |
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| Snippet | Abstract We thank Alquicira-Hernandez et al. for their reanalysis of our single-cell transcriptomic dataset profiling peripheral immune responses to severe... We thank Alquicira-Hernandez et al. for their reanalysis of our single-cell transcriptomic dataset profiling peripheral immune responses to severe COVID-19. We... |
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| SubjectTerms | Coronaviruses COVID-19 Immune response Immunology Leukocytes (neutrophilic) |
| Title | Additional analyses exploring the hypothesized transdifferentiation of plasmablasts to developing neutrophils in severe COVID-19 |
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