Combined in situ hybridization chain reaction and immunostaining to visualize gene expression in whole-mount Drosophila central nervous systems

Methods to visualize gene expression in the Drosophila central nervous system are important in fly neurogenetic studies. In this chapter, we describe a detailed protocol that sequentially combines in situ hybridization chain reaction (HCR) and immunostaining to detect mRNA and protein expression in...

Full description

Bibliographic Details

Published in:	bioRxiv
Main Authors:	Duckhorn, Julia C, Junker, Ian P, Ding, Yun, Shirangi, Troy R
Format:	Paper
Language:	English
Published:	Cold Spring Harbor Cold Spring Harbor Laboratory Press 10.08.2021 Cold Spring Harbor Laboratory
Edition:	1.1
Subjects:	Central nervous system Drosophila Gene expression Hybridization Insects Kinases Nervous system Neuroscience
ISSN:	2692-8205, 2692-8205
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!